Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7F36_RS07335 Genome accession   NZ_CP060296
Coordinates   1545456..1546592 (-) Length   378 a.a.
NCBI ID   WP_187054055.1    Uniprot ID   -
Organism   Variovorax sp. PAMC28562     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1540456..1551592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F36_RS07300 (H7F36_07300) - 1540503..1540802 (+) 300 WP_315971447.1 DUF599 family protein -
  H7F36_RS07305 (H7F36_07305) - 1540948..1541676 (+) 729 WP_187054049.1 glutathione S-transferase -
  H7F36_RS07310 (H7F36_07310) rsmI 1541673..1542659 (-) 987 WP_187054050.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  H7F36_RS07315 (H7F36_07315) - 1542693..1543064 (+) 372 WP_187054051.1 YraN family protein -
  H7F36_RS07320 (H7F36_07320) - 1543113..1543721 (+) 609 WP_187054052.1 SIS domain-containing protein -
  H7F36_RS07325 (H7F36_07325) - 1543718..1544491 (+) 774 WP_187054053.1 BON domain-containing protein -
  H7F36_RS07330 (H7F36_07330) - 1544509..1545432 (-) 924 WP_187054054.1 NAD(P)-dependent oxidoreductase -
  H7F36_RS07335 (H7F36_07335) pilU 1545456..1546592 (-) 1137 WP_187054055.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7F36_RS07340 (H7F36_07340) pilT 1546624..1547667 (-) 1044 WP_187054056.1 type IV pilus twitching motility protein PilT Machinery gene
  H7F36_RS07345 (H7F36_07345) - 1547715..1548401 (+) 687 WP_187054057.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7F36_RS07350 (H7F36_07350) - 1548503..1549777 (+) 1275 WP_187054058.1 Glu/Leu/Phe/Val family dehydrogenase -
  H7F36_RS07355 (H7F36_07355) - 1549862..1550506 (+) 645 WP_187054059.1 DUF2242 domain-containing protein -
  H7F36_RS07360 (H7F36_07360) - 1550523..1550942 (-) 420 WP_222620445.1 response regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41893.98 Da        Isoelectric Point: 6.5059

>NTDB_id=475539 H7F36_RS07335 WP_187054055.1 1545456..1546592(-) (pilU) [Variovorax sp. PAMC28562]
MERDQASQFINELLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSGQALGSQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGVGRFRVNAFVQQGKVGMVMRTIPAKLPTIDGLGMPQVLKDVAMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRNQLLMDLSLNLRSLVSQRLIPTEDGQGRIAAVEILLNTPLISDMIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFENNAITFDDAIRNADSANDLRLQIKLNSRRAKSQDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=475539 H7F36_RS07335 WP_187054055.1 1545456..1546592(-) (pilU) [Variovorax sp. PAMC28562]
ATGGAACGCGATCAGGCCAGCCAGTTCATCAACGAACTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACTTGTTCAT
CACCGCCGACTTTCCGCCGGCCATCAAGGTCGACGGCAAGGTGACCAAGGTGTCGGGCCAGGCGCTCGGTTCTCAGCACA
CGCTGGCGCTCACCCGATCGGTCATGAACGACCGCCAGACGGCCGAGTTCGAGCGGACCAAGGAATGCAACTTCGCAATC
TCGCCGACCGGTGTCGGGCGCTTTCGCGTCAACGCATTCGTGCAGCAGGGCAAGGTCGGCATGGTGATGCGGACCATTCC
CGCCAAGCTTCCGACCATCGACGGTCTCGGTATGCCGCAGGTGCTCAAAGACGTGGCGATGACCAAGCGCGGCCTGACCA
TCCTGGTCGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCCGCCATGATCGACTGGCGCAATGAAAATTCATACGGC
CACATCGTGACCGTCGAAGACCCGGTCGAGTTCGTGCACCCGCACAAAAACTGCGTGGTAACGCAGCGCGAAGTGGGCAT
AGACACCGACAGCTGGGAGGCCGCTCTCAAGAACACGCTGCGCCAGGCGCCCGACGTCATTTTGATGGGCGAAATCCGCG
ATCGCGAGACGATGGAGCATGCCGTCGCATTCGCCGAAACCGGTCACCTTTGCATGGCGACACTGCATGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAATTTCTTTCCGGAAGAGCGCCGCAACCAGTTGTTGATGGACTTGTCCCTGAACCT
GCGCTCCCTGGTGTCGCAGCGCCTGATTCCGACCGAAGACGGCCAGGGGCGCATCGCAGCGGTCGAGATTTTGCTCAACA
CGCCCCTGATCTCCGACATGATCTTCAAGGGCGAAGTCGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACTTTCGACCAGGCGCTGTTCGATCTGTTCGAGAACAACGCGATCACTTTCGACGACGCCATTCGCAATGCCGA
TTCGGCGAACGACCTGCGTCTGCAGATCAAGCTGAACAGCCGACGCGCCAAGTCGCAAGATCTGTCTGCCGGCACCGAGC
ATTTCGCCATAGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.324

92.328

0.585
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

54.31

92.063

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baumannii strain A118

44.073

87.037

0.384
  pilT Acinetobacter nosocomialis M2

44.073

87.037

0.384
  pilT Legionella pneumophila strain Lp02

44.073

87.037

0.384
  pilT Legionella pneumophila strain ERS1305867

44.073

87.037

0.384
  pilT Acinetobacter baumannii D1279779

44.073

87.037

0.384
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362