Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H2O77_RS03720 Genome accession   NZ_CP059843
Coordinates   874695..876431 (-) Length   578 a.a.
NCBI ID   WP_215823930.1    Uniprot ID   -
Organism   Cobetia sp. 4B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 869695..881431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2O77_RS03695 (H2O77_03690) - 870359..871312 (+) 954 WP_043332483.1 Nudix family hydrolase -
  H2O77_RS03700 (H2O77_03695) yacG 871444..871689 (-) 246 WP_153635187.1 DNA gyrase inhibitor YacG -
  H2O77_RS03705 (H2O77_03700) coaE 871682..872332 (-) 651 WP_215823929.1 dephospho-CoA kinase -
  H2O77_RS03710 (H2O77_03705) pilD 872329..873189 (-) 861 WP_240548701.1 A24 family peptidase Machinery gene
  H2O77_RS03715 (H2O77_03710) pilC 873281..874519 (-) 1239 WP_200019032.1 type II secretion system F family protein Machinery gene
  H2O77_RS03720 (H2O77_03715) pilB 874695..876431 (-) 1737 WP_215823930.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H2O77_RS03725 (H2O77_03720) pilA2 877005..877445 (+) 441 WP_215823931.1 pilin Machinery gene
  H2O77_RS03730 (H2O77_03725) - 877887..879281 (+) 1395 WP_215823932.1 hypothetical protein -
  H2O77_RS03735 (H2O77_03730) - 879332..879634 (-) 303 WP_043332474.1 hypothetical protein -
  H2O77_RS03740 (H2O77_03735) - 879804..881090 (-) 1287 WP_215823933.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63514.53 Da        Isoelectric Point: 4.7991

>NTDB_id=472188 H2O77_RS03720 WP_215823930.1 874695..876431(-) (pilB) [Cobetia sp. 4B]
MPQSEHTLKGFAARLVDSGLLEPQAAQLAEQEANEAGESLLLHIIAHGLVTARDATLAAAWEYGLPYVDLDALRIDSLPS
IDELPEKVLRKLGVLPLYRREHRLTVGVPSPSTLTHLDELQFATGLSIESVLVAADQLTPALEQYLEAGEESAMDMLSED
GEALDGLDFEGEEDNNNDLAITANSDDAPVVRFVHKVLLDAIKRGASDIHFEPYESTYRIRFRIDGMLVENTRPPFNMRN
RISARLKVMSRMDISERRLPQDGAIKLRLSKSRSIDFRVNTLPTVYGEKLVLRILDPSSAKMGIEALGFEPEQRAMFEKT
LDQPQGMILVTGPTGSGKTVTLYTGLNILNTPERNISTAEDPVELKVEGVNQVNVLPKIGLDFASALRAFLRQDPDVVMV
GEIRDKETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLSNMGIAPFNIASSVSLIIAQRLARRLCTHCRQPTEIPPEAMA
SMGFSEDNGFSAAELEAATIFRPVGCSQCTLGYKGRVGIYEVMPISDEMSRLIMREGTAIELADQARKEGLSDLRRSGLR
KVMSGMTSLEEVNRITKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=472188 H2O77_RS03720 WP_215823930.1 874695..876431(-) (pilB) [Cobetia sp. 4B]
ATGCCCCAGTCAGAACACACGCTAAAAGGATTTGCCGCCCGACTGGTCGACTCTGGCCTGCTCGAACCACAGGCAGCACA
GCTGGCTGAACAGGAAGCCAATGAGGCCGGGGAAAGCCTGTTGCTGCACATCATTGCGCATGGTCTGGTGACAGCACGTG
ACGCGACATTGGCGGCCGCCTGGGAATATGGCCTGCCGTATGTGGATCTCGATGCCCTTCGTATCGACTCGCTGCCTTCC
ATTGATGAACTGCCCGAGAAGGTCCTGCGCAAGTTGGGTGTACTGCCACTCTATCGCCGTGAGCATCGGCTGACCGTCGG
CGTGCCCTCCCCATCAACACTTACGCATCTAGATGAGCTGCAATTTGCCACCGGCTTGTCGATCGAGAGTGTCCTGGTCG
CCGCCGACCAGCTGACGCCGGCATTGGAGCAGTACCTGGAAGCCGGCGAAGAAAGCGCCATGGACATGCTGAGCGAAGAT
GGCGAAGCACTCGATGGGCTAGATTTTGAAGGCGAAGAAGATAACAACAACGATCTGGCTATTACTGCCAACAGTGATGA
TGCGCCAGTGGTGCGCTTCGTTCACAAAGTACTGCTGGATGCCATCAAGCGTGGTGCATCGGATATCCACTTCGAGCCCT
ACGAATCCACCTATCGCATTCGTTTTCGTATCGATGGCATGTTGGTCGAGAATACCCGTCCACCCTTCAATATGCGTAAC
CGGATCTCGGCACGCCTGAAGGTCATGTCGCGCATGGATATTTCCGAGCGCCGTTTACCGCAGGACGGCGCGATCAAACT
GCGCCTCTCCAAGTCACGCTCCATCGACTTTCGCGTCAATACACTTCCCACTGTGTACGGCGAGAAACTGGTGCTGCGTA
TCCTGGACCCCAGCTCGGCCAAGATGGGCATCGAGGCGCTAGGCTTCGAACCCGAACAGCGTGCCATGTTCGAGAAGACG
CTGGATCAGCCCCAGGGCATGATTCTGGTCACTGGCCCGACCGGTAGCGGCAAGACCGTAACGCTCTACACTGGACTGAA
TATCCTCAATACTCCGGAGCGCAATATCTCGACGGCGGAAGACCCGGTCGAGCTCAAGGTAGAAGGCGTCAACCAGGTCA
ACGTGCTGCCCAAGATCGGCCTGGACTTCGCCTCGGCCCTGCGCGCCTTCCTGCGCCAGGATCCCGATGTAGTGATGGTG
GGGGAAATTCGTGACAAGGAAACCGCCGAGATCGCCGTCAAGGCGGCGCAGACTGGTCACCTGGTACTTTCTACCTTGCA
CACAAATTCTGCCCCCGAGACACTGACTCGCCTGAGCAACATGGGAATTGCCCCCTTCAATATCGCGAGTTCCGTCTCGT
TGATCATTGCTCAGCGCCTTGCTCGCCGCCTGTGTACCCACTGCCGACAGCCCACGGAGATTCCGCCCGAGGCGATGGCC
TCGATGGGCTTCAGTGAAGACAATGGCTTCAGCGCAGCAGAACTAGAGGCGGCGACCATATTCCGCCCCGTCGGTTGCAG
TCAGTGTACGCTGGGTTACAAGGGGCGAGTCGGCATCTATGAAGTCATGCCGATCTCGGATGAGATGAGCCGCTTGATCA
TGCGTGAGGGCACCGCCATCGAGCTGGCAGACCAGGCGCGCAAGGAAGGGCTTTCAGACCTGAGGCGTAGCGGCTTACGC
AAGGTGATGTCGGGCATGACCTCGCTGGAAGAAGTCAATCGCATCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.373

98.443

0.516
  pilB Acinetobacter baylyi ADP1

52.098

98.962

0.516
  pilB Legionella pneumophila strain ERS1305867

51.304

99.481

0.51
  pilB Vibrio parahaemolyticus RIMD 2210633

48.148

98.097

0.472
  pilB Vibrio cholerae strain A1552

46.564

100

0.469
  pilB Vibrio campbellii strain DS40M4

46.946

99.135

0.465
  pilF Neisseria gonorrhoeae MS11

47.662

96.194

0.458
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.715

99.654

0.386
  pilB/pilB1 Synechocystis sp. PCC 6803

42.015

91.003

0.382
  pilF Thermus thermophilus HB27

38.584

97.751

0.377