NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	MS8320_RS17745	Genome accession	NZ_CP059339
Coordinates	3599798..3600922 (-)	Length	374 a.a.
NCBI ID	WP_000228508.1	Uniprot ID	A0A0D8VMA7
Organism	Escherichia coli strain MS8320
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 3594798..3605922

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MS8320_RS17715 (MS8320_3453)	yrdA	3596598..3597152 (+)	555	WP_001286216.1	gamma carbonic anhydrase family protein	-
MS8320_RS17720 (MS8320_3454)	yrdB	3597128..3597385 (-)	258	WP_001070567.1	DUF1488 domain-containing protein	-
MS8320_RS17725 (MS8320_3455)	aroE	3597382..3598200 (-)	819	WP_000451230.1	shikimate dehydrogenase	-
MS8320_RS17730 (MS8320_3456)	tsaC	3598205..3598777 (-)	573	WP_001297709.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
MS8320_RS17735 (MS8320_3457)	yrdD	3598782..3599324 (-)	543	WP_001312137.1	type I DNA topoisomerase	-
MS8320_RS17740 (MS8320_3458)	smg	3599353..3599826 (-)	474	WP_000460672.1	DUF494 family protein Smg	-
MS8320_RS17745 (MS8320_3459)	dprA	3599798..3600922 (-)	1125	WP_000228508.1	DNA-protecting protein DprA	Machinery gene
MS8320_RS17750 (MS8320_3460)	def	3601052..3601561 (+)	510	WP_000114984.1	peptide deformylase	-
MS8320_RS17755 (MS8320_3461)	fmt	3601576..3602523 (+)	948	WP_000004421.1	methionyl-tRNA formyltransferase	-
MS8320_RS17760 (MS8320_3462)	rsmB	3602575..3603864 (+)	1290	WP_000744766.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
MS8320_RS17765 (MS8320_3463)	trkA	3603886..3605262 (+)	1377	WP_001539892.1	Trk system potassium transporter TrkA	-
MS8320_RS17770 (MS8320_3464)	mscL	3605392..3605805 (+)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40892.82 Da Isoelectric Point: 6.0395

                                >NTDB_id=468819 MS8320_RS17745 WP_000228508.1 3599798..3600922(-) (dprA) [Escherichia coli strain MS8320]

MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=468819 MS8320_RS17745 WP_000228508.1 3599798..3600922(-) (dprA) [Escherichia coli strain MS8320]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATTAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (DprA_WH)

(312-357)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0D8VMA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	43.917	90.107	0.396
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0468819