Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HZY77_RS14030 Genome accession   NZ_CP059257
Coordinates   2850743..2851870 (-) Length   375 a.a.
NCBI ID   WP_296816023.1    Uniprot ID   A0A7L5YHI6
Organism   MAG: Thiobacillus sp. isolate SSC5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2845743..2856870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZY77_RS14000 (HZY77_13850) - 2846554..2847000 (+) 447 WP_296816007.1 YhcH/YjgK/YiaL family protein -
  HZY77_RS14005 - 2846997..2847371 (-) 375 WP_296816009.1 SlyX family protein -
  HZY77_RS14010 (HZY77_13860) - 2847368..2847904 (-) 537 WP_296816012.1 TlpA disulfide reductase family protein -
  HZY77_RS14015 (HZY77_13865) - 2847903..2848316 (+) 414 WP_296816015.1 CopD family protein -
  HZY77_RS14020 (HZY77_13870) - 2848496..2849569 (+) 1074 WP_296816018.1 THUMP domain-containing protein -
  HZY77_RS14025 (HZY77_13875) - 2849628..2850596 (+) 969 WP_296816021.1 restriction endonuclease -
  HZY77_RS14030 (HZY77_13880) pilU 2850743..2851870 (-) 1128 WP_296816023.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HZY77_RS14035 (HZY77_13885) pilT 2851874..2852926 (-) 1053 WP_296816026.1 type IV pilus twitching motility protein PilT Machinery gene
  HZY77_RS14040 (HZY77_13890) - 2852976..2853718 (+) 743 Protein_2776 YggS family pyridoxal phosphate-dependent enzyme -
  HZY77_RS14045 (HZY77_13895) proC 2853932..2854747 (+) 816 WP_296816030.1 pyrroline-5-carboxylate reductase -
  HZY77_RS14050 (HZY77_13900) - 2854744..2855319 (+) 576 WP_296816033.1 YggT family protein -
  HZY77_RS14055 (HZY77_13905) - 2855466..2855777 (+) 312 WP_296655592.1 DUF167 domain-containing protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41974.04 Da        Isoelectric Point: 6.4360

>NTDB_id=468182 HZY77_RS14030 WP_296816023.1 2850743..2851870(-) (pilU) [MAG: Thiobacillus sp. isolate SSC5]
MNAEKTIHDLLRLMLAKNASDLFITAGFPXAIKVDGKITPVSNQSLTPAHTSELARSIMNERQWKDFEATNESNFAISPP
EIGRFRVNVFVQQGRVGVVMRTINTKIPKMEELNLPPILRDVAMIKRGLVIFVGGTGTGKSTSLAAMVGYRNENAADHII
TVEDPIEYVHEHKKSIITQREVGIDTDNWFSALKNTLRQAPDVILIGEIRDRDTMEYAIAFAETGHLCLSTLHANSANQA
LDRIINFFPEEKRDQLLMDLSLNLKSFISQRLIPRKGSNGRVAAVEILLNSPLIADLIFKGHVHEIKEIMAKSRELGMQT
FDQALFDLYEAGMISYEDALRNADSLNDLRLQIKLHGQEAKGRDMMAGLDHLDIV

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=468182 HZY77_RS14030 WP_296816023.1 2850743..2851870(-) (pilU) [MAG: Thiobacillus sp. isolate SSC5]
ATGAATGCCGAGAAAACCATCCACGACCTGCTGCGTCTGATGCTGGCGAAGAATGCCTCCGACCTGTTCATCACGGCCGG
CTTTCCCNCAGCCATCAAGGTCGACGGCAAGATCACCCCGGTGTCGAACCAGAGCCTGACCCCCGCGCATACCAGCGAAC
TGGCGCGCTCGATCATGAACGAGCGGCAATGGAAGGACTTCGAGGCCACCAACGAATCCAACTTCGCGATCAGCCCGCCC
GAGATCGGCCGCTTCCGCGTCAACGTCTTCGTGCAGCAGGGCCGCGTCGGCGTGGTGATGCGGACCATCAACACCAAGAT
CCCGAAGATGGAGGAGCTGAACCTGCCGCCGATCCTGCGCGACGTCGCCATGATCAAGCGCGGCCTGGTGATCTTCGTCG
GCGGTACCGGTACCGGTAAATCGACTTCGCTGGCGGCGATGGTGGGCTACCGCAACGAAAACGCCGCCGACCACATCATT
ACGGTCGAGGACCCGATCGAATACGTGCACGAGCACAAGAAATCCATCATCACCCAGCGCGAGGTCGGCATCGACACCGA
CAACTGGTTCTCCGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTCATCCTGATCGGCGAAATCCGCGACCGCGACA
CCATGGAATACGCGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGTCGACCCTGCACGCCAACAGCGCCAACCAGGCG
CTCGACCGCATCATCAACTTTTTCCCCGAGGAAAAGCGCGACCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGTCCTT
CATCTCGCAGCGCCTGATCCCGAGGAAAGGCAGCAACGGCCGCGTCGCCGCGGTCGAGATCCTGCTCAACTCGCCGCTGA
TCGCCGACCTGATCTTCAAGGGCCACGTCCACGAGATCAAGGAAATCATGGCGAAGTCGCGCGAGCTCGGCATGCAGACC
TTCGACCAGGCGCTGTTCGACCTCTACGAGGCCGGGATGATTTCCTACGAGGATGCGCTACGCAACGCCGACAGCCTCAA
CGACCTACGCCTGCAGATCAAGCTGCACGGCCAGGAAGCCAAGGGGCGCGACATGATGGCGGGGCTGGATCACCTCGATA
TTGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.894

95.467

0.581
  pilU Acinetobacter baylyi ADP1

59.104

95.2

0.563
  pilU Vibrio cholerae strain A1552

56.012

90.933

0.509
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.023

91.467

0.403
  pilT Acinetobacter baylyi ADP1

43.953

90.4

0.397
  pilT Pseudomonas aeruginosa PAK

43.62

89.867

0.392
  pilT Acinetobacter baumannii D1279779

43.844

88.8

0.389
  pilT Acinetobacter baumannii strain A118

43.844

88.8

0.389
  pilT Acinetobacter nosocomialis M2

43.844

88.8

0.389
  pilT Pseudomonas stutzeri DSM 10701

41.84

89.867

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.867

88.533

0.371
  pilT Vibrio cholerae strain A1552

41.867

88.533

0.371
  pilT Legionella pneumophila strain Lp02

41.441

88.8

0.368
  pilT Legionella pneumophila strain ERS1305867

41.441

88.8

0.368