Detailed information NTG0483091

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IC799_RS13875
• Genome accession: NZ_CP061550
• Coordinates: 3018661..3019779 (+)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain AS79
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3013661..3024779

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC799_RS13860 (IC799_13855)	-	3015828..3016670 (-)	843	WP_191028608.1	undecaprenyl-diphosphate phosphatase	-
IC799_RS13865 (IC799_13860)	-	3016777..3017469 (-)	693	WP_151761021.1	YggS family pyridoxal phosphate-dependent enzyme	-
IC799_RS13870 (IC799_13865)	pilT	3017596..3018633 (+)	1038	WP_025469189.1	type IV pilus twitching motility protein PilT	Machinery gene
IC799_RS13875 (IC799_13870)	pilU	3018661..3019779 (+)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IC799_RS13880 (IC799_13875)	fur	3019850..3020287 (-)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
IC799_RS13885 (IC799_13880)	-	3020399..3020797 (+)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
IC799_RS13890 (IC799_13885)	-	3020853..3021176 (-)	324	WP_019456825.1	RnfH family protein	-
IC799_RS13895 (IC799_13890)	-	3021176..3022243 (-)	1068	WP_191012727.1	hypothetical protein	-
IC799_RS13900 (IC799_13895)	-	3022396..3022851 (-)	456	WP_004703295.1	bacteriohemerythrin	-
IC799_RS13905 (IC799_13900)	-	3023102..3023950 (-)	849	WP_019456827.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=483091 IC799_RS13875 WP_004703285.1 3018661..3019779(+) (pilU) [Acinetobacter seifertii strain AS79]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=483091 IC799_RS13875 WP_004703285.1 3018661..3019779(+) (pilU) [Acinetobacter seifertii strain AS79]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
TATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGTGAAGTAATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTTGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAACGCGATATGCCAGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCCTCTGTGCTTAAAGATTTATCAATGACCAAACGCGGCATTATTATTTTTGTAGGCGCAACAG
GTACTGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATCATTACTATTGAA
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTCGGTATTGATACTGACTCGTTTGA
AATTGCTTTGAAAAATACTTTACGACAAGCACCTGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTCAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCGGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACCTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTGACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401