Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H8B22_RS02910 Genome accession   NZ_CP060820
Coordinates   609351..610481 (-) Length   376 a.a.
NCBI ID   WP_187712630.1    Uniprot ID   -
Organism   Agrilutibacter terrestris strain II4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 604351..615481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8B22_RS02880 (H8B22_02880) - 604671..605579 (-) 909 WP_187712624.1 aspartate carbamoyltransferase catalytic subunit -
  H8B22_RS02885 (H8B22_02885) ruvX 605612..606073 (-) 462 WP_187712625.1 Holliday junction resolvase RuvX -
  H8B22_RS02890 (H8B22_02890) - 606087..606653 (-) 567 WP_187712626.1 YqgE/AlgH family protein -
  H8B22_RS02895 (H8B22_02895) - 606748..608475 (+) 1728 WP_187712627.1 dipeptidyl-peptidase 3 family protein -
  H8B22_RS02900 (H8B22_02900) - 608477..609046 (+) 570 WP_187712628.1 DNA-3-methyladenine glycosylase I -
  H8B22_RS02905 (H8B22_02905) - 609125..609274 (+) 150 WP_187712629.1 hypothetical protein -
  H8B22_RS02910 (H8B22_02910) pilU 609351..610481 (-) 1131 WP_187712630.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H8B22_RS02915 (H8B22_02915) pilT 610616..611653 (-) 1038 WP_187712631.1 type IV pilus twitching motility protein PilT Machinery gene
  H8B22_RS02920 (H8B22_02920) - 611800..612480 (+) 681 WP_407060823.1 YggS family pyridoxal phosphate-dependent enzyme -
  H8B22_RS02925 (H8B22_02925) proC 612558..613394 (+) 837 WP_187712632.1 pyrroline-5-carboxylate reductase -
  H8B22_RS02930 (H8B22_02930) - 613402..613893 (+) 492 WP_187712633.1 DUF4426 domain-containing protein -
  H8B22_RS02935 (H8B22_02935) - 613924..614721 (-) 798 WP_187712634.1 ABC transporter substrate-binding protein -
  H8B22_RS02940 (H8B22_02940) pdxH 614718..615320 (-) 603 WP_187712635.1 pyridoxamine 5'-phosphate oxidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41918.33 Da        Isoelectric Point: 6.8285

>NTDB_id=478605 H8B22_RS02910 WP_187712630.1 609351..610481(-) (pilU) [Agrilutibacter terrestris strain II4]
MSSIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQNPLTPQQSRDLVLNVMTPPQREEFEKTHECNFAIGVTG
VGRFRVSCFYQRNQVGMVLRRIETKIPTVEELSLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNMNSTGHIIT
IEDPIEFVHKHEGCIVTQREVGIDTDSWDNALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLVQDYIRDGEIHKLKEVMKESVQLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDAKTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=478605 H8B22_RS02910 WP_187712630.1 609351..610481(-) (pilU) [Agrilutibacter terrestris strain II4]
ATGAGCAGCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCGATGAAGGTCCACGGCAAGATCTCGCCGATCACGCAGAACCCGCTCACGCCGCAGCAGAGCCGCGACCTCG
TCCTCAACGTGATGACGCCGCCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGACCGGC
GTCGGCCGCTTCCGCGTGTCGTGCTTCTACCAGCGCAACCAGGTCGGCATGGTCCTGCGCCGCATCGAGACCAAGATCCC
GACGGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGAGGCATCATCATCTTCGTCGGCG
CGACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACATGAATTCGACCGGCCACATCATCACG
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAAGGCTGCATCGTCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGACAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCACGCGAACAATGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGACCTCTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGCTGATCCCGACGCCGGACGGCAAGGGCCGCCGCGTCGCGATGGAAATCCTGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGAATCCGTGCAGCTGGGCATGAAAACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCACTGCGTTACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCCAAGACCCTGTCGCAGGGCCTGGACGGCGTGGAAGTGGCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.392

97.606

0.668
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.667

98.936

0.412
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367