Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6J32_RS12090 Genome accession   NZ_CP069437
Coordinates   2517182..2518231 (-) Length   349 a.a.
NCBI ID   WP_128680027.1    Uniprot ID   -
Organism   Moraxella osloensis strain FDAARGOS_1202     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2512182..2523231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J32_RS12075 (I6J32_12095) ribF 2514194..2515207 (-) 1014 WP_204155708.1 riboflavin biosynthesis protein RibF -
  I6J32_RS12080 (I6J32_12100) - 2515333..2515869 (+) 537 WP_204155709.1 GNAT family N-acetyltransferase -
  I6J32_RS12085 (I6J32_12105) pilU 2515941..2517092 (-) 1152 WP_204155710.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6J32_RS12090 (I6J32_12110) pilT 2517182..2518231 (-) 1050 WP_128680027.1 type IV pilus twitching motility protein PilT Machinery gene
  I6J32_RS12095 (I6J32_12115) - 2518499..2519224 (+) 726 WP_204155711.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6J32_RS12100 (I6J32_12120) - 2519650..2520174 (+) 525 WP_204155712.1 C40 family peptidase -
  I6J32_RS12105 (I6J32_12125) - 2520511..2521860 (+) 1350 WP_204155713.1 sensor domain-containing diguanylate cyclase -
  I6J32_RS12110 (I6J32_12130) truD 2521897..2522985 (-) 1089 WP_204155714.1 tRNA pseudouridine(13) synthase TruD -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38932.62 Da        Isoelectric Point: 6.7822

>NTDB_id=466610 I6J32_RS12090 WP_128680027.1 2517182..2518231(-) (pilT) [Moraxella osloensis strain FDAARGOS_1202]
MAKELSIDDLLRFAFKNKASDLHISAGLPPMIRVDGEITKINMPPLDHQKVHSLIYDIMNDKQRADYEEFFETDFSFEIP
NVARFRVNAFNQNRGAGAVFRTIPSEVLTMEQLGMGPIFEKISNYKRGVVLVTGPTGSGKSTTLAAMINYINENRKEHIL
TIEDPIEFVHQSKKSLINQREVHRDTLSFDAALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKT
IDRVVDVFPAAEKDMIRAMLSESLQAVISQSLLKRQTGGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTSAGDGMTTL
DQSLRELVNKGVISKESARQYAKQPESFM

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=466610 I6J32_RS12090 WP_128680027.1 2517182..2518231(-) (pilT) [Moraxella osloensis strain FDAARGOS_1202]
ATGGCAAAGGAATTATCGATTGACGATTTGTTGCGTTTTGCATTCAAAAATAAAGCCTCTGACTTACATATTTCAGCAGG
CTTACCGCCGATGATTCGGGTAGATGGTGAAATCACCAAAATCAACATGCCACCGCTCGATCACCAAAAAGTGCATAGTT
TGATTTATGACATCATGAATGACAAACAACGTGCCGATTATGAAGAGTTTTTTGAAACTGACTTTTCGTTTGAAATTCCC
AATGTGGCGCGCTTTCGTGTCAATGCCTTTAACCAAAACCGTGGCGCAGGTGCGGTATTTCGTACCATTCCGTCTGAGGT
CTTGACGATGGAGCAACTTGGGATGGGACCGATTTTTGAGAAAATCTCTAACTACAAACGCGGCGTGGTACTGGTCACAG
GTCCAACCGGTTCGGGTAAGTCAACCACCCTTGCGGCGATGATTAACTATATCAACGAAAACCGTAAAGAGCATATCTTA
ACCATCGAAGACCCGATTGAATTTGTGCATCAATCAAAAAAATCCCTTATCAACCAACGGGAAGTCCACCGTGATACCTT
AAGCTTTGACGCCGCGCTGCGCTCTGCCCTACGTGAAGACCCCGATGTTATTCTTGTCGGTGAGATGCGTGACCTTGAGA
CCATCCGCTTGGCATTGACCGCTGCTGAAACAGGGCACTTGGTATTTGGGACATTACATACCTCATCTGCTGCCAAAACC
ATTGACCGTGTGGTCGATGTGTTCCCCGCCGCTGAAAAAGACATGATTCGTGCGATGTTATCTGAATCGCTGCAAGCGGT
TATTTCGCAATCGCTACTCAAACGCCAAACGGGTGGTCGTGTCGCCGCCCATGAAATCATGATTGGTACGCCTGCGATTC
GTAACTTGATTCGTGAAAACAAAGTCGCTCAAATGTACTCTGCCATCCAAACCAGTGCAGGCGATGGTATGACCACACTT
GATCAAAGTCTTCGCGAACTGGTCAATAAAGGCGTGATTAGCAAAGAATCGGCGCGCCAATATGCTAAACAACCGGAAAG
CTTTATGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(14-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.58

98.854

0.797
  pilT Acinetobacter baumannii D1279779

80.58

98.854

0.797
  pilT Acinetobacter baumannii strain A118

80.58

98.854

0.797
  pilT Acinetobacter baylyi ADP1

77.035

98.567

0.759
  pilT Pseudomonas aeruginosa PAK

77.035

98.567

0.759
  pilT Pseudomonas stutzeri DSM 10701

76.744

98.567

0.756
  pilT Legionella pneumophila strain Lp02

73.256

98.567

0.722
  pilT Legionella pneumophila strain ERS1305867

73.256

98.567

0.722
  pilT Neisseria meningitidis 8013

67.536

98.854

0.668
  pilT Neisseria gonorrhoeae MS11

67.246

98.854

0.665
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.976

95.129

0.656
  pilT Vibrio cholerae strain A1552

68.976

95.129

0.656
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.149

100

0.521
  pilU Acinetobacter baylyi ADP1

41.04

99.14

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.003

97.135

0.398
  pilU Vibrio cholerae strain A1552

40

95.989

0.384