Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6K11_RS10135 Genome accession   NZ_CP070134
Coordinates   1883840..1884820 (-) Length   326 a.a.
NCBI ID   WP_072023908.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1281     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1878840..1889820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K11_RS10105 (I6K11_10115) yggI 1879564..1880061 (+) 498 WP_032152710.1 SprT family zinc-dependent metalloprotease -
  I6K11_RS10110 (I6K11_10120) endA 1880156..1880863 (+) 708 WP_000286497.1 deoxyribonuclease I -
  I6K11_RS10115 (I6K11_10125) rsmE 1880943..1881674 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  I6K11_RS10120 (I6K11_10130) gshB 1881687..1882637 (+) 951 WP_000593273.1 glutathione synthase -
  I6K11_RS10125 (I6K11_10135) yqgE 1882746..1883309 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  I6K11_RS10130 (I6K11_10140) ruvX 1883309..1883725 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  I6K11_RS10135 (I6K11_10145) pilT 1883840..1884820 (-) 981 WP_072023908.1 type IV pilus twitching motility protein PilT Machinery gene
  I6K11_RS10140 (I6K11_10150) yggS 1884838..1885542 (+) 705 WP_032152711.1 pyridoxal phosphate homeostasis protein -
  I6K11_RS10145 (I6K11_10155) yggT 1885560..1886126 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  I6K11_RS10150 (I6K11_10160) yggU 1886123..1886413 (+) 291 WP_001277229.1 DUF167 family protein YggU -
  I6K11_RS10155 (I6K11_10165) rdgB 1886421..1887014 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  I6K11_RS10160 (I6K11_10170) hemW 1887007..1888143 (+) 1137 WP_032152712.1 radical SAM family heme chaperone HemW -
  I6K11_RS10165 (I6K11_10175) yggM 1888212..1889219 (-) 1008 WP_032152713.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35958.12 Da        Isoelectric Point: 5.5860

>NTDB_id=473040 I6K11_RS10135 WP_072023908.1 1883840..1884820(-) (pilT) [Escherichia coli strain FDAARGOS_1281]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQW
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=473040 I6K11_RS10135 WP_072023908.1 1883840..1884820(-) (pilT) [Escherichia coli strain FDAARGOS_1281]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCGTTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTAACAG
CGGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCAGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGTGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.037

100

0.38
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362