Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7F35_RS08710 Genome accession   NZ_CP060295
Coordinates   1821150..1822286 (+) Length   378 a.a.
NCBI ID   WP_187112510.1    Uniprot ID   -
Organism   Variovorax sp. PAMC26660     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1816150..1827286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F35_RS08680 (H7F35_08680) - 1816250..1817521 (-) 1272 WP_187112505.1 Glu/Leu/Phe/Val family dehydrogenase -
  H7F35_RS08685 (H7F35_08685) - 1817762..1818094 (+) 333 WP_187112506.1 DUF4148 domain-containing protein -
  H7F35_RS08690 (H7F35_08690) - 1818248..1818706 (+) 459 WP_187112507.1 hypothetical protein -
  H7F35_RS08695 (H7F35_08695) - 1818842..1819309 (+) 468 WP_187112508.1 Lrp/AsnC ligand binding domain-containing protein -
  H7F35_RS08700 (H7F35_08700) - 1819329..1820030 (-) 702 WP_187112509.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7F35_RS08705 (H7F35_08705) pilT 1820082..1821125 (+) 1044 WP_093247214.1 type IV pilus twitching motility protein PilT Machinery gene
  H7F35_RS08710 (H7F35_08710) pilU 1821150..1822286 (+) 1137 WP_187112510.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7F35_RS08715 (H7F35_08715) - 1822296..1823846 (-) 1551 WP_187112511.1 MFS transporter -
  H7F35_RS08720 (H7F35_08720) - 1823905..1824387 (-) 483 WP_187112512.1 MerR family transcriptional regulator -
  H7F35_RS08725 (H7F35_08725) - 1824475..1825383 (+) 909 WP_187112513.1 NAD(P)-dependent oxidoreductase -
  H7F35_RS08730 (H7F35_08730) - 1825535..1826329 (-) 795 WP_187112514.1 BON domain-containing protein -
  H7F35_RS08735 (H7F35_08735) - 1826326..1826970 (-) 645 WP_187112515.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41794.88 Da        Isoelectric Point: 6.4172

>NTDB_id=475520 H7F35_RS08710 WP_187112510.1 1821150..1822286(+) (pilU) [Variovorax sp. PAMC26660]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQVAEFERTKECNFAI
SPTGVGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVAMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRTQLLMDLSLNLRSLVSQRLVPTEDGQGRVAAVEVLLNTPLIADLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSITFDDAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=475520 H7F35_RS08710 WP_187112510.1 1821150..1822286(+) (pilU) [Variovorax sp. PAMC26660]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTTCCGCCGGCCATCAAGGTTGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGCGCGCAGCACA
CGCTGGCGCTGACGCGTTCGGTCATGAACGATCGCCAGGTGGCGGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCGACCGGCGTGGGGCGCTTTCGGGTCAATGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGATGGCCTGGGCATGCCGCAGGTGCTGAAAGACGTGGCGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCCATGGTCGACTGGCGCAACGAGAACTCCTTCGGC
CACATCGTGACGGTAGAAGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCACCCGACGTGATTTTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCGGTGGCCTTCGCCGAAACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAATTTCTTCCCCGAAGAGCGCCGCACGCAACTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTCTCGCAGCGGCTGGTACCCACCGAAGACGGCCAGGGCCGCGTGGCCGCGGTCGAAGTGCTGCTGAACA
CGCCGCTGATTGCCGACCTGATCTTCAAGGGCGAAGTGGGCGAGATCAAGGAGATCATGCGCAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAGCCACTCGATCACCTTCGACGACGCGATCCGCAACGCCGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGTGCGCGCAGCACCGACCTGGCTGCCGGCACCGAGC
ACTTCGCGATCGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

57.263

94.709

0.542
  pilU Vibrio cholerae strain A1552

53.444

96.032

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.291

89.153

0.413
  pilT Pseudomonas aeruginosa PAK

43.917

89.153

0.392
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Legionella pneumophila strain Lp02

43.161

87.037

0.376
  pilT Legionella pneumophila strain ERS1305867

43.161

87.037

0.376
  pilT Acinetobacter baumannii strain A118

44.514

84.392

0.376
  pilT Acinetobacter nosocomialis M2

44.514

84.392

0.376
  pilT Acinetobacter baumannii D1279779

44.514

84.392

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.533

83.862

0.365
  pilT Vibrio cholerae strain A1552

43.533

83.862

0.365
  pilT Acinetobacter baylyi ADP1

42.724

85.45

0.365