Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7F35_RS08705 Genome accession   NZ_CP060295
Coordinates   1820082..1821125 (+) Length   347 a.a.
NCBI ID   WP_093247214.1    Uniprot ID   A0A3N7BFW7
Organism   Variovorax sp. PAMC26660     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1815082..1826125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F35_RS08670 (H7F35_08670) - 1815144..1815650 (-) 507 WP_187112503.1 ADP-ribosylglycohydrolase family protein -
  H7F35_RS08675 (H7F35_08675) - 1815676..1816197 (-) 522 WP_187112504.1 alpha-ketoglutarate-dependent dioxygenase AlkB family protein -
  H7F35_RS08680 (H7F35_08680) - 1816250..1817521 (-) 1272 WP_187112505.1 Glu/Leu/Phe/Val family dehydrogenase -
  H7F35_RS08685 (H7F35_08685) - 1817762..1818094 (+) 333 WP_187112506.1 DUF4148 domain-containing protein -
  H7F35_RS08690 (H7F35_08690) - 1818248..1818706 (+) 459 WP_187112507.1 hypothetical protein -
  H7F35_RS08695 (H7F35_08695) - 1818842..1819309 (+) 468 WP_187112508.1 Lrp/AsnC ligand binding domain-containing protein -
  H7F35_RS08700 (H7F35_08700) - 1819329..1820030 (-) 702 WP_187112509.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7F35_RS08705 (H7F35_08705) pilT 1820082..1821125 (+) 1044 WP_093247214.1 type IV pilus twitching motility protein PilT Machinery gene
  H7F35_RS08710 (H7F35_08710) pilU 1821150..1822286 (+) 1137 WP_187112510.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7F35_RS08715 (H7F35_08715) - 1822296..1823846 (-) 1551 WP_187112511.1 MFS transporter -
  H7F35_RS08720 (H7F35_08720) - 1823905..1824387 (-) 483 WP_187112512.1 MerR family transcriptional regulator -
  H7F35_RS08725 (H7F35_08725) - 1824475..1825383 (+) 909 WP_187112513.1 NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38120.78 Da        Isoelectric Point: 6.8354

>NTDB_id=475519 H7F35_RS08705 WP_093247214.1 1820082..1821125(+) (pilT) [Variovorax sp. PAMC26660]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTDLVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=475519 H7F35_RS08705 WP_093247214.1 1820082..1821125(+) (pilT) [Variovorax sp. PAMC26660]
GTGGACATTACCCAATTGCTGGCGTTCAGCGTCAAGAACAAAGCGTCCGACTTGCACTTGTCGGCCGGCCTTCCGCCCAT
GATCCGCGTCAACGGCGACGTGCGCCGTATCAACGTCGATGCGCTCGATCACAAGGGCGTGCACGCGATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTTCGCGTGAACGCCTTCAACCAGGCGCGTGGCGCGGCCGCGGTGTTCCGGACCATTCCCTCGAAGATCCTGACGCTGGA
GCAACTGAATGCGCCGAAGATTTTCGGCGAACTGGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACGGGCCCCACGGGTT
CGGGCAAGTCGACCACGCTGGCCGCGATGGTGAACTACCTCAACGAAAACGAATACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGATGACGCTGTCGTTCTCGAA
CGCGCTGCGCTCCGCCCTGCGCGAAGACCCGGACGCCATTCTTGTGGGCGAGTTGCGCGACCTGGAAACCATTCGCCTGG
CGATGACCGCGGCCGAAACGGGCCATTTGGTGTTCGGCACACTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAAAAGGAAATGATCCGCGCGATGCTGTCCGAGTCGTTGCAGGCGGTGATCTCGCAAAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAATTTGA
TCCGCGAAGCCAAGGTGGCACAGATGTATTCCACCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTCGACCAGAAC
CTGACGGACCTGGTGCGTCGCAATACGATCTCTGCTGCAGAAGCCCGCGGCAAAGCCAAGATCCCAGAGAATTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3N7BFW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398