Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6J32_RS12085 Genome accession   NZ_CP069437
Coordinates   2515941..2517092 (-) Length   383 a.a.
NCBI ID   WP_204155710.1    Uniprot ID   -
Organism   Moraxella osloensis strain FDAARGOS_1202     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2510941..2522092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J32_RS12070 (I6J32_12090) ileS 2511103..2513973 (-) 2871 WP_204155707.1 isoleucine--tRNA ligase -
  I6J32_RS12075 (I6J32_12095) ribF 2514194..2515207 (-) 1014 WP_204155708.1 riboflavin biosynthesis protein RibF -
  I6J32_RS12080 (I6J32_12100) - 2515333..2515869 (+) 537 WP_204155709.1 GNAT family N-acetyltransferase -
  I6J32_RS12085 (I6J32_12105) pilU 2515941..2517092 (-) 1152 WP_204155710.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6J32_RS12090 (I6J32_12110) pilT 2517182..2518231 (-) 1050 WP_128680027.1 type IV pilus twitching motility protein PilT Machinery gene
  I6J32_RS12095 (I6J32_12115) - 2518499..2519224 (+) 726 WP_204155711.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6J32_RS12100 (I6J32_12120) - 2519650..2520174 (+) 525 WP_204155712.1 C40 family peptidase -
  I6J32_RS12105 (I6J32_12125) - 2520511..2521860 (+) 1350 WP_204155713.1 sensor domain-containing diguanylate cyclase -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42957.27 Da        Isoelectric Point: 6.1918

>NTDB_id=466609 I6J32_RS12085 WP_204155710.1 2515941..2517092(-) (pilU) [Moraxella osloensis strain FDAARGOS_1202]
MDFDKLLQLMVQKNGSDLFITADAPPSMKVNGTIMPVTKSALTPEQSMTLVKSVMTPSQIKEFDETLECQFAISDKHQNA
RFRVSAFVQKDCAGMVLRKIETKIPTVEQLNLPPILNEIAMKKRGIVLLVGATGTGKSTTLAAMMGYRNENSRGHIITIE
DPIEYVHEHKGCIITQREVGIDTKSFEAGLKNTLRQAPDVILIGEIRTRETMDYAIQYAETGHLVFATLHANNANQAIDR
IIHFFDAEHHEQILMDLSLNLHAIVAQQLIATPDGKGRRAAIEILLNSQLIGDYIRKGEVHEIKPVMKRSRELGMQTFDQ
ALFDLYENGEITYRDAIKHADSPNDLRLLIKLESKSANKIDENELNFSLQSDDHSAANEKSMF

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=466609 I6J32_RS12085 WP_204155710.1 2515941..2517092(-) (pilU) [Moraxella osloensis strain FDAARGOS_1202]
ATGGATTTTGATAAATTACTGCAGCTAATGGTACAAAAAAACGGCTCTGACCTTTTTATCACCGCCGATGCGCCACCATC
AATGAAAGTCAATGGTACTATCATGCCAGTCACCAAGTCAGCGCTGACCCCAGAGCAATCGATGACGCTGGTCAAAAGCG
TGATGACACCTAGTCAAATCAAAGAATTTGACGAAACCCTCGAATGCCAGTTTGCCATTAGTGACAAACACCAAAACGCC
CGCTTTCGTGTTTCTGCCTTTGTCCAAAAAGACTGTGCGGGCATGGTGCTACGTAAAATTGAAACCAAAATTCCGACGGT
AGAGCAACTCAATCTACCGCCGATTTTGAATGAAATTGCCATGAAAAAACGCGGTATCGTGCTACTGGTCGGTGCGACAG
GTACAGGTAAATCTACCACGCTGGCGGCTATGATGGGTTATCGCAACGAGAATTCGCGCGGTCATATCATTACCATCGAA
GACCCGATTGAATACGTCCATGAGCACAAAGGCTGTATCATCACCCAACGCGAAGTTGGCATTGATACCAAATCATTTGA
AGCAGGGTTAAAAAACACCCTGCGGCAAGCGCCCGATGTGATTTTGATTGGCGAGATTCGTACCCGCGAAACCATGGATT
ATGCCATTCAATATGCCGAAACAGGACACTTGGTGTTTGCCACCCTACACGCCAACAATGCTAACCAAGCGATTGACCGT
ATCATTCACTTCTTTGACGCAGAACATCATGAGCAGATTTTGATGGATTTGTCATTGAACCTTCACGCCATTGTAGCGCA
GCAATTGATTGCCACCCCTGATGGCAAAGGTCGCCGCGCTGCGATTGAAATTTTGCTCAATTCGCAACTGATTGGTGATT
ACATCCGTAAAGGGGAAGTCCACGAAATTAAGCCCGTCATGAAACGCTCTCGTGAACTTGGCATGCAAACCTTTGACCAA
GCGTTGTTTGATTTGTATGAAAATGGCGAAATTACTTACCGCGATGCCATCAAACACGCTGACTCACCCAATGATTTACG
CCTACTCATTAAGCTGGAGAGTAAATCAGCCAATAAAATTGATGAAAATGAACTAAATTTTTCATTGCAATCTGATGATC
ATAGCGCAGCAAATGAGAAATCCATGTTCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.539

92.95

0.637
  pilU Acinetobacter baylyi ADP1

64.835

95.039

0.616
  pilU Vibrio cholerae strain A1552

54.986

91.645

0.504
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.735

91.645

0.392
  pilT Acinetobacter baumannii D1279779

42.274

89.556

0.379
  pilT Acinetobacter nosocomialis M2

42.274

89.556

0.379
  pilT Acinetobacter baumannii strain A118

42.274

89.556

0.379
  pilT Acinetobacter baylyi ADP1

41.691

89.556

0.373
  pilT Legionella pneumophila strain ERS1305867

42.262

87.728

0.371
  pilT Legionella pneumophila strain Lp02

42.262

87.728

0.371
  pilT Pseudomonas aeruginosa PAK

40.462

90.339

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.462

90.339

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.994

86.423

0.363
  pilT Vibrio cholerae strain A1552

41.994

86.423

0.363