Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H0S56_RS10905 Genome accession   NZ_CP059081
Coordinates   2272954..2274075 (+) Length   373 a.a.
NCBI ID   WP_004646138.1    Uniprot ID   N8NNG2
Organism   Acinetobacter lwoffii strain 12CE1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2267954..2279075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0S56_RS10890 (H0S56_10885) - 2269935..2271041 (+) 1107 WP_406935375.1 acyltransferase family protein -
  H0S56_RS10895 (H0S56_10890) - 2271073..2271762 (-) 690 WP_195726084.1 YggS family pyridoxal phosphate-dependent enzyme -
  H0S56_RS10900 (H0S56_10895) pilT 2271886..2272923 (+) 1038 WP_004279279.1 type IV pilus twitching motility protein PilT Machinery gene
  H0S56_RS10905 (H0S56_10900) pilU 2272954..2274075 (+) 1122 WP_004646138.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H0S56_RS10910 (H0S56_10905) fur 2274144..2274581 (-) 438 WP_004279276.1 ferric iron uptake transcriptional regulator -
  H0S56_RS10915 (H0S56_10910) - 2274694..2275092 (+) 399 WP_004279275.1 outer membrane protein assembly factor BamE -
  H0S56_RS10920 (H0S56_10915) - 2275193..2275525 (-) 333 WP_005247100.1 RnfH family protein -
  H0S56_RS10925 (H0S56_10920) - 2275525..2276604 (-) 1080 WP_114541547.1 hypothetical protein -
  H0S56_RS10930 (H0S56_10925) - 2276748..2277194 (-) 447 WP_004279272.1 bacteriohemerythrin -
  H0S56_RS10935 (H0S56_10930) - 2277469..2278317 (-) 849 WP_004730575.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41661.91 Da        Isoelectric Point: 6.8891

>NTDB_id=467133 H0S56_RS10905 WP_004646138.1 2272954..2274075(+) (pilU) [Acinetobacter lwoffii strain 12CE1]
MDFNGLLDYMVEKKASDLFITADVEPSIKINGQILPIGSVKLPGAAVGQLLNSIMTEKQRKEFADSRECNFAISTPDKSA
RFRVSAFQQRDQPGMVLRRIETVIPTMDELKLPPILKELAMTKRGIIIFVGATGTGKSTSLASLVGYRNENSKGHIITIE
DPIEFIHQHKGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNMKAIVAQQLIPTIDGNGRRAAIEILINSPLIADLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGSHLLRAHSNITFDGQS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=467133 H0S56_RS10905 WP_004646138.1 2272954..2274075(+) (pilU) [Acinetobacter lwoffii strain 12CE1]
ATGGATTTTAACGGCTTACTCGATTATATGGTGGAGAAAAAAGCATCGGATCTGTTCATTACTGCCGATGTCGAACCATC
CATCAAGATTAATGGTCAGATTTTACCGATTGGCTCAGTAAAATTACCGGGTGCGGCAGTAGGACAACTGCTCAATTCCA
TCATGACCGAAAAGCAGCGTAAAGAATTTGCTGACAGTCGTGAATGTAACTTTGCCATCAGCACACCGGACAAAAGTGCC
CGCTTCCGTGTCAGTGCCTTTCAGCAACGCGATCAACCGGGGATGGTTTTGCGTCGGATTGAAACTGTTATTCCAACTAT
GGATGAACTCAAGTTACCGCCAATTTTAAAAGAACTGGCGATGACCAAACGTGGCATCATTATTTTTGTCGGGGCAACGG
GTACCGGTAAATCAACCTCGCTGGCGTCTTTGGTCGGTTATCGCAATGAAAATTCCAAAGGTCATATCATTACCATTGAA
GATCCGATCGAATTTATTCACCAGCATAAGGGCTGTATTATCACCCAGCGTGAAGTCGGCATCGATACTGATTCATTTGA
AATTGCCCTGAAAAATACCCTGCGTCAGGCACCAGACGTGATTCTGATCGGTGAGATCCGTTCACGTGAAACCATGGATT
ATGCGATTGCCTTTGCCGAAACCGGTCATCTGGTCTTTGCTACCCTGCACGCCAACAACGCCAACCAAGCGATTGACCGG
ATTATCCATTTCTTCGAAGCAGACCGACATAATCAGCTGTTCATGGACTTGTCCCTGAACATGAAAGCCATTGTGGCACA
GCAACTGATTCCAACCATCGATGGTAATGGTCGCCGTGCAGCGATTGAGATTCTGATTAACTCACCACTGATTGCCGACT
TGATTCGTAAAGGTGATGTGCATGAGATCAAGGATCTGATGAAGCGTTCCCGTGAACTGGGCATGCAGACCTTCGATCAG
GCGCTGTATGATTTGTATAAAGCCAAACAGATCAGCTATAAAGATGCGCTGAAACATGCGGACTCACCCAACGATCTGCG
TCTGCAAATCAAACTGTCAGAAGAAGGTGGCAGCCACTTATTACGTGCACATTCCAATATTACCTTTGATGGTCAGTCCT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N8NNG2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

72.222

100

0.732
  pilU Pseudomonas stutzeri DSM 10701

68.733

99.464

0.684
  pilU Vibrio cholerae strain A1552

57.265

94.102

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

92.761

0.394
  pilT Acinetobacter baumannii strain A118

41.691

91.957

0.383
  pilT Acinetobacter nosocomialis M2

41.691

91.957

0.383
  pilT Acinetobacter baumannii D1279779

41.691

91.957

0.383
  pilT Legionella pneumophila strain ERS1305867

42.262

90.08

0.381
  pilT Legionella pneumophila strain Lp02

42.262

90.08

0.381
  pilT Pseudomonas aeruginosa PAK

40.462

92.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.601

94.102

0.373
  pilT Vibrio cholerae strain A1552

39.601

94.102

0.373
  pilT Acinetobacter baylyi ADP1

40.233

91.957

0.37
  pilT Neisseria gonorrhoeae MS11

39.595

92.761

0.367
  pilT Neisseria meningitidis 8013

39.595

92.761

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.017

92.761

0.362