Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   H2O77_RS03725 Genome accession   NZ_CP059843
Coordinates   877005..877445 (+) Length   146 a.a.
NCBI ID   WP_215823931.1    Uniprot ID   -
Organism   Cobetia sp. 4B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 872005..882445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2O77_RS03710 (H2O77_03705) pilD 872329..873189 (-) 861 WP_240548701.1 A24 family peptidase Machinery gene
  H2O77_RS03715 (H2O77_03710) pilC 873281..874519 (-) 1239 WP_200019032.1 type II secretion system F family protein Machinery gene
  H2O77_RS03720 (H2O77_03715) pilB 874695..876431 (-) 1737 WP_215823930.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H2O77_RS03725 (H2O77_03720) pilA2 877005..877445 (+) 441 WP_215823931.1 pilin Machinery gene
  H2O77_RS03730 (H2O77_03725) - 877887..879281 (+) 1395 WP_215823932.1 hypothetical protein -
  H2O77_RS03735 (H2O77_03730) - 879332..879634 (-) 303 WP_043332474.1 hypothetical protein -
  H2O77_RS03740 (H2O77_03735) - 879804..881090 (-) 1287 WP_215823933.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14976.78 Da        Isoelectric Point: 4.3148

>NTDB_id=472189 H2O77_RS03725 WP_215823931.1 877005..877445(+) (pilA2) [Cobetia sp. 4B]
MTRKQQAGFTLIELMIVIAIIGILAAIAIPRYQDYTKRANVSEGLQLAAAAKTAVTEVYSTSSSFPATNAAAGLQEASTI
NGNAVDSVTVGANGVITVNYNGTVDDNAELTLTPTASATGGSITWSCGKNATMDDAWVPADCRSGS

Nucleotide


Download         Length: 441 bp        

>NTDB_id=472189 H2O77_RS03725 WP_215823931.1 877005..877445(+) (pilA2) [Cobetia sp. 4B]
ATGACACGCAAGCAACAAGCTGGTTTTACACTTATCGAGCTGATGATCGTTATTGCCATCATTGGTATTTTGGCTGCTAT
AGCAATCCCGCGTTACCAGGATTATACTAAGCGTGCTAATGTCTCTGAAGGTTTACAGTTGGCAGCAGCAGCAAAAACAG
CAGTTACTGAAGTTTACTCGACCTCAAGTTCATTTCCTGCAACTAATGCAGCTGCCGGACTTCAGGAGGCCAGCACTATT
AACGGAAATGCGGTTGACTCTGTTACTGTTGGCGCAAACGGTGTTATCACGGTCAATTATAATGGTACCGTAGATGATAA
TGCTGAACTGACGCTTACACCCACGGCTTCTGCAACTGGTGGCAGTATTACTTGGAGTTGTGGTAAAAATGCAACCATGG
ATGATGCATGGGTGCCTGCAGACTGTCGTTCTGGTTCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

52.857

95.89

0.507
  pilA2 Legionella pneumophila strain ERS1305867

52.857

95.89

0.507
  comP Acinetobacter baylyi ADP1

48.299

100

0.486
  pilA Ralstonia pseudosolanacearum GMI1000

41.52

100

0.486
  pilE Neisseria gonorrhoeae strain FA1090

43.671

100

0.473
  pilE Neisseria gonorrhoeae MS11

45.139

98.63

0.445
  pilA Haemophilus influenzae 86-028NP

47.445

93.836

0.445
  pilA/pilA1 Eikenella corrodens VA1

41.29

100

0.438
  pilA Haemophilus influenzae Rd KW20

44.928

94.521

0.425
  pilA Vibrio cholerae C6706

38.411

100

0.397
  pilA Vibrio cholerae strain A1552

38.411

100

0.397
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.411

100

0.397
  pilA Vibrio parahaemolyticus RIMD 2210633

42.52

86.986

0.37
  pilA Vibrio campbellii strain DS40M4

38.129

95.205

0.363