Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H9K76_RS02075 Genome accession   NZ_CP060714
Coordinates   428209..429252 (-) Length   347 a.a.
NCBI ID   WP_187597949.1    Uniprot ID   A0A7G9RQ29
Organism   Diaphorobacter ruginosibacter strain DSM 27467     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 423209..434252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9K76_RS02045 (H9K76_02045) - 423222..423557 (+) 336 WP_246475248.1 YraN family protein -
  H9K76_RS02050 (H9K76_02050) - 423682..424275 (+) 594 WP_187597944.1 SIS domain-containing protein -
  H9K76_RS02055 (H9K76_02055) - 424275..424871 (+) 597 Protein_410 BON domain-containing protein -
  H9K76_RS02060 (H9K76_02060) - 425215..426132 (-) 918 WP_187597946.1 NAD(P)-dependent oxidoreductase -
  H9K76_RS02065 (H9K76_02065) pilU 426257..427393 (-) 1137 WP_187597947.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H9K76_RS02070 (H9K76_02070) - 427501..428133 (-) 633 WP_187597948.1 cyclic nucleotide-binding domain-containing protein -
  H9K76_RS02075 (H9K76_02075) pilT 428209..429252 (-) 1044 WP_187597949.1 type IV pilus twitching motility protein PilT Machinery gene
  H9K76_RS02080 (H9K76_02080) - 429401..430123 (+) 723 WP_187597950.1 YggS family pyridoxal phosphate-dependent enzyme -
  H9K76_RS02085 (H9K76_02085) - 430238..431383 (+) 1146 WP_187597951.1 aminotransferase class V-fold PLP-dependent enzyme -
  H9K76_RS02090 (H9K76_02090) - 431448..431858 (+) 411 WP_187597952.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  H9K76_RS02095 (H9K76_02095) - 431999..433333 (+) 1335 WP_246475602.1 DUF445 family protein -
  H9K76_RS02100 (H9K76_02100) - 433390..433878 (-) 489 WP_246475250.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38217.00 Da        Isoelectric Point: 6.7743

>NTDB_id=477970 H9K76_RS02075 WP_187597949.1 428209..429252(-) (pilT) [Diaphorobacter ruginosibacter strain DSM 27467]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDSQRKTYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNNVGMQTLDQN
LTDLVRRNIISPAEARAKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=477970 H9K76_RS02075 WP_187597949.1 428209..429252(-) (pilT) [Diaphorobacter ruginosibacter strain DSM 27467]
GTGGACATTACCCAACTGCTTGCTTTCAGTGTCAAGAACAAAGCATCCGACCTGCACCTCTCGGCCGGCCTGCCGCCCAT
GATCCGGGTCCACGGCGACGTGCGCCGCATCAACGTGGACGCGCTCGACCACAAGACGGTGCACGCCATGGTGTACGACA
TCATGAGCGACTCGCAGCGCAAGACCTACGAGGAATTCCTGGAAGTCGACTTCTCCTTCGAGATCGAAGGCCTTGCGCGC
TTCCGCGTGAACGCGTTCAATCAGAACCGCGGCGCGGCAGCCGTGTTCCGTACGATTCCCAGCAAGATCCTGACGCTCGA
GCAGTTGAATGCGCCCAAGATCTTCGGCGACCTGGCACTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTCGCCGCGATGGTGAACTACCTGAACGAGACCGAGTACGGCCACATCCTGACCGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGCCCGATGACGCTGTCGTTCGCGGC
GGCATTGAAATCGGCGCTGCGTGAAGACCCGGATGCGATCCTGGTGGGCGAAATGCGCGACCTGGAAACGATCCGCCTTG
CGATGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGAGTGCCGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCCGCCGAGGAAAAGGAAATGGTCCGCGCGATGCTCTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGATGGCCAGGGCCGCGTTGCCGCGCATGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAACAATGTCGGCATGCAGACCCTGGACCAGAAC
TTGACCGATCTGGTGCGCCGCAACATCATCAGCCCCGCCGAGGCCCGCGCCAAGGCGAAGATTCCGGAGAATTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G9RQ29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.812

99.424

0.764
  pilT Acinetobacter baumannii strain A118

76.812

99.424

0.764
  pilT Acinetobacter nosocomialis M2

76.812

99.424

0.764
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.652

99.424

0.752
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403