Detailed information NTG0478628

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LYSZa5_RS14280
• Genome accession: NZ_CP071730
• Coordinates: 3052553..3053701 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain LYSZa5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3047553..3058701

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LYSZa5_RS14255 (LYSZa5_14255)	-	3048704..3049000 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
LYSZa5_RS14260 (LYSZa5_14260)	-	3048997..3049590 (-)	594	WP_003084544.1	YggT family protein	-
LYSZa5_RS14265 (LYSZa5_14265)	proC	3049601..3050422 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
LYSZa5_RS14270 (LYSZa5_14270)	-	3050434..3051126 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
LYSZa5_RS14275 (LYSZa5_14275)	pilT	3051341..3052375 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LYSZa5_RS14280 (LYSZa5_14280)	pilU	3052553..3053701 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
LYSZa5_RS14285 (LYSZa5_14285)	czcD	3053709..3054608 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LYSZa5_RS14290 (LYSZa5_14290)	-	3054762..3055166 (+)	405	WP_003084560.1	NINE protein	-
LYSZa5_RS14295 (LYSZa5_14295)	-	3055409..3056782 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
LYSZa5_RS14300 (LYSZa5_14300)	-	3056779..3057963 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=478628 LYSZa5_RS14280 WP_003100959.1 3052553..3053701(+) (pilU) [Pseudomonas aeruginosa strain LYSZa5]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=478628 LYSZa5_RS14280 WP_003100959.1 3052553..3053701(+) (pilU) [Pseudomonas aeruginosa strain LYSZa5]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382