Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H0S56_RS10900 Genome accession   NZ_CP059081
Coordinates   2271886..2272923 (+) Length   345 a.a.
NCBI ID   WP_004279279.1    Uniprot ID   N9QEA8
Organism   Acinetobacter lwoffii strain 12CE1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2266886..2277923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0S56_RS10890 (H0S56_10885) - 2269935..2271041 (+) 1107 WP_406935375.1 acyltransferase family protein -
  H0S56_RS10895 (H0S56_10890) - 2271073..2271762 (-) 690 WP_195726084.1 YggS family pyridoxal phosphate-dependent enzyme -
  H0S56_RS10900 (H0S56_10895) pilT 2271886..2272923 (+) 1038 WP_004279279.1 type IV pilus twitching motility protein PilT Machinery gene
  H0S56_RS10905 (H0S56_10900) pilU 2272954..2274075 (+) 1122 WP_004646138.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H0S56_RS10910 (H0S56_10905) fur 2274144..2274581 (-) 438 WP_004279276.1 ferric iron uptake transcriptional regulator -
  H0S56_RS10915 (H0S56_10910) - 2274694..2275092 (+) 399 WP_004279275.1 outer membrane protein assembly factor BamE -
  H0S56_RS10920 (H0S56_10915) - 2275193..2275525 (-) 333 WP_005247100.1 RnfH family protein -
  H0S56_RS10925 (H0S56_10920) - 2275525..2276604 (-) 1080 WP_114541547.1 hypothetical protein -
  H0S56_RS10930 (H0S56_10925) - 2276748..2277194 (-) 447 WP_004279272.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38189.82 Da        Isoelectric Point: 6.5056

>NTDB_id=467132 H0S56_RS10900 WP_004279279.1 2271886..2272923(+) (pilT) [Acinetobacter lwoffii strain 12CE1]
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDFEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEDLGMGQIFKDICEYPRGLVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVVSQALLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQTL
KTLVSKGLISPQVARTAAKQPEAFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=467132 H0S56_RS10900 WP_004279279.1 2271886..2272923(+) (pilT) [Acinetobacter lwoffii strain 12CE1]
ATGGATATCACAGAATTGTTGGCATTTTCTGCTAAAAATGGTGCGTCGGATTTACACTTGTCTGCGGGCTTACCGCCAAT
GATTCGTGTCGATGGGGAAGTTCGTCGCATCAACCTGCCAGCATTAGAACACAAAGAAGTGCATAAACTCGTTTATGACA
TCATGAACGATAAACAGCGCCGTGATTTTGAAGAAAATCTGGAAACTGACTTTTCTTTTGAAGTGCCTGGCGTGGCACGT
TTCCGTGTCAACGCATTTAACCAGAACCGCGGTGCCGGTGCGGTATTCCGTACCATTCCCTCTAAAGTACTGACCCTTGA
AGATCTGGGCATGGGACAGATTTTTAAAGATATCTGTGAATATCCACGTGGTCTGGTACTAGTCACAGGTCCAACCGGTT
CGGGTAAATCAACTACCTTGGCGGCGATGCTGGATTATATTAATGACAACCGCTATGACCATATTCTGACCGTCGAAGAT
CCGATCGAATTTGTGCATCAGTCCAAGAAATGTTTGATTAACCAGCGTGAAGTGCATCGTGATACGCATGGCTTTAATGA
AGCATTACGTTCTGCATTACGTGAAGATCCGGATATTATTCTGGTCGGTGAGATGCGTGACCTTGAAACCATTCGTCTGG
CTTTAACTGCGGCAGAAACCGGCCACTTAGTATTTGGTACCCTGCATACCACCTCAGCGGCCAAAACCATTGACCGTGTG
ATCGACGTATTCCCTGCTGAAGAAAAAGACATGGTACGTGCCATGTTGTCTGAATCCCTACAAGCCGTAGTTTCACAGGC
TCTGCTGAAGAAAAATGGCGGTGGGCGTGTGGCGGCGCATGAAATCATGATTGGTATTCCGGCGATTCGTAACCTAATTC
GTGAAAACAAAGTCGCACAAATGTACTCAGCAATTCAGACCGGTGCCAACTATGGCATGACCACACTGGACCAGACCCTG
AAAACACTGGTGTCCAAAGGTTTGATCAGCCCGCAAGTTGCACGTACTGCAGCCAAACAGCCAGAAGCATTTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9QEA8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

93.913

100

0.939
  pilT Acinetobacter baumannii D1279779

93.623

100

0.936
  pilT Acinetobacter baumannii strain A118

93.623

100

0.936
  pilT Acinetobacter baylyi ADP1

86.337

99.71

0.861
  pilT Pseudomonas aeruginosa PAK

84.884

99.71

0.846
  pilT Pseudomonas stutzeri DSM 10701

84.593

99.71

0.843
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Neisseria meningitidis 8013

68.406

100

0.684
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.88

96.232

0.672
  pilT Vibrio cholerae strain A1552

69.88

96.232

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.525
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.692

95.942

0.4
  pilU Acinetobacter baylyi ADP1

38.776

99.42

0.386