Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IDM46_RS02970 Genome accession   NZ_CP061714
Coordinates   641959..643089 (+) Length   376 a.a.
NCBI ID   WP_182822668.1    Uniprot ID   A0A7X0QNJ0
Organism   Luteimonas sp. MC1825     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 636959..648089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IDM46_RS02940 (IDM46_02940) - 637419..638249 (+) 831 WP_223878011.1 cobalamin-binding protein -
  IDM46_RS02945 (IDM46_02945) - 638299..638694 (+) 396 WP_221441768.1 DoxX family protein -
  IDM46_RS02950 (IDM46_02950) - 638704..639189 (-) 486 WP_185114768.1 DUF4426 domain-containing protein -
  IDM46_RS02955 (IDM46_02955) proC 639182..640027 (-) 846 WP_223878012.1 pyrroline-5-carboxylate reductase -
  IDM46_RS02960 (IDM46_02960) - 640078..640770 (-) 693 WP_185114770.1 YggS family pyridoxal phosphate-dependent enzyme -
  IDM46_RS02965 (IDM46_02965) pilT 640909..641946 (+) 1038 WP_182822670.1 type IV pilus twitching motility protein PilT Machinery gene
  IDM46_RS02970 (IDM46_02970) pilU 641959..643089 (+) 1131 WP_182822668.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IDM46_RS02975 (IDM46_02975) - 643204..643830 (+) 627 WP_182822666.1 superoxide dismutase -
  IDM46_RS02980 (IDM46_02980) - 644260..644721 (+) 462 WP_185114771.1 peptidylprolyl isomerase -
  IDM46_RS02985 (IDM46_02985) - 644761..646608 (-) 1848 WP_221441769.1 DUF4153 domain-containing protein -
  IDM46_RS02990 (IDM46_02990) - 646694..647254 (+) 561 WP_182822662.1 YqgE/AlgH family protein -
  IDM46_RS02995 (IDM46_02995) ruvX 647254..647715 (+) 462 WP_182822660.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41886.21 Da        Isoelectric Point: 6.8287

>NTDB_id=484337 IDM46_RS02970 WP_182822668.1 641959..643089(+) (pilU) [Luteimonas sp. MC1825]
MSAIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQNPLTPQQARDLVLNVMSPPQREEFERTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELNLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRGQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESTNLGMKTF
DQALFELYQAGEISYEDALRYADSANEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=484337 IDM46_RS02970 WP_182822668.1 641959..643089(+) (pilU) [Luteimonas sp. MC1825]
ATGAGCGCCATCGATTTCACCTCGTTCCTCAAGCTGATGGCCCACCAGAAGGCCTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCGATGAAGGTCCACGGCAAGATCTCGCCCATCACCCAGAACCCGCTCACGCCGCAGCAGGCGCGCGACCTGG
TGCTCAACGTCATGTCGCCGCCGCAGCGCGAGGAGTTCGAGCGCACGCACGAATGCAACTTCGCCATCGGTGTCTCGGGC
GTGGGCCGTTTCCGCGTGTCGTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGCATCGAGACCAGGATCCC
GACCGTCGAGGAGCTCAACCTCCCGCCGATCATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTGGGCG
CCACCGGCACCGGCAAGTCGACGTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTCAAGAACACCCTGCGCCAGGCGCCCGATGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCCATTTCGTTCGCTGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAGGACCGTCGCGGCCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGT
GGCGCAGCAGCTGATCCCCACGCCGGACGGCAAGGGGCGGCGCGTGGCGATGGAAATCATGCTCGGCACGCCGCTCGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAGTCGACCAACCTCGGCATGAAGACCTTC
GACCAGGCGCTGTTCGAGCTTTACCAGGCCGGCGAGATCTCCTACGAGGATGCGCTGCGCTACGCCGACTCCGCCAACGA
GGTGCGCCTGCGCATCAAGCTGGCGCAGGGCGGTGATGCGCGCACGTTGTCGCAGGGGCTGGACGGCGTCGAGGTGGCCG
AGGTGCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X0QNJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.392

97.606

0.668
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

54.494

94.681

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.109

90.691

0.391
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362
  pilT Acinetobacter nosocomialis M2

40.597

89.096

0.362
  pilT Acinetobacter baumannii D1279779

40.597

89.096

0.362
  pilT Acinetobacter baumannii strain A118

40.597

89.096

0.362