Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IC780_RS04345 Genome accession   NZ_CP061629
Coordinates   904148..905185 (-) Length   345 a.a.
NCBI ID   WP_019456823.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 884867..937892 904148..905185 within 0


Gene organization within MGE regions


Location: 884867..937892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC780_RS04245 (IC780_04240) rhtC 886646..887266 (+) 621 WP_004703321.1 threonine export protein RhtC -
  IC780_RS04250 (IC780_04245) - 887315..887602 (-) 288 WP_004703320.1 PA4642 family protein -
  IC780_RS04255 (IC780_04250) minE 887686..887958 (-) 273 WP_000896934.1 cell division topological specificity factor MinE -
  IC780_RS04260 (IC780_04255) minD 887961..888773 (-) 813 WP_001074362.1 septum site-determining protein MinD -
  IC780_RS04265 (IC780_04260) minC 888844..889566 (-) 723 WP_190977886.1 septum site-determining protein MinC -
  IC780_RS04270 (IC780_04265) - 889688..890734 (-) 1047 WP_151792555.1 preprotein translocase subunit SecA -
  IC780_RS04275 (IC780_04270) - 890749..891675 (-) 927 WP_151792554.1 acyltransferase -
  IC780_RS04280 (IC780_04275) - 891921..892574 (+) 654 WP_077170045.1 OmpA family protein -
  IC780_RS04285 (IC780_04280) - 892768..894807 (+) 2040 WP_039254369.1 UvrD-helicase domain-containing protein -
  IC780_RS04290 (IC780_04285) dut 894832..895284 (+) 453 WP_000868152.1 dUTP diphosphatase -
  IC780_RS04295 (IC780_04290) - 895480..896898 (+) 1419 WP_077170046.1 phosphomannomutase/phosphoglucomutase -
  IC780_RS04300 (IC780_04295) argB 896914..897822 (+) 909 WP_004703301.1 acetylglutamate kinase -
  IC780_RS04305 (IC780_04300) - 897941..898723 (+) 783 WP_077170047.1 GNAT family N-acetyltransferase -
  IC780_RS04310 (IC780_04305) - 898832..899680 (+) 849 WP_077170048.1 class II glutamine amidotransferase -
  IC780_RS04315 (IC780_04310) - 899931..900386 (+) 456 WP_004703295.1 bacteriohemerythrin -
  IC780_RS04320 (IC780_04315) - 900539..901606 (+) 1068 WP_143221137.1 hypothetical protein -
  IC780_RS04325 (IC780_04320) - 901606..901929 (+) 324 WP_019456825.1 RnfH family protein -
  IC780_RS04330 (IC780_04325) - 901985..902383 (-) 399 WP_004703289.1 outer membrane protein assembly factor BamE -
  IC780_RS04335 (IC780_04330) fur 902495..902932 (+) 438 WP_004703287.1 ferric iron uptake transcriptional regulator -
  IC780_RS04340 (IC780_04335) pilU 903002..904120 (-) 1119 WP_004703285.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IC780_RS04345 (IC780_04340) pilT 904148..905185 (-) 1038 WP_019456823.1 type IV pilus twitching motility protein PilT Machinery gene
  IC780_RS04350 (IC780_04345) - 905312..906004 (+) 693 WP_077170049.1 YggS family pyridoxal phosphate-dependent enzyme -
  IC780_RS04355 (IC780_04350) - 906111..906953 (+) 843 WP_019456821.1 undecaprenyl-diphosphate phosphatase -
  IC780_RS04360 (IC780_04355) - 907069..910665 (-) 3597 WP_151792553.1 SbcC/MukB-like Walker B domain-containing protein -
  IC780_RS04365 (IC780_04360) - 910675..911943 (-) 1269 WP_151792552.1 exonuclease SbcCD subunit D -
  IC780_RS04370 (IC780_04365) - 912108..912580 (+) 473 Protein_840 OsmC family protein -
  IC780_RS04375 (IC780_04370) - 912655..913032 (-) 378 WP_151792551.1 VOC family protein -
  IC780_RS04380 (IC780_04375) - 913407..913859 (+) 453 WP_049066316.1 ABZJ_00895 family protein -
  IC780_RS04385 (IC780_04380) - 913943..914848 (+) 906 WP_151792550.1 TIGR01777 family oxidoreductase -
  IC780_RS04390 (IC780_04385) - 914840..915955 (-) 1116 WP_151792549.1 NAD(P)/FAD-dependent oxidoreductase -
  IC780_RS04395 (IC780_04390) hemB 915981..916994 (-) 1014 WP_151792548.1 porphobilinogen synthase -
  IC780_RS04400 (IC780_04395) - 917137..917634 (+) 498 WP_004703260.1 thioesterase family protein -
  IC780_RS04405 (IC780_04400) - 917905..919056 (+) 1152 WP_019456813.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  IC780_RS04410 (IC780_04405) - 919063..920586 (+) 1524 WP_151792547.1 DHA2 family efflux MFS transporter permease subunit -
  IC780_RS04415 (IC780_04410) ggt 920892..922874 (+) 1983 WP_151792546.1 gamma-glutamyltransferase -
  IC780_RS04425 (IC780_04420) - 923450..923725 (-) 276 WP_151792545.1 hypothetical protein -
  IC780_RS21695 - 924505..924744 (+) 240 WP_223855044.1 hypothetical protein -
  IC780_RS04430 (IC780_04425) - 924713..927874 (+) 3162 WP_267459673.1 STY4851/ECs_5259 family protein -
  IC780_RS04435 (IC780_04430) - 927876..934172 (+) 6297 WP_151792543.1 DEAD/DEAH box helicase -
  IC780_RS04440 (IC780_04435) - 934499..934930 (+) 432 WP_223849254.1 hypothetical protein -
  IC780_RS04445 (IC780_04440) - 935183..935773 (+) 591 WP_151792541.1 hypothetical protein -
  IC780_RS04450 (IC780_04445) - 936076..937044 (+) 969 WP_012308653.1 IS30 family transposase -
  IC780_RS04455 (IC780_04450) - 937035..937358 (-) 324 WP_191029329.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38372.98 Da        Isoelectric Point: 6.6653

>NTDB_id=483689 IC780_RS04345 WP_019456823.1 904148..905185(-) (pilT) [Acinetobacter seifertii strain AS47]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KGLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=483689 IC780_RS04345 WP_019456823.1 904148..905185(-) (pilT) [Acinetobacter seifertii strain AS47]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCTTCCGATTTGCATTTATCTGCTGGCATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGCCGTATTAACTTACCAGCCTTAGAACATAAAGATGTCCACCGCCTTGTGTACGACA
TCATGAACGATAAACAGCGCCGTGATTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCACGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGTGCTGGCGCAGTATTTCGTACCATTCCATCTAAAGTACTCACCATGGA
AGATTTAGGCTTAGGTCAAATCTTTAAAGACATTTGTGACTATCCTCGCGGGATTGTCCTAGTTACAGGTCCAACAGGTT
CGGGTAAATCAACCACGCTTGCAGCGATGATGGACTACATTAATGAAAATCGTTATGACCATATTTTAACGGTTGAAGAC
CCAATCGAATTTGTACACCAATCAAAAAAATGCCTCATTAACCAGCGTGAAGTCCATCGTGATACACATGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGATCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACGATTCGTTTGG
CCCTTACAGCTGCCGAAACAGGTCACTTAGTATTTGGTACGCTTCATACCACATCTGCCGCTAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTACGTGCCATGCTGTCTGAATCTTTGCAAGCCGTTATTTCGCAAAC
TCTGCTTAAAAAGAACGGCGGCGGTCGTGTGGCAGCACATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATCC
GTGAAAACAAAGTCGCACAAATGTATTCAGCCATTCAAACTGGTGCTAACTATGGCATGACAACGCTTGACCAAAGCTTA
AAAGGTTTAGTGTCACGTGGTGTAATTAGCCCACAAACTGCACGTACTGCGGCAAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.42

100

0.994
  pilT Acinetobacter baumannii D1279779

99.13

100

0.991
  pilT Acinetobacter baumannii strain A118

99.13

100

0.991
  pilT Acinetobacter baylyi ADP1

88.081

99.71

0.878
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.131

97.391

0.693
  pilT Vibrio cholerae strain A1552

71.131

97.391

0.693
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388