Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H2O77_RS03710 Genome accession   NZ_CP059843
Coordinates   872329..873189 (-) Length   286 a.a.
NCBI ID   WP_240548701.1    Uniprot ID   -
Organism   Cobetia sp. 4B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 867329..878189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2O77_RS03690 (H2O77_03685) argJ 869028..870251 (+) 1224 WP_215823928.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  H2O77_RS03695 (H2O77_03690) - 870359..871312 (+) 954 WP_043332483.1 Nudix family hydrolase -
  H2O77_RS03700 (H2O77_03695) yacG 871444..871689 (-) 246 WP_153635187.1 DNA gyrase inhibitor YacG -
  H2O77_RS03705 (H2O77_03700) coaE 871682..872332 (-) 651 WP_215823929.1 dephospho-CoA kinase -
  H2O77_RS03710 (H2O77_03705) pilD 872329..873189 (-) 861 WP_240548701.1 A24 family peptidase Machinery gene
  H2O77_RS03715 (H2O77_03710) pilC 873281..874519 (-) 1239 WP_200019032.1 type II secretion system F family protein Machinery gene
  H2O77_RS03720 (H2O77_03715) pilB 874695..876431 (-) 1737 WP_215823930.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H2O77_RS03725 (H2O77_03720) pilA2 877005..877445 (+) 441 WP_215823931.1 pilin Machinery gene

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31140.37 Da        Isoelectric Point: 8.2191

>NTDB_id=472186 H2O77_RS03710 WP_240548701.1 872329..873189(-) (pilD) [Cobetia sp. 4B]
MESLAQLSPTLQACLAGIFGLLVGSFLNVVITRLPVMMMRQWRAEAREALELEAEDTPRFNLLVPASRCPGCDTPIKWHD
NLPILGWLKRRGRCASCNTRISAQYPLVELAGGALGASIALIYGLEAQSLWIFGACLTLLVLSVIDFRTQLLPDAITLPL
LWAGLAYQLLFQPLMLPSAVIGAMAGYLVLWSFYWLFKLVTGKEGMGYGDFKLLAALGAWLGWQWLPLLLILSAGLGAIL
GILLQLLVPRLRGAPMPFGPYLAVAGWIALLFGTPLMSLYLSVIGA

Nucleotide


Download         Length: 861 bp        

>NTDB_id=472186 H2O77_RS03710 WP_240548701.1 872329..873189(-) (pilD) [Cobetia sp. 4B]
GTGGAATCACTCGCCCAACTCTCCCCCACACTGCAAGCCTGCCTGGCCGGTATATTCGGTTTGCTGGTCGGCAGCTTTCT
AAATGTCGTCATCACCCGCCTGCCAGTGATGATGATGCGCCAATGGCGCGCCGAGGCCCGCGAGGCATTGGAGCTGGAAG
CCGAAGACACACCGCGCTTCAATCTACTGGTGCCAGCATCACGCTGCCCGGGCTGTGACACGCCGATCAAGTGGCACGAT
AACCTCCCTATTCTCGGCTGGCTGAAGCGGCGTGGTCGCTGCGCCAGCTGCAATACGCGCATCAGCGCGCAGTACCCGCT
GGTGGAACTGGCCGGTGGCGCGCTTGGCGCTTCCATCGCGTTGATCTACGGCCTGGAGGCACAGAGCCTGTGGATCTTCG
GCGCCTGCCTGACTCTGCTGGTGCTATCGGTCATCGACTTCCGCACCCAGCTGCTGCCAGATGCCATCACATTGCCACTG
CTGTGGGCAGGCCTCGCCTATCAGTTGCTGTTTCAGCCTCTGATGCTACCCAGTGCCGTCATCGGCGCGATGGCGGGCTA
TCTGGTGCTGTGGAGCTTCTACTGGCTATTCAAGCTGGTCACCGGCAAGGAAGGCATGGGCTACGGTGACTTCAAGCTGC
TCGCCGCTCTCGGCGCCTGGCTCGGCTGGCAGTGGTTGCCGCTGTTGCTGATTCTGTCCGCCGGTCTCGGGGCCATTCTG
GGCATCCTGCTGCAACTGCTGGTACCGCGTCTGCGCGGCGCACCGATGCCCTTCGGGCCTTACCTGGCGGTTGCCGGCTG
GATCGCTTTGCTGTTTGGCACGCCCTTGATGAGCCTCTACCTGAGCGTGATCGGCGCATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-121)

Type IV leader peptidase family (Peptidase_A24)

(136-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.287

100

0.538
  pilD Vibrio campbellii strain DS40M4

47.518

98.601

0.469
  pilD Neisseria gonorrhoeae MS11

48.175

95.804

0.462
  pilD Acinetobacter baumannii D1279779

48.12

93.007

0.448
  pilD Acinetobacter nosocomialis M2

47.744

93.007

0.444