Detailed information
Bioinformatically predicted
Overview
| Name | pilU | Type | Machinery gene |
| Locus tag | IC780_RS04340 | Genome accession | NZ_CP061629 |
| Coordinates | 903002..904120 (-) | Length | 372 a.a. |
| NCBI ID | WP_004703285.1 | Uniprot ID | N8S8R6 |
| Organism | Acinetobacter seifertii strain AS47 | ||
| Function | mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| ICE | 884867..937892 | 903002..904120 | within | 0 |
Gene organization within MGE regions
Location: 884867..937892
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IC780_RS04245 (IC780_04240) | rhtC | 886646..887266 (+) | 621 | WP_004703321.1 | threonine export protein RhtC | - |
| IC780_RS04250 (IC780_04245) | - | 887315..887602 (-) | 288 | WP_004703320.1 | PA4642 family protein | - |
| IC780_RS04255 (IC780_04250) | minE | 887686..887958 (-) | 273 | WP_000896934.1 | cell division topological specificity factor MinE | - |
| IC780_RS04260 (IC780_04255) | minD | 887961..888773 (-) | 813 | WP_001074362.1 | septum site-determining protein MinD | - |
| IC780_RS04265 (IC780_04260) | minC | 888844..889566 (-) | 723 | WP_190977886.1 | septum site-determining protein MinC | - |
| IC780_RS04270 (IC780_04265) | - | 889688..890734 (-) | 1047 | WP_151792555.1 | preprotein translocase subunit SecA | - |
| IC780_RS04275 (IC780_04270) | - | 890749..891675 (-) | 927 | WP_151792554.1 | acyltransferase | - |
| IC780_RS04280 (IC780_04275) | - | 891921..892574 (+) | 654 | WP_077170045.1 | OmpA family protein | - |
| IC780_RS04285 (IC780_04280) | - | 892768..894807 (+) | 2040 | WP_039254369.1 | UvrD-helicase domain-containing protein | - |
| IC780_RS04290 (IC780_04285) | dut | 894832..895284 (+) | 453 | WP_000868152.1 | dUTP diphosphatase | - |
| IC780_RS04295 (IC780_04290) | - | 895480..896898 (+) | 1419 | WP_077170046.1 | phosphomannomutase/phosphoglucomutase | - |
| IC780_RS04300 (IC780_04295) | argB | 896914..897822 (+) | 909 | WP_004703301.1 | acetylglutamate kinase | - |
| IC780_RS04305 (IC780_04300) | - | 897941..898723 (+) | 783 | WP_077170047.1 | GNAT family N-acetyltransferase | - |
| IC780_RS04310 (IC780_04305) | - | 898832..899680 (+) | 849 | WP_077170048.1 | class II glutamine amidotransferase | - |
| IC780_RS04315 (IC780_04310) | - | 899931..900386 (+) | 456 | WP_004703295.1 | bacteriohemerythrin | - |
| IC780_RS04320 (IC780_04315) | - | 900539..901606 (+) | 1068 | WP_143221137.1 | hypothetical protein | - |
| IC780_RS04325 (IC780_04320) | - | 901606..901929 (+) | 324 | WP_019456825.1 | RnfH family protein | - |
| IC780_RS04330 (IC780_04325) | - | 901985..902383 (-) | 399 | WP_004703289.1 | outer membrane protein assembly factor BamE | - |
| IC780_RS04335 (IC780_04330) | fur | 902495..902932 (+) | 438 | WP_004703287.1 | ferric iron uptake transcriptional regulator | - |
| IC780_RS04340 (IC780_04335) | pilU | 903002..904120 (-) | 1119 | WP_004703285.1 | PilT/PilU family type 4a pilus ATPase | Machinery gene |
| IC780_RS04345 (IC780_04340) | pilT | 904148..905185 (-) | 1038 | WP_019456823.1 | type IV pilus twitching motility protein PilT | Machinery gene |
| IC780_RS04350 (IC780_04345) | - | 905312..906004 (+) | 693 | WP_077170049.1 | YggS family pyridoxal phosphate-dependent enzyme | - |
| IC780_RS04355 (IC780_04350) | - | 906111..906953 (+) | 843 | WP_019456821.1 | undecaprenyl-diphosphate phosphatase | - |
| IC780_RS04360 (IC780_04355) | - | 907069..910665 (-) | 3597 | WP_151792553.1 | SbcC/MukB-like Walker B domain-containing protein | - |
| IC780_RS04365 (IC780_04360) | - | 910675..911943 (-) | 1269 | WP_151792552.1 | exonuclease SbcCD subunit D | - |
| IC780_RS04370 (IC780_04365) | - | 912108..912580 (+) | 473 | Protein_840 | OsmC family protein | - |
| IC780_RS04375 (IC780_04370) | - | 912655..913032 (-) | 378 | WP_151792551.1 | VOC family protein | - |
| IC780_RS04380 (IC780_04375) | - | 913407..913859 (+) | 453 | WP_049066316.1 | ABZJ_00895 family protein | - |
| IC780_RS04385 (IC780_04380) | - | 913943..914848 (+) | 906 | WP_151792550.1 | TIGR01777 family oxidoreductase | - |
| IC780_RS04390 (IC780_04385) | - | 914840..915955 (-) | 1116 | WP_151792549.1 | NAD(P)/FAD-dependent oxidoreductase | - |
| IC780_RS04395 (IC780_04390) | hemB | 915981..916994 (-) | 1014 | WP_151792548.1 | porphobilinogen synthase | - |
| IC780_RS04400 (IC780_04395) | - | 917137..917634 (+) | 498 | WP_004703260.1 | thioesterase family protein | - |
| IC780_RS04405 (IC780_04400) | - | 917905..919056 (+) | 1152 | WP_019456813.1 | EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit | - |
| IC780_RS04410 (IC780_04405) | - | 919063..920586 (+) | 1524 | WP_151792547.1 | DHA2 family efflux MFS transporter permease subunit | - |
| IC780_RS04415 (IC780_04410) | ggt | 920892..922874 (+) | 1983 | WP_151792546.1 | gamma-glutamyltransferase | - |
| IC780_RS04425 (IC780_04420) | - | 923450..923725 (-) | 276 | WP_151792545.1 | hypothetical protein | - |
| IC780_RS21695 | - | 924505..924744 (+) | 240 | WP_223855044.1 | hypothetical protein | - |
| IC780_RS04430 (IC780_04425) | - | 924713..927874 (+) | 3162 | WP_267459673.1 | STY4851/ECs_5259 family protein | - |
| IC780_RS04435 (IC780_04430) | - | 927876..934172 (+) | 6297 | WP_151792543.1 | DEAD/DEAH box helicase | - |
| IC780_RS04440 (IC780_04435) | - | 934499..934930 (+) | 432 | WP_223849254.1 | hypothetical protein | - |
| IC780_RS04445 (IC780_04440) | - | 935183..935773 (+) | 591 | WP_151792541.1 | hypothetical protein | - |
| IC780_RS04450 (IC780_04445) | - | 936076..937044 (+) | 969 | WP_012308653.1 | IS30 family transposase | - |
| IC780_RS04455 (IC780_04450) | - | 937035..937358 (-) | 324 | WP_191029329.1 | hypothetical protein | - |
Sequence
Protein
Download Length: 372 a.a. Molecular weight: 41966.15 Da Isoelectric Point: 6.6944
>NTDB_id=483688 IC780_RS04340 WP_004703285.1 903002..904120(-) (pilU) [Acinetobacter seifertii strain AS47]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ
Nucleotide
Download Length: 1119 bp
>NTDB_id=483688 IC780_RS04340 WP_004703285.1 903002..904120(-) (pilU) [Acinetobacter seifertii strain AS47]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTAATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTCGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAGCGTGATATGCCGGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCTTCTGTGCTTAAAGATTTATCAATGACCAAACGTGGCATTATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATTATTACTATTGAA
GACCCTATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTAGGTATTGATACTGACTCGTTCGA
AATTGCTTTAAAAAATACGTTACGACAAGCGCCCGATGTCATCTTAATTGGTGAGATCCGCTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTTAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTAACTTTTGATCGACAGTAA
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTAATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTCGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAGCGTGATATGCCGGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCTTCTGTGCTTAAAGATTTATCAATGACCAAACGTGGCATTATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATTATTACTATTGAA
GACCCTATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTAGGTATTGATACTGACTCGTTCGA
AATTGCTTTAAAAAATACGTTACGACAAGCGCCCGATGTCATCTTAATTGGTGAGATCCGCTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTTAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTAACTTTTGATCGACAGTAA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilU | Acinetobacter baylyi ADP1 |
75.132 |
100 |
0.763 |
| pilU | Pseudomonas stutzeri DSM 10701 |
68.478 |
98.925 |
0.677 |
| pilU | Vibrio cholerae strain A1552 |
55.271 |
94.355 |
0.522 |
| pilT | Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 |
41.972 |
95.43 |
0.401 |