Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IC799_RS13870 Genome accession   NZ_CP061550
Coordinates   3017596..3018633 (+) Length   345 a.a.
NCBI ID   WP_025469189.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS79     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3012596..3023633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC799_RS13860 (IC799_13855) - 3015828..3016670 (-) 843 WP_191028608.1 undecaprenyl-diphosphate phosphatase -
  IC799_RS13865 (IC799_13860) - 3016777..3017469 (-) 693 WP_151761021.1 YggS family pyridoxal phosphate-dependent enzyme -
  IC799_RS13870 (IC799_13865) pilT 3017596..3018633 (+) 1038 WP_025469189.1 type IV pilus twitching motility protein PilT Machinery gene
  IC799_RS13875 (IC799_13870) pilU 3018661..3019779 (+) 1119 WP_004703285.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IC799_RS13880 (IC799_13875) fur 3019850..3020287 (-) 438 WP_004703287.1 ferric iron uptake transcriptional regulator -
  IC799_RS13885 (IC799_13880) - 3020399..3020797 (+) 399 WP_004703289.1 outer membrane protein assembly factor BamE -
  IC799_RS13890 (IC799_13885) - 3020853..3021176 (-) 324 WP_019456825.1 RnfH family protein -
  IC799_RS13895 (IC799_13890) - 3021176..3022243 (-) 1068 WP_191012727.1 hypothetical protein -
  IC799_RS13900 (IC799_13895) - 3022396..3022851 (-) 456 WP_004703295.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38387.01 Da        Isoelectric Point: 6.6653

>NTDB_id=483090 IC799_RS13870 WP_025469189.1 3017596..3018633(+) (pilT) [Acinetobacter seifertii strain AS79]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KALVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=483090 IC799_RS13870 WP_025469189.1 3017596..3018633(+) (pilT) [Acinetobacter seifertii strain AS79]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACACTTATCTGCTGGCATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGCCGTATTAACTTACCAGCCTTAGAACATAAAGATGTCCACCGCCTTGTTTACGACA
TCATGAACGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACAGACTTTTCTTTTGAAGTACCAAATGTTGCACGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGTGCTGGCGCAGTATTTCGTACCATTCCATCTAAAGTACTGACCATGGA
AGATTTAGGCTTAGGCCAAATCTTTAAAGATATTTGCGACTATCCTCGCGGGATTGTACTGGTGACAGGGCCAACAGGTT
CGGGTAAATCAACCACGCTTGCGGCAATGATGGACTATATTAATGAAAATCGTTATGACCATATTTTAACGGTTGAAGAC
CCCATCGAATTTGTACACCAGTCAAAAAAATGCCTCATTAACCAGCGTGAAGTCCATCGTGATACACATGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACGATTCGTTTAG
CTCTTACAGCTGCCGAAACAGGTCACTTAGTATTTGGTACGCTTCATACCACATCTGCCGCTAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTACGTGCCATGCTGTCTGAATCTTTGCAAGCCGTTATTTCGCAAAC
TTTGCTTAAAAAGAACGGCGGCGGTCGTGTGGCAGCACATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATCC
GTGAAAACAAAGTCGCACAAATGTATTCAGCTATTCAAACTGGTGCTAACTATGGCATGACAACGCTTGACCAAAGCTTA
AAAGCTTTAGTGTCACGTGGTGTAATTAGCCCACAAACTGCACGTACTGCGGCAAAACAACCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.13

100

0.991
  pilT Acinetobacter baumannii D1279779

98.841

100

0.988
  pilT Acinetobacter baumannii strain A118

98.841

100

0.988
  pilT Acinetobacter baylyi ADP1

88.081

99.71

0.878
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.131

97.391

0.693
  pilT Vibrio cholerae strain A1552

71.131

97.391

0.693
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388