Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7F36_RS07340 Genome accession   NZ_CP060296
Coordinates   1546624..1547667 (-) Length   347 a.a.
NCBI ID   WP_187054056.1    Uniprot ID   A0A7G8W3S2
Organism   Variovorax sp. PAMC28562     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1541624..1552667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F36_RS07310 (H7F36_07310) rsmI 1541673..1542659 (-) 987 WP_187054050.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  H7F36_RS07315 (H7F36_07315) - 1542693..1543064 (+) 372 WP_187054051.1 YraN family protein -
  H7F36_RS07320 (H7F36_07320) - 1543113..1543721 (+) 609 WP_187054052.1 SIS domain-containing protein -
  H7F36_RS07325 (H7F36_07325) - 1543718..1544491 (+) 774 WP_187054053.1 BON domain-containing protein -
  H7F36_RS07330 (H7F36_07330) - 1544509..1545432 (-) 924 WP_187054054.1 NAD(P)-dependent oxidoreductase -
  H7F36_RS07335 (H7F36_07335) pilU 1545456..1546592 (-) 1137 WP_187054055.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7F36_RS07340 (H7F36_07340) pilT 1546624..1547667 (-) 1044 WP_187054056.1 type IV pilus twitching motility protein PilT Machinery gene
  H7F36_RS07345 (H7F36_07345) - 1547715..1548401 (+) 687 WP_187054057.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7F36_RS07350 (H7F36_07350) - 1548503..1549777 (+) 1275 WP_187054058.1 Glu/Leu/Phe/Val family dehydrogenase -
  H7F36_RS07355 (H7F36_07355) - 1549862..1550506 (+) 645 WP_187054059.1 DUF2242 domain-containing protein -
  H7F36_RS07360 (H7F36_07360) - 1550523..1550942 (-) 420 WP_222620445.1 response regulator -
  H7F36_RS07365 (H7F36_07365) rocF 1551059..1551970 (-) 912 WP_187054061.1 arginase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38066.74 Da        Isoelectric Point: 6.8884

>NTDB_id=475540 H7F36_RS07340 WP_187054056.1 1546624..1547667(-) (pilT) [Variovorax sp. PAMC28562]
MDITQLLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQSRGAAAVFRTIPSKILSLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKDMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREGKVAQMYSSIQTGSGQGMQTLDQN
LTDLVRRSVISSAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=475540 H7F36_RS07340 WP_187054056.1 1546624..1547667(-) (pilT) [Variovorax sp. PAMC28562]
GTGGACATTACCCAACTGCTGGCATTCAGCGTCAAAAACAAGGCTTCGGACTTGCATCTCTCGTCTGGCTTGCCGCCGAT
GATCCGGGTGCATGGTGACGTGAGGCGCATCAATGTCGACGCGCTCGATCACAAGGCGGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAACACTACGAAGAATTTCTGGAAGTCGACTTCTCTTTCGAGATCGATGGCCTGGCCCGC
TTTCGGGTGAATGCCTTCAACCAGTCGCGCGGCGCTGCGGCGGTATTCCGGACCATTCCTTCGAAGATCCTGTCGCTGGA
GCAACTCAACGCGCCCAAGATCTTCGGCGAACTCGCATTGAAGCCGCGCGGCCTGGTGCTGGTGACAGGCCCGACCGGCT
CGGGCAAATCGACCACGCTCGCCGCGATGGTCAATTACCTGAACGAAACCGAGTACGGCCACATCCTGACGGTAGAAGAC
CCGATCGAATTCGTCCACGAATCGAAAAAGTGCCTGATCAATCAGCGCGAAGTCGGGCCGATGACGCTGTCTTTCGCGGC
CGCTCTGCGCTCTGCCCTGCGTGAAGATCCTGATGCGATTCTGGTCGGCGAAATGCGCGACCTGGAAACGATTCGCCTGG
CCATGACGGCGGCTGAAACGGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAAAAAGACATGATCCGCGCCATGCTGTCGGAGTCGCTTCAAGCGGTGATTTCTCAGAC
ACTGTGCAAGACCAAGGACGGCCAGGGCCGTGTTGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATTCGCAATCTGA
TCCGCGAGGGCAAGGTTGCGCAGATGTACTCGTCGATTCAAACTGGCAGCGGTCAGGGCATGCAGACGCTCGACCAGAAC
CTGACCGACCTGGTGCGCCGCAGCGTGATTTCATCTGCCGAGGCACGCGGCAAAGCCAAGATCCCCGAGAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8W3S2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.742

98.271

0.666
  pilT Vibrio cholerae strain A1552

67.742

98.271

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389