Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IDM46_RS02965 Genome accession   NZ_CP061714
Coordinates   640909..641946 (+) Length   345 a.a.
NCBI ID   WP_182822670.1    Uniprot ID   A0A7X0QLW0
Organism   Luteimonas sp. MC1825     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 635909..646946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IDM46_RS02930 (IDM46_02930) - 635912..636718 (-) 807 WP_185115239.1 kinase -
  IDM46_RS02935 (IDM46_02935) pdxH 636826..637422 (+) 597 WP_185114766.1 pyridoxamine 5'-phosphate oxidase -
  IDM46_RS02940 (IDM46_02940) - 637419..638249 (+) 831 WP_223878011.1 cobalamin-binding protein -
  IDM46_RS02945 (IDM46_02945) - 638299..638694 (+) 396 WP_221441768.1 DoxX family protein -
  IDM46_RS02950 (IDM46_02950) - 638704..639189 (-) 486 WP_185114768.1 DUF4426 domain-containing protein -
  IDM46_RS02955 (IDM46_02955) proC 639182..640027 (-) 846 WP_223878012.1 pyrroline-5-carboxylate reductase -
  IDM46_RS02960 (IDM46_02960) - 640078..640770 (-) 693 WP_185114770.1 YggS family pyridoxal phosphate-dependent enzyme -
  IDM46_RS02965 (IDM46_02965) pilT 640909..641946 (+) 1038 WP_182822670.1 type IV pilus twitching motility protein PilT Machinery gene
  IDM46_RS02970 (IDM46_02970) pilU 641959..643089 (+) 1131 WP_182822668.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IDM46_RS02975 (IDM46_02975) - 643204..643830 (+) 627 WP_182822666.1 superoxide dismutase -
  IDM46_RS02980 (IDM46_02980) - 644260..644721 (+) 462 WP_185114771.1 peptidylprolyl isomerase -
  IDM46_RS02985 (IDM46_02985) - 644761..646608 (-) 1848 WP_221441769.1 DUF4153 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38570.31 Da        Isoelectric Point: 6.7836

>NTDB_id=484336 IDM46_RS02965 WP_182822670.1 640909..641946(+) (pilT) [Luteimonas sp. MC1825]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHSLVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTVPSEVLTLEDLGTPRIFKELIDQPQGLILVTGPTGSGKSTTLAAMVDHINKTEYGHLLTIED
PIEFVHTAQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQQHGMMTLDQHL
QDLVKRGMVTRPQARDYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=484336 IDM46_RS02965 WP_182822670.1 640909..641946(+) (pilT) [Luteimonas sp. MC1825]
ATGGATATCGCTGAACTGCTGGCATTCTCGGTCAAGAACAAGGCGTCCGACCTGCACCTGTCCGCGGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTCCGCCGGATCAACATCCCGGCGCTGGACCACAAGCAGGTCCATTCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCCGGCCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCGGGCGCGGTGTTCCGCACGGTGCCGTCCGAGGTGCTGACGCTCGA
GGACCTCGGCACGCCGCGGATCTTCAAGGAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCCACGGGCT
CCGGCAAGTCGACCACGCTCGCCGCGATGGTCGACCACATCAACAAGACCGAGTACGGGCACCTCCTCACCATCGAGGAC
CCGATCGAGTTCGTGCACACCGCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACCCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACTACATCCTGGTCGGCGAGCTGCGTGACCTCGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGGCATCTGGTGTTCGGTACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCTGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCGGAGTCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTGGGCGGCGGGCGCACCGCGGCCTGGGAAATCATGGTCGGCATTCCCGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCAGCCATCCAGACCGGGCAGCAGCACGGCATGATGACCCTCGACCAGCATCTC
CAGGACCTGGTGAAGCGCGGCATGGTCACGCGGCCGCAGGCCCGTGACTACGCCAAGGACAAGCGGCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X0QLW0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.035

99.71

0.768
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

95.362

0.681
  pilT Vibrio cholerae strain A1552

71.429

95.362

0.681
  pilT Neisseria meningitidis 8013

64.62

99.13

0.641
  pilT Neisseria gonorrhoeae MS11

64.327

99.13

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Pseudomonas stutzeri DSM 10701

39.655

100

0.4
  pilU Vibrio cholerae strain A1552

38.793

100

0.391
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388