Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H8B22_RS02915 Genome accession   NZ_CP060820
Coordinates   610616..611653 (-) Length   345 a.a.
NCBI ID   WP_187712631.1    Uniprot ID   -
Organism   Agrilutibacter terrestris strain II4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 605616..616653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8B22_RS02890 (H8B22_02890) - 606087..606653 (-) 567 WP_187712626.1 YqgE/AlgH family protein -
  H8B22_RS02895 (H8B22_02895) - 606748..608475 (+) 1728 WP_187712627.1 dipeptidyl-peptidase 3 family protein -
  H8B22_RS02900 (H8B22_02900) - 608477..609046 (+) 570 WP_187712628.1 DNA-3-methyladenine glycosylase I -
  H8B22_RS02905 (H8B22_02905) - 609125..609274 (+) 150 WP_187712629.1 hypothetical protein -
  H8B22_RS02910 (H8B22_02910) pilU 609351..610481 (-) 1131 WP_187712630.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H8B22_RS02915 (H8B22_02915) pilT 610616..611653 (-) 1038 WP_187712631.1 type IV pilus twitching motility protein PilT Machinery gene
  H8B22_RS02920 (H8B22_02920) - 611800..612480 (+) 681 WP_407060823.1 YggS family pyridoxal phosphate-dependent enzyme -
  H8B22_RS02925 (H8B22_02925) proC 612558..613394 (+) 837 WP_187712632.1 pyrroline-5-carboxylate reductase -
  H8B22_RS02930 (H8B22_02930) - 613402..613893 (+) 492 WP_187712633.1 DUF4426 domain-containing protein -
  H8B22_RS02935 (H8B22_02935) - 613924..614721 (-) 798 WP_187712634.1 ABC transporter substrate-binding protein -
  H8B22_RS02940 (H8B22_02940) pdxH 614718..615320 (-) 603 WP_187712635.1 pyridoxamine 5'-phosphate oxidase -
  H8B22_RS02945 (H8B22_02945) - 615376..615579 (-) 204 WP_187712636.1 dodecin family protein -
  H8B22_RS02950 (H8B22_02950) - 615691..616233 (+) 543 WP_225876260.1 shikimate kinase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38540.43 Da        Isoelectric Point: 6.9354

>NTDB_id=478606 H8B22_RS02915 WP_187712631.1 610616..611653(-) (pilT) [Agrilutibacter terrestris strain II4]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLDDLGCPRIFKEIIDQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATVHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRTAAWEIMVGIPAIRNLIREDKIAQMYSAIQTGQAHGMMTLDQHL
QDLVKRGLILKPQARMYAKDKRLFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=478606 H8B22_RS02915 WP_187712631.1 610616..611653(-) (pilT) [Agrilutibacter terrestris strain II4]
ATGGATATCGCCGAACTTCTGGCGTTTTCGGTCAAGAACAAGGCCTCCGACTTGCATCTGTCGGCCGGTCTCCCGCCGAT
GATCCGAGTGGACGGCGACGTCCGCCGCATCAACATTCCGGCGCTCGATCACAAGCAGGTCCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAAGAGTTCCTCGAGGTCGACTTCTCCTTCGAGATCCCCGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCGGAAGTGTTGACCCTCGA
CGACCTGGGCTGCCCGCGCATCTTCAAGGAAATCATCGACCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAATACGCGCACATCCTCTCCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTACACCGCGACACCCACGGTTTCAACGA
GGCGTTGCGCTCGGCCCTGCGTGAGGACCCCGACTACATCCTCGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGCGACCGTGCACACCTCTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTCCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGTC
GCTGCTGAAGAAGGTCGGTGGCGGCCGGACCGCGGCGTGGGAAATCATGGTCGGCATCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGATCGCGCAGATGTATTCGGCGATCCAGACCGGCCAGGCCCACGGCATGATGACCCTCGACCAGCACCTG
CAGGACCTGGTCAAGCGCGGCCTGATCCTCAAGCCGCAGGCCCGCATGTACGCGAAGGACAAGCGTCTTTTCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas stutzeri DSM 10701

76.744

99.71

0.765
  pilT Pseudomonas aeruginosa PAK

77.286

98.261

0.759
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.213

95.362

0.67
  pilT Vibrio cholerae strain A1552

70.213

95.362

0.67
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Pseudomonas stutzeri DSM 10701

39.823

98.261

0.391
  pilU Acinetobacter baylyi ADP1

40.923

94.203

0.386
  pilU Vibrio cholerae strain A1552

39.692

94.203

0.374