Detailed information NTG0475369

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H7683_RS07300
• Genome accession: NZ_CP060288
• Coordinates: 1552079..1553224 (-)
• Length: 381 a.a.
• NCBI ID: WP_013717518.1
• Uniprot ID: -
• Organism: Pseudomonas mendocina strain CPS5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1547079..1558224

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7683_RS07280 (H7683_07280)	-	1548460..1549104 (-)	645	WP_209450620.1	DUF2076 domain-containing protein	-
H7683_RS07285 (H7683_07285)	-	1549215..1549613 (-)	399	WP_209450621.1	TM2 domain-containing protein	-
H7683_RS07290 (H7683_07290)	-	1549761..1550972 (+)	1212	WP_013717516.1	putative DNA modification/repair radical SAM protein	-
H7683_RS07295 (H7683_07295)	-	1550972..1551832 (+)	861	WP_013717517.1	TIGR03915 family putative DNA repair protein	-
H7683_RS07300 (H7683_07300)	pilU	1552079..1553224 (-)	1146	WP_013717518.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H7683_RS07305 (H7683_07305)	pilT	1553311..1554345 (-)	1035	WP_013717519.1	type IV pilus twitching motility protein PilT	Machinery gene
H7683_RS07310 (H7683_07310)	-	1554421..1555113 (+)	693	WP_209450622.1	YggS family pyridoxal phosphate-dependent enzyme	-
H7683_RS07315 (H7683_07315)	proC	1555131..1555949 (+)	819	WP_013717521.1	pyrroline-5-carboxylate reductase	-
H7683_RS07320 (H7683_07320)	-	1555961..1556554 (+)	594	WP_209450623.1	YggT family protein	-
H7683_RS07325 (H7683_07325)	-	1556557..1556847 (+)	291	WP_013717523.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42469.79 Da
• Isoelectric Point: 6.5931

>NTDB_id=475369 H7683_RS07300 WP_013717518.1 1552079..1553224(-) (pilU) [Pseudomonas mendocina strain CPS5]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNAMSPEMTRETVLGVMNEAQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETTIPTIDELKLPDILKKLSLTKRGLVLFVGATGTGKSTSLAAMIGYRNRNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVIMIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSNEQGMQTFDQA
LYNLYTQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMAQGLALEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=475369 H7683_RS07300 WP_013717518.1 1552079..1553224(-) (pilU) [Pseudomonas mendocina strain CPS5]
ATGGAATTCGAAAAACTACTGCGCCTGATGGTCGAAAAAGGCGGTTCCGACCTGTTCATCACCGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCCGTGACCAAGAACGCCATGTCGCCGGAGATGACCCGCGAAACCGTGCTGGGTG
TGATGAACGAGGCGCAGCGCCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGTGGCATCGGTCGC
TTCCGTGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGTCGTATCGAGACCACCATCCCGACCATCGA
TGAGCTGAAGCTGCCGGACATCCTCAAGAAACTGTCATTGACCAAGCGCGGCCTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGTAAATCCACCTCGCTGGCGGCGATGATCGGCTACCGTAACCGCAATAGCAGCGGCCACATCATCTCCATCGAAGAC
CCTATCGAATACATCCACCAGCACCAGAGCTGCATCGTCACGCAGCGCGAAGTGGGTATCGACACCGAGTCGTTCGAGGT
CGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATTGGCGAGGTACGGACCCGCGAGACCATGGATCACG
CCGTGGCCTTCGCCGAAACCGGGCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGTTGGACCGCATC
ATCAACTTCTTCCCAGCCGACCGGCAGAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAGGCTATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGATGGCAAGGGACGTCGCGCGGTGATCGAGGTGCTGATCAATACCCCGCTGGCTGCCGATCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGGCCTGATGAAGCGCTCCAACGAGCAGGGCATGCAGACCTTCGACCAGGCC
TTGTACAACCTCTATACCCAGGGTGAGATCACCTACGAAGACGCGCTGCTCTACGCCGATTCGGCCAACGATCTGCGTCT
GATGATCAAGCTCGGCTCGGAAACCGATGGCGATCACCTGACCAGCATGGCCCAGGGCCTGGCGCTGGAGGTCAGCGAGG
AAGATCCGGGGCGGCGCTTTCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	91.339	100	0.913
pilU	Acinetobacter baylyi ADP1	63.585	93.701	0.596
pilU	Vibrio cholerae strain A1552	58.286	91.864	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.452	88.189	0.383