Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H9K76_RS02065 Genome accession   NZ_CP060714
Coordinates   426257..427393 (-) Length   378 a.a.
NCBI ID   WP_187597947.1    Uniprot ID   A0A7G9RQ27
Organism   Diaphorobacter ruginosibacter strain DSM 27467     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 421257..432393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9K76_RS02035 (H9K76_02035) - 421805..422206 (+) 402 WP_187597941.1 hypothetical protein -
  H9K76_RS02040 (H9K76_02040) rsmI 422203..423132 (-) 930 WP_187597942.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  H9K76_RS02045 (H9K76_02045) - 423222..423557 (+) 336 WP_246475248.1 YraN family protein -
  H9K76_RS02050 (H9K76_02050) - 423682..424275 (+) 594 WP_187597944.1 SIS domain-containing protein -
  H9K76_RS02055 (H9K76_02055) - 424275..424871 (+) 597 Protein_410 BON domain-containing protein -
  H9K76_RS02060 (H9K76_02060) - 425215..426132 (-) 918 WP_187597946.1 NAD(P)-dependent oxidoreductase -
  H9K76_RS02065 (H9K76_02065) pilU 426257..427393 (-) 1137 WP_187597947.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H9K76_RS02070 (H9K76_02070) - 427501..428133 (-) 633 WP_187597948.1 cyclic nucleotide-binding domain-containing protein -
  H9K76_RS02075 (H9K76_02075) pilT 428209..429252 (-) 1044 WP_187597949.1 type IV pilus twitching motility protein PilT Machinery gene
  H9K76_RS02080 (H9K76_02080) - 429401..430123 (+) 723 WP_187597950.1 YggS family pyridoxal phosphate-dependent enzyme -
  H9K76_RS02085 (H9K76_02085) - 430238..431383 (+) 1146 WP_187597951.1 aminotransferase class V-fold PLP-dependent enzyme -
  H9K76_RS02090 (H9K76_02090) - 431448..431858 (+) 411 WP_187597952.1 FKBP-type peptidyl-prolyl cis-trans isomerase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41697.19 Da        Isoelectric Point: 6.9077

>NTDB_id=477969 H9K76_RS02065 WP_187597947.1 426257..427393(-) (pilU) [Diaphorobacter ruginosibacter strain DSM 27467]
MERDQASKFINDLLRLMVSRGGSDLFITAEFPPAIKVDGAVTKVSPQPLTPAHTLVLARSIMSDKQIADFERTKECNFAI
SPAGIGRFRVNAFVQQGRVGMVLRTIPLTLPTIDGLGVPQVLKDVTMTKRGLCILVGATGSGKSTTLAAMLDWRNLNSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTESWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRALVSQRLIPKQDGKGRAAAVEIMLNSPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQALFDLFEANVITYEDALRNADSVNDLRLQIKLSSQRAKTTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=477969 H9K76_RS02065 WP_187597947.1 426257..427393(-) (pilU) [Diaphorobacter ruginosibacter strain DSM 27467]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTTCGTCTGATGGTCAGCCGTGGCGGCAGCGACCTGTTCAT
CACGGCCGAGTTCCCACCCGCCATCAAGGTGGACGGCGCCGTGACCAAAGTATCGCCCCAGCCGCTGACGCCCGCGCACA
CGCTGGTTCTGGCCCGCTCGATCATGAGCGACAAGCAGATCGCCGACTTCGAGCGCACCAAGGAGTGCAATTTCGCGATC
TCGCCCGCGGGTATCGGCCGCTTCCGGGTGAATGCCTTCGTGCAGCAGGGACGCGTCGGCATGGTGCTGCGGACCATTCC
GCTCACGCTGCCCACCATCGACGGCCTGGGCGTGCCGCAGGTGCTCAAGGACGTGACGATGACCAAGCGCGGCCTGTGCA
TCCTGGTCGGTGCCACGGGCTCGGGCAAGTCCACCACGCTGGCCGCGATGCTCGACTGGCGCAACCTGAACTCCTTCGGG
CACATCATCACCGTCGAGGACCCGGTCGAGTTCGTGCACCCCCACAAGAACTGCGTGGTCACGCAGCGCGAGGTAGGCCT
CGACACCGAAAGCTGGGAAGCGGCACTCAAGAACACGCTGCGCCAGGCCCCCGACGTCATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCGGTCGCCTTCGCAGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGGGCACAGTTGCTGATGGACCTGTCGCTGAACCT
GCGCGCGCTCGTCTCGCAGCGCCTGATCCCCAAGCAGGACGGCAAGGGCCGGGCCGCCGCCGTGGAGATCATGCTGAACT
CGCCACTGATCGCCGACCTGATCTTCAAGGGCGAGGTCGCCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACTTTCGACCAGGCGCTGTTCGACCTGTTCGAGGCCAACGTCATCACCTATGAGGACGCCCTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCCTGCAGATCAAGCTGAGCAGCCAGCGCGCGAAGACCACCGACCTGTCGGCTGGCACCGAGC
ATTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G9RQ27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.183

92.328

0.593
  pilU Acinetobacter baylyi ADP1

56.983

94.709

0.54
  pilU Vibrio cholerae strain A1552

54.31

92.063

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Acinetobacter nosocomialis M2

45.045

88.095

0.397
  pilT Acinetobacter baumannii D1279779

45.045

88.095

0.397
  pilT Acinetobacter baumannii strain A118

45.045

88.095

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.62

89.153

0.389
  pilT Legionella pneumophila strain Lp02

43.844

88.095

0.386
  pilT Legionella pneumophila strain ERS1305867

43.844

88.095

0.386
  pilT Acinetobacter baylyi ADP1

43.902

86.772

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.566

87.831

0.365
  pilT Vibrio cholerae strain A1552

41.566

87.831

0.365