Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   IAR63_RS11310 Genome accession   NZ_CP060822
Coordinates   2549475..2551466 (-) Length   663 a.a.
NCBI ID   WP_187705346.1    Uniprot ID   A0A7H0EXI5
Organism   Cylindrospermopsis curvispora GIHE-G1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2544475..2556466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAR63_RS11290 (IAR63_11290) - 2545791..2546000 (+) 210 WP_235678250.1 hypothetical protein -
  IAR63_RS11295 (IAR63_11295) - 2546060..2547064 (+) 1005 WP_187707447.1 saccharopine dehydrogenase-like oxidoreductase -
  IAR63_RS11300 (IAR63_11300) - 2547093..2548352 (-) 1260 WP_187705344.1 type II secretion system F family protein -
  IAR63_RS11305 (IAR63_11305) pilT 2548381..2549457 (-) 1077 WP_187705345.1 type IV pilus twitching motility protein PilT Machinery gene
  IAR63_RS11310 (IAR63_11310) pilB/pilB1 2549475..2551466 (-) 1992 WP_187705346.1 GspE/PulE family protein Machinery gene
  IAR63_RS11315 (IAR63_11315) - 2551596..2551781 (+) 186 WP_187705347.1 hypothetical protein -
  IAR63_RS11320 (IAR63_11320) grpE 2552022..2552717 (+) 696 WP_407927138.1 nucleotide exchange factor GrpE -
  IAR63_RS11325 (IAR63_11325) dnaJ 2553014..2554144 (+) 1131 WP_187705349.1 molecular chaperone DnaJ -
  IAR63_RS11330 (IAR63_11330) - 2554155..2554406 (+) 252 WP_187705350.1 sulfurtransferase TusA family protein -
  IAR63_RS11335 (IAR63_11335) rsgA 2554432..2555490 (+) 1059 WP_187705351.1 small ribosomal subunit biogenesis GTPase RsgA -
  IAR63_RS11345 (IAR63_11345) - 2555683..2556231 (-) 549 WP_061546595.1 NYN domain-containing protein -

Sequence

Protein


Download         Length: 663 a.a.        Molecular weight: 74623.55 Da        Isoelectric Point: 5.7873

>NTDB_id=478628 IAR63_RS11310 WP_187705346.1 2549475..2551466(-) (pilB/pilB1) [Cylindrospermopsis curvispora GIHE-G1]
MIYSVKKRLDKTSAIQVNTLPVLNKLVKSGHIHREQMKLAVMESLESGKVLTDVLQSIARGKLDPQLIRECKKQHLFELK
ILYGVEYIDPEVNQMNWNIIRELIESLIPIDICRSYGLLPLGKEENQNPPQVLVAMVEPDNLAACDYLNRILQPPNWQFK
RLVIAAEDYEILINQYTKEQTQFTDISQDLESLSLEVMEQRLEEGDVDLTVAMKEAQDEPIINLVNKILFLALRDGASDI
HVEPQEEYLRIRFRNDGVLRESESIPPIPKHIIPAVTARIKIISNLDIAEKRLPQDGRIRRVFDGKKVDIRVSTLPNRYG
EKICLRILDNSSTKLPLDQIITDPHTLQVFKNIIHKPLGLILVTGPTGSGKTTSLYSALAELNDPGINISTVEDPVEYSL
PGLTQVQVIREKGLDFATVLRAFLRQDPDVLLVGETRDGETAKTAIEAALTGHLVFTTLHTIDAAGAIARLLEMGIEPFM
ISSSLIGVLAQRLVRRLCPTCRIPYNPTVQELGRYGLSLVQEGNVIFYKANTISSELIGKSPNKIKVCPNCYGTGYKGRC
GVYEVMAVTQKLQNLINQEADTEKIKEAALEEGMITLLGYSLNLVRQGITTLEEVERVVYTDRELIRELKVSRQKSLTCQ
CCDATLKPEWLDCPYCLTPRLIN

Nucleotide


Download         Length: 1992 bp        

>NTDB_id=478628 IAR63_RS11310 WP_187705346.1 2549475..2551466(-) (pilB/pilB1) [Cylindrospermopsis curvispora GIHE-G1]
ATGATTTACTCAGTTAAAAAACGTTTAGATAAAACTAGTGCTATTCAAGTTAACACTCTTCCTGTACTAAACAAACTAGT
TAAATCTGGTCATATTCATAGGGAACAGATGAAGCTAGCCGTGATGGAAAGTCTTGAATCTGGGAAAGTATTAACAGATG
TTTTACAATCTATAGCCAGAGGGAAGTTAGATCCTCAGTTAATCAGAGAATGTAAAAAACAGCATTTATTTGAATTAAAA
ATACTATATGGTGTAGAATATATAGATCCAGAAGTCAATCAAATGAACTGGAATATTATCAGAGAATTGATCGAGAGTTT
AATACCAATAGATATTTGTCGTAGTTATGGATTATTACCATTAGGAAAGGAAGAAAATCAGAATCCACCCCAGGTGTTAG
TAGCAATGGTTGAGCCAGATAATTTAGCGGCATGTGATTACCTTAATAGGATATTACAACCGCCAAACTGGCAATTTAAA
CGTCTAGTAATTGCTGCGGAAGATTACGAAATATTAATTAATCAATACACAAAAGAGCAAACTCAATTCACAGATATTAG
CCAAGATCTAGAAAGTTTAAGTCTTGAGGTTATGGAGCAGAGACTAGAAGAAGGAGATGTAGATTTGACAGTAGCCATGA
AAGAAGCTCAGGATGAGCCGATTATTAATCTTGTGAATAAAATTCTCTTTTTAGCTCTGCGAGATGGTGCTTCTGATATT
CACGTTGAACCTCAAGAAGAATATTTACGAATTCGGTTTCGCAACGATGGAGTACTGCGGGAGTCGGAATCTATTCCCCC
CATTCCCAAACATATTATTCCTGCAGTTACTGCGCGAATTAAAATTATCTCTAATCTAGATATTGCCGAAAAGAGATTGC
CCCAAGATGGCAGAATTCGCAGAGTATTTGATGGTAAAAAGGTAGATATCCGAGTCAGCACCTTACCCAACCGCTACGGA
GAAAAAATCTGTCTGAGGATTTTAGACAATTCCTCCACTAAATTACCCCTAGATCAAATAATTACTGATCCCCATACTTT
GCAGGTATTTAAAAATATTATTCATAAACCCCTAGGTTTAATTTTAGTCACAGGTCCAACCGGTTCTGGAAAAACTACCT
CCTTATATTCTGCTCTAGCAGAATTAAATGATCCGGGAATTAATATTAGTACAGTTGAAGATCCCGTAGAATACAGTTTA
CCGGGACTGACTCAAGTGCAGGTAATTCGAGAAAAGGGATTAGACTTTGCCACAGTTCTGCGAGCTTTTCTGCGACAAGA
CCCAGACGTGTTATTGGTGGGAGAAACACGAGATGGGGAAACTGCGAAAACCGCAATTGAAGCAGCACTAACCGGACATC
TAGTATTCACTACCTTGCATACTATAGATGCTGCAGGAGCGATCGCCCGCTTACTGGAAATGGGTATTGAACCATTTATG
ATTTCTAGTTCTCTAATTGGTGTATTAGCCCAGAGGTTAGTGCGTAGACTGTGTCCCACATGTCGAATTCCCTATAATCC
TACAGTTCAAGAACTAGGGAGATATGGTTTATCCCTAGTGCAAGAAGGCAATGTCATTTTCTATAAAGCTAATACCATAT
CATCAGAACTTATAGGAAAAAGCCCGAATAAAATCAAAGTTTGTCCTAACTGTTATGGTACTGGCTACAAAGGGCGTTGT
GGAGTGTATGAAGTTATGGCAGTTACGCAAAAACTACAAAATTTAATTAATCAAGAAGCTGACACAGAAAAAATTAAAGA
AGCAGCTTTAGAAGAGGGAATGATAACCCTATTGGGTTATAGTCTCAATCTAGTTCGTCAAGGCATAACGACCTTAGAAG
AAGTGGAGCGGGTGGTATATACAGATAGGGAATTGATAAGGGAATTAAAAGTCTCACGACAGAAGAGTCTCACATGTCAA
TGTTGTGATGCTACCCTAAAACCAGAGTGGTTAGATTGTCCTTATTGTTTGACCCCTAGATTAATTAATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(227-496)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H0EXI5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

55.657

98.643

0.549