Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HZY77_RS14035 Genome accession   NZ_CP059257
Coordinates   2851874..2852926 (-) Length   350 a.a.
NCBI ID   WP_296816026.1    Uniprot ID   -
Organism   MAG: Thiobacillus sp. isolate SSC5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2846874..2857926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZY77_RS14005 - 2846997..2847371 (-) 375 WP_296816009.1 SlyX family protein -
  HZY77_RS14010 (HZY77_13860) - 2847368..2847904 (-) 537 WP_296816012.1 TlpA disulfide reductase family protein -
  HZY77_RS14015 (HZY77_13865) - 2847903..2848316 (+) 414 WP_296816015.1 CopD family protein -
  HZY77_RS14020 (HZY77_13870) - 2848496..2849569 (+) 1074 WP_296816018.1 THUMP domain-containing protein -
  HZY77_RS14025 (HZY77_13875) - 2849628..2850596 (+) 969 WP_296816021.1 restriction endonuclease -
  HZY77_RS14030 (HZY77_13880) pilU 2850743..2851870 (-) 1128 WP_296816023.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HZY77_RS14035 (HZY77_13885) pilT 2851874..2852926 (-) 1053 WP_296816026.1 type IV pilus twitching motility protein PilT Machinery gene
  HZY77_RS14040 (HZY77_13890) - 2852976..2853718 (+) 743 Protein_2776 YggS family pyridoxal phosphate-dependent enzyme -
  HZY77_RS14045 (HZY77_13895) proC 2853932..2854747 (+) 816 WP_296816030.1 pyrroline-5-carboxylate reductase -
  HZY77_RS14050 (HZY77_13900) - 2854744..2855319 (+) 576 WP_296816033.1 YggT family protein -
  HZY77_RS14055 (HZY77_13905) - 2855466..2855777 (+) 312 WP_296655592.1 DUF167 domain-containing protein -
  HZY77_RS14060 (HZY77_13910) - 2855774..2857678 (+) 1905 WP_296816036.1 dynamin family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38759.13 Da        Isoelectric Point: 6.4178

>NTDB_id=468183 HZY77_RS14035 WP_296816026.1 2851874..2852926(-) (pilT) [MAG: Thiobacillus sp. isolate SSC5]
MDISELLAFAVKNKASDLHLSASLPPMIRVNGDIRRINLPPMDHKDVHRMVYDIMNDSQRKVYEETLEIDFSFEIPSLAR
FRVNAFNQQYGAGAVFRTIPSKVLTLEELNAPPIFKEISDQPRGIVLVTGPTGSGKSTTLAAMMDYVNENQYAHILTVED
PIEFVHQSKKCLINQREVGPHTLSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGNGRVAAHEIMIGTPAIRNLIRENKVAQMYSSIQTGGNLGMQTLDQC
LQDLVRRNVVAPGVARAAAQNKDXPSWAED

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=468183 HZY77_RS14035 WP_296816026.1 2851874..2852926(-) (pilT) [MAG: Thiobacillus sp. isolate SSC5]
GTGGATATTTCCGAACTGCTGGCTTTTGCCGTCAAGAACAAGGCGTCCGACTTACACCTCTCTGCAAGCCTGCCGCCGAT
GATCCGCGTCAACGGCGACATCCGCCGCATCAACCTGCCGCCGATGGACCACAAGGACGTGCACCGCATGGTGTACGACA
TCATGAACGACAGCCAGCGCAAGGTGTACGAGGAAACGCTGGAAATCGACTTCTCGTTCGAGATCCCTTCCCTCGCGCGC
TTCCGCGTCAACGCCTTCAACCAGCAATACGGTGCCGGCGCGGTATTCCGGACGATTCCGTCGAAAGTGCTGACGCTGGA
AGAACTCAACGCACCACCCATCTTCAAGGAAATCTCCGACCAGCCGCGCGGCATCGTGTTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCCGCGATGATGGACTACGTCAACGAGAACCAGTACGCGCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACCAGAGCAAGAAATGCCTGATCAACCAGCGCGAGGTCGGTCCACATACGCTATCGTTCAACAA
CGCCCTGCGCTCGGCGCTGCGGGAAGACCCGGACGTCATCCTGGTCGGCGAATTGCGCGATCTGGAAACCATCCGCCTCG
CGCTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCATACGTCGTCCGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCTGCGGCAGAAAAGGAAATGGTGCGTTCGATGCTGTCCGAATCGCTGCGCGCCGTGATTTCGCAGAC
GCTGCTGAAGACCAAGGACGGCAACGGCCGCGTCGCCGCGCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGGTCGCGCAGATGTATTCGTCGATCCAGACCGGCGGCAACCTCGGCATGCAGACGCTCGACCAGTGC
CTGCAGGACCTGGTCCGGCGCAATGTGGTCGCCCCGGGCGTGGCGCGTGCCGCCGCGCAGAACAAAGACGNNCCTTCATG
GGCTGAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80

97.143

0.777
  pilT Acinetobacter baumannii D1279779

80

97.143

0.777
  pilT Acinetobacter baumannii strain A118

80

97.143

0.777
  pilT Pseudomonas aeruginosa PAK

77.059

97.143

0.749
  pilT Pseudomonas stutzeri DSM 10701

76.54

97.429

0.746
  pilT Acinetobacter baylyi ADP1

75.953

97.429

0.74
  pilT Legionella pneumophila strain Lp02

73.684

97.714

0.72
  pilT Legionella pneumophila strain ERS1305867

73.684

97.714

0.72
  pilT Neisseria meningitidis 8013

72.334

99.143

0.717
  pilT Neisseria gonorrhoeae MS11

71.758

99.143

0.711
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.697

94.286

0.657
  pilT Vibrio cholerae strain A1552

69.697

94.286

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.424

94.286

0.494
  pilU Pseudomonas stutzeri DSM 10701

42.901

92.571

0.397
  pilU Acinetobacter baylyi ADP1

42.462

92.857

0.394
  pilU Vibrio cholerae strain A1552

40.615

92.857

0.377