Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IAR63_RS11305 Genome accession   NZ_CP060822
Coordinates   2548381..2549457 (-) Length   358 a.a.
NCBI ID   WP_187705345.1    Uniprot ID   A0A7H0EXI4
Organism   Cylindrospermopsis curvispora GIHE-G1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2543381..2554457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAR63_RS11285 (IAR63_11285) - 2543753..2544925 (-) 1173 WP_187705343.1 helix-turn-helix domain-containing protein -
  IAR63_RS11290 (IAR63_11290) - 2545791..2546000 (+) 210 WP_235678250.1 hypothetical protein -
  IAR63_RS11295 (IAR63_11295) - 2546060..2547064 (+) 1005 WP_187707447.1 saccharopine dehydrogenase-like oxidoreductase -
  IAR63_RS11300 (IAR63_11300) - 2547093..2548352 (-) 1260 WP_187705344.1 type II secretion system F family protein -
  IAR63_RS11305 (IAR63_11305) pilT 2548381..2549457 (-) 1077 WP_187705345.1 type IV pilus twitching motility protein PilT Machinery gene
  IAR63_RS11310 (IAR63_11310) pilB/pilB1 2549475..2551466 (-) 1992 WP_187705346.1 GspE/PulE family protein Machinery gene
  IAR63_RS11315 (IAR63_11315) - 2551596..2551781 (+) 186 WP_187705347.1 hypothetical protein -
  IAR63_RS11320 (IAR63_11320) grpE 2552022..2552717 (+) 696 WP_407927138.1 nucleotide exchange factor GrpE -
  IAR63_RS11325 (IAR63_11325) dnaJ 2553014..2554144 (+) 1131 WP_187705349.1 molecular chaperone DnaJ -
  IAR63_RS11330 (IAR63_11330) - 2554155..2554406 (+) 252 WP_187705350.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39931.48 Da        Isoelectric Point: 7.4149

>NTDB_id=478627 IAR63_RS11305 WP_187705345.1 2548381..2549457(-) (pilT) [Cylindrospermopsis curvispora GIHE-G1]
MEYTIKELLQNLVDMGGSDLHLSAGLRPYFRIGGKLTPLGNEILTPDECEKLIFSMLNKDQRHRLEKTWELDCSYEIKGL
ARFRVNVYKERGNYAACLRVLSYKIPNFEELGLPKIIREIAEKPRGLILVTGPTGSGKTTTLAAMIDLINQNRAEHILTI
EDPIEFVHEPIKSLIHQRQLGEDTRSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVLATLHTNSAAQTID
RIIDVFPAERQMQIRVQLSNSLLAVFSQILVPRKNVKPGGSSRVMAQEIMIVTPAIANLIREGKIAQIYSFIQTGGKLGM
QTLEKALCELFKAGLISQELALSRTSRPDEVQRLIGIL

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=478627 IAR63_RS11305 WP_187705345.1 2548381..2549457(-) (pilT) [Cylindrospermopsis curvispora GIHE-G1]
ATGGAATACACAATTAAAGAACTGCTGCAAAATTTAGTAGATATGGGTGGTTCAGACCTGCATCTATCTGCAGGTTTAAG
ACCCTATTTTCGCATTGGTGGAAAACTCACTCCCTTAGGTAATGAGATATTAACACCAGATGAATGTGAAAAACTGATTT
TTAGTATGTTGAATAAGGACCAGCGTCACAGGTTAGAAAAAACCTGGGAGTTAGATTGTTCTTATGAAATCAAAGGTCTA
GCACGTTTTCGAGTTAACGTCTATAAAGAAAGGGGCAATTATGCAGCTTGTTTACGGGTTTTAAGTTATAAAATTCCCAA
TTTTGAAGAGTTAGGTTTACCCAAGATTATTCGAGAAATAGCAGAAAAACCCAGAGGACTAATTCTTGTTACAGGTCCAA
CCGGTTCTGGTAAAACCACTACCCTAGCTGCAATGATTGATTTAATCAACCAAAATCGAGCTGAACATATTCTAACCATA
GAAGACCCAATAGAATTTGTGCATGAGCCAATTAAAAGTTTAATCCACCAACGACAATTAGGTGAAGATACACGGAGTTT
TGCCAATGCCTTAAAAGCAGCTTTACGGGAAGATCCAGATATTATTCTCGTGGGGGAAATGCGAGACCTAGAAACGATTT
CTCTAGCTATTTCTGCTGCAGAAACAGGTCATTTGGTCTTAGCCACATTACATACTAATTCAGCAGCACAGACCATTGAT
AGAATCATTGATGTTTTTCCAGCGGAACGACAAATGCAAATTAGAGTACAATTATCTAACTCCCTGTTGGCAGTTTTCAG
TCAAATCCTAGTACCTAGAAAAAACGTTAAACCAGGTGGATCTAGTCGGGTGATGGCACAGGAAATTATGATAGTTACTC
CAGCAATTGCTAACTTAATAAGGGAGGGAAAAATAGCCCAGATATATTCATTTATTCAAACAGGAGGTAAATTGGGAATG
CAAACCTTAGAAAAAGCCCTATGTGAGCTATTTAAAGCTGGTCTTATTTCTCAAGAACTAGCACTTTCTAGGACTTCTAG
ACCGGATGAAGTGCAACGGTTAATTGGTATTTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H0EXI4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

54.783

96.369

0.528
  pilT Legionella pneumophila strain Lp02

55.786

94.134

0.525
  pilT Legionella pneumophila strain ERS1305867

55.786

94.134

0.525
  pilT Pseudomonas aeruginosa PAK

53.913

96.369

0.52
  pilT Pseudomonas stutzeri DSM 10701

53.623

96.369

0.517
  pilT Acinetobacter baumannii strain A118

53.043

96.369

0.511
  pilT Acinetobacter baumannii D1279779

53.043

96.369

0.511
  pilT Acinetobacter nosocomialis M2

53.043

96.369

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.429

97.765

0.503
  pilT Vibrio cholerae strain A1552

52.059

94.972

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.059

94.972

0.494
  pilT Neisseria meningitidis 8013

51.176

94.972

0.486
  pilT Neisseria gonorrhoeae MS11

51.176

94.972

0.486
  pilU Acinetobacter baylyi ADP1

41.595

98.045

0.408
  pilU Pseudomonas stutzeri DSM 10701

40.282

99.162

0.399
  pilU Vibrio cholerae strain A1552

37.322

98.045

0.366