Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6J83_RS20195 Genome accession   NZ_CP069549
Coordinates   4234269..4235249 (+) Length   326 a.a.
NCBI ID   WP_001305314.1    Uniprot ID   A0A0H2Z2A2
Organism   Escherichia coli strain FDAARGOS_1253     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4229269..4240249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J83_RS20165 (I6J83_20165) yggM 4229870..4230877 (+) 1008 WP_000745185.1 DUF1202 family protein -
  I6J83_RS20170 (I6J83_20170) hemW 4230946..4232082 (-) 1137 WP_000239975.1 radical SAM family heme chaperone HemW -
  I6J83_RS20175 (I6J83_20175) rdgB 4232075..4232668 (-) 594 WP_001174737.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  I6J83_RS20180 (I6J83_20180) yggU 4232676..4232966 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  I6J83_RS20185 (I6J83_20185) yggT 4232963..4233529 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  I6J83_RS20190 (I6J83_20190) yggS 4233547..4234251 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  I6J83_RS20195 (I6J83_20195) pilT 4234269..4235249 (+) 981 WP_001305314.1 type IV pilus twitching motility protein PilT Machinery gene
  I6J83_RS20200 (I6J83_20200) ruvX 4235423..4235839 (-) 417 WP_000017104.1 Holliday junction resolvase RuvX -
  I6J83_RS20205 (I6J83_20205) yqgE 4235839..4236402 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  I6J83_RS20210 (I6J83_20210) gshB 4236511..4237461 (-) 951 WP_000593257.1 glutathione synthase -
  I6J83_RS20215 (I6J83_20215) rsmE 4237474..4238205 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  I6J83_RS20220 (I6J83_20220) endA 4238285..4238992 (-) 708 WP_001327408.1 deoxyribonuclease I -
  I6J83_RS20225 (I6J83_20225) yggI 4239087..4239584 (-) 498 WP_001338010.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36043.27 Da        Isoelectric Point: 5.9975

>NTDB_id=468074 I6J83_RS20195 WP_001305314.1 4234269..4235249(+) (pilT) [Escherichia coli strain FDAARGOS_1253]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=468074 I6J83_RS20195 WP_001305314.1 4234269..4235249(+) (pilT) [Escherichia coli strain FDAARGOS_1253]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z2A2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362