Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7683_RS07305 Genome accession   NZ_CP060288
Coordinates   1553311..1554345 (-) Length   344 a.a.
NCBI ID   WP_013717519.1    Uniprot ID   A0A379IR40
Organism   Pseudomonas mendocina strain CPS5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1548311..1559345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7683_RS07280 (H7683_07280) - 1548460..1549104 (-) 645 WP_209450620.1 DUF2076 domain-containing protein -
  H7683_RS07285 (H7683_07285) - 1549215..1549613 (-) 399 WP_209450621.1 TM2 domain-containing protein -
  H7683_RS07290 (H7683_07290) - 1549761..1550972 (+) 1212 WP_013717516.1 putative DNA modification/repair radical SAM protein -
  H7683_RS07295 (H7683_07295) - 1550972..1551832 (+) 861 WP_013717517.1 TIGR03915 family putative DNA repair protein -
  H7683_RS07300 (H7683_07300) pilU 1552079..1553224 (-) 1146 WP_013717518.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7683_RS07305 (H7683_07305) pilT 1553311..1554345 (-) 1035 WP_013717519.1 type IV pilus twitching motility protein PilT Machinery gene
  H7683_RS07310 (H7683_07310) - 1554421..1555113 (+) 693 WP_209450622.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7683_RS07315 (H7683_07315) proC 1555131..1555949 (+) 819 WP_013717521.1 pyrroline-5-carboxylate reductase -
  H7683_RS07320 (H7683_07320) - 1555961..1556554 (+) 594 WP_209450623.1 YggT family protein -
  H7683_RS07325 (H7683_07325) - 1556557..1556847 (+) 291 WP_013717523.1 DUF167 domain-containing protein -
  H7683_RS07330 (H7683_07330) - 1557029..1559005 (+) 1977 WP_209450624.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37935.60 Da        Isoelectric Point: 6.8887

>NTDB_id=475370 H7683_RS07305 WP_013717519.1 1553311..1554345(-) (pilT) [Pseudomonas mendocina strain CPS5]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDACL
KTLVSKGLVSRDSAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=475370 H7683_RS07305 WP_013717519.1 1553311..1554345(-) (pilT) [Pseudomonas mendocina strain CPS5]
ATGGATATCACTGAGCTGCTCGCCTTCAGCGCCAAGCAAGGCGCGTCGGATTTGCACCTCTCGGCGGGGCTGCCGCCGAT
GATCCGCGTTGACGGCGATGTGCGCCGCATCAACCTGCCGCCGCTGGATCACAAGCAGGTTCACGCGCTGATCTACGACA
TCATGAATGACAAGCAGCGCAAGGATTACGAAGAGTTTCTCGAGACCGACTTCTCCTTCGAAGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCCTTTAATCAGAACCGTGGTGCCGGTGCGGTGTTCCGTACCATTCCGTCCAAGGTACTGACCATGGA
CGACCTGGGCATGGGCGAGGTATTCCGCAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGCCCTACCGGTT
CGGGTAAGTCGACTACCCTGGCGGCGATGCTCGATTACCTCAACAGCAACAAGTACCACCATATCCTCACCATCGAAGAC
CCCATCGAATTCGTTCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAGGTGCACCGCGACACCCACGGCTTCTCCGA
AGCGCTGCGTTCGGCGCTGCGTGAAGACCCGGACATCATCCTGGTGGGTGAGATGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCAGCGGAAACCGGCCACCTCGTGTTCGGCACCCTGCACACCACCTCCGCTGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAAGAGAAGTCGATGGTGCGTTCGATGCTCTCCGAGTCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGATCGGTGGCGGTCGCGTGGCCGCGCACGAAATCATGATCGGTACGCCTGCCATTCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCGGCGCGCTGGGCATGCAGACCCTCGATGCGTGTCTC
AAGACCCTGGTATCCAAGGGCCTGGTCAGCCGCGACAGTGCGCGCGAGAAGGCCAAGAGCCCCGAGAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A379IR40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

94.767

100

0.948
  pilT Pseudomonas aeruginosa PAK

93.605

100

0.936
  pilT Acinetobacter nosocomialis M2

84.884

100

0.849
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

75.148

98.256

0.738
  pilT Legionella pneumophila strain ERS1305867

75.148

98.256

0.738
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.401
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398