Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IEE84_RS01590 Genome accession   NZ_CP061739
Coordinates   367942..369063 (-) Length   373 a.a.
NCBI ID   WP_191114665.1    Uniprot ID   -
Organism   Psychrobacter sp. 28M-43     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 362942..374063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEE84_RS01560 (IEE84_01560) - 363441..363776 (+) 336 WP_057758239.1 c-type cytochrome -
  IEE84_RS01565 (IEE84_01565) - 364095..364763 (+) 669 WP_101205531.1 c-type cytochrome -
  IEE84_RS01570 (IEE84_01570) - 365117..366250 (+) 1134 WP_191114663.1 hypothetical protein -
  IEE84_RS01575 (IEE84_01575) - 366238..366609 (+) 372 WP_224737840.1 RnfH family protein -
  IEE84_RS01580 (IEE84_01580) - 366644..367063 (-) 420 WP_191114664.1 outer membrane protein assembly factor BamE -
  IEE84_RS01585 (IEE84_01585) fur 367381..367815 (+) 435 WP_057758249.1 ferric iron uptake transcriptional regulator -
  IEE84_RS01590 (IEE84_01590) pilU 367942..369063 (-) 1122 WP_191114665.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IEE84_RS01595 (IEE84_01595) pilT 369196..370251 (-) 1056 WP_179798169.1 type IV pilus twitching motility protein PilT Machinery gene
  IEE84_RS01600 (IEE84_01600) - 370594..371310 (+) 717 WP_224737843.1 YggS family pyridoxal phosphate-dependent enzyme -
  IEE84_RS01605 (IEE84_01605) - 371357..372631 (-) 1275 WP_057758257.1 hemolysin family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41674.86 Da        Isoelectric Point: 7.1151

>NTDB_id=484424 IEE84_RS01590 WP_191114665.1 367942..369063(-) (pilU) [Psychrobacter sp. 28M-43]
MDIDKLLQLMISKNGSDLFITADVAPSMKVNGKILPVGRTPLSAEQTMRLVKGVMTPSQRKEFEETNECQFAISDEAQQA
RFRVSAFVQRDMAGMILRKIENNIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLRAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRDSGMQTFDQ
ALFDLYEQGEITYKEAILHADSPNDLRLKIKLSSKNGKANLDEGADSLSLDVS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=484424 IEE84_RS01590 WP_191114665.1 367942..369063(-) (pilU) [Psychrobacter sp. 28M-43]
ATGGACATTGATAAACTATTACAGTTAATGATCAGCAAAAATGGCTCTGACCTTTTTATTACTGCTGACGTAGCACCTTC
CATGAAAGTGAATGGAAAAATTTTACCTGTCGGTAGAACACCTTTAAGTGCTGAGCAGACCATGAGATTGGTCAAAGGTG
TGATGACGCCAAGCCAACGAAAAGAGTTTGAAGAAACCAATGAATGTCAATTTGCCATCTCTGATGAAGCACAGCAAGCT
CGCTTTCGAGTCAGTGCATTTGTACAACGCGACATGGCAGGAATGATTTTACGGAAAATTGAAAATAATATTCCAACGGT
TGAAGAGTTACGTTTACCGCCAGCGCTAAAAGAATTGGCCATGAAAAAACGCGGTATTATTCTATTAGTTGGCGCAACGG
GTACAGGTAAATCCACTACCCTTGCAGCAATGATAGGACATCGCAATCAAAACTCTCATGGTCATATTATTACGATAGAA
GACCCTATTGAGTTTGTTCATAAACATCGCGGCTGTATTATTACCCAGCGTGAAGTTGGTATCGATACCCATTCATTTGA
AGCAGGCCTAAAAAATACGCTACGCCAAGCACCCGATGTCATCTTAATTGGCGAGATTCGTAACCGTGAAGTCATGAGCT
ACGCTATCCAGTACGCAGAAACAGGTCATTTAGTATTTGCAACGTTGCACGCAAACAATGCGAACCAAGCAATTGACCGT
ATTATTCACTTCTTTGAAACTAGTCGCCATAACCAGTTGTTTATGGATTTGTCGTTGAACTTGCGAGCTATTATCGCACA
GCAACTTATCCCCACACCTGATGGTAAAGGAAGACGTGCTGCTATCGAAATTTTGCTAAATTCGCAGCTGATCAGTGACT
ATATCCGTAAAGGGGAGGTCCATGAAATCAAAGATGTGATGACACGTTCACGAGACAGTGGCATGCAAACCTTTGATCAG
GCGCTTTTTGATTTGTATGAACAAGGAGAGATAACGTATAAAGAAGCGATCTTACATGCTGACTCTCCTAACGATCTACG
CCTCAAAATTAAGCTCAGCAGCAAAAATGGCAAGGCTAATTTAGATGAAGGTGCCGATAGTTTATCGTTAGATGTGAGCT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.56

100

0.676
  pilU Acinetobacter baylyi ADP1

66.201

95.979

0.635
  pilU Vibrio cholerae strain A1552

52.174

98.66

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.077

98.123

0.413
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.493

89.812

0.373
  pilT Vibrio cholerae strain A1552

41.493

89.812

0.373
  pilT Legionella pneumophila strain Lp02

41.369

90.08

0.373
  pilT Legionella pneumophila strain ERS1305867

41.369

90.08

0.373
  pilT Acinetobacter nosocomialis M2

40.233

91.957

0.37
  pilT Acinetobacter baumannii D1279779

40.233

91.957

0.37
  pilT Acinetobacter baumannii strain A118

40.233

91.957

0.37
  pilT Pseudomonas aeruginosa PAK

39.306

92.761

0.365