Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HZU75_RS12205 Genome accession   NZ_CP058952
Coordinates   2554397..2555440 (+) Length   347 a.a.
NCBI ID   WP_180306307.1    Uniprot ID   -
Organism   Chitinibacter fontanus strain STM-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2549397..2560440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZU75_RS12170 (HZU75_12170) dsbD 2549532..2551085 (-) 1554 WP_308419414.1 protein-disulfide reductase DsbD -
  HZU75_RS12175 (HZU75_12175) cutA 2551082..2551432 (-) 351 WP_180306301.1 divalent-cation tolerance protein CutA -
  HZU75_RS12180 (HZU75_12180) - 2551431..2551874 (+) 444 WP_180306302.1 FxsA family protein -
  HZU75_RS12185 (HZU75_12185) - 2551876..2552163 (-) 288 WP_180306303.1 DUF167 family protein -
  HZU75_RS12190 (HZU75_12190) - 2552167..2552754 (-) 588 WP_180306304.1 YggT family protein -
  HZU75_RS12195 (HZU75_12195) proC 2552788..2553606 (-) 819 WP_180306305.1 pyrroline-5-carboxylate reductase -
  HZU75_RS12200 (HZU75_12200) - 2553603..2554310 (-) 708 WP_180306306.1 YggS family pyridoxal phosphate-dependent enzyme -
  HZU75_RS12205 (HZU75_12205) pilT 2554397..2555440 (+) 1044 WP_180306307.1 type IV pilus twitching motility protein PilT Machinery gene
  HZU75_RS12210 (HZU75_12210) pilU 2555464..2556600 (+) 1137 WP_180306308.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HZU75_RS12215 (HZU75_12215) - 2556628..2558814 (+) 2187 WP_180306309.1 FimV family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38251.03 Da        Isoelectric Point: 6.8801

>NTDB_id=466141 HZU75_RS12205 WP_180306307.1 2554397..2555440(+) (pilT) [Chitinibacter fontanus strain STM-7]
MEISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPAMDHKDVHDMVYDIMNDGQRKVYEDTLECDFSFEIPNLAR
FRVNAFVQNRGAGAVFRTIPSKVLTLEELGCPRVFQDIAMNPRGLVLVTGPTGSGKSTTLAAMINFINDNEYGHILTVED
PIEFVHQSKKCLVNQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRAMLSESLRAVISQTLLKTKDGTGRVAAHEIMIGIPAIRNLIRENKIAQMYSAIQTGSAHGMQTLDQC
LQNLVQRNIVSVAEARNKAAIPDNFKG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=466141 HZU75_RS12205 WP_180306307.1 2554397..2555440(+) (pilT) [Chitinibacter fontanus strain STM-7]
ATGGAAATCTCGGAACTCTTAGCGTTTTCGGTCAAGAATAAGGCATCCGATTTGCATCTGTCGGCAGGATTGCCACCGAT
GATTCGCGTTCATGGTGATGTACGTCGCATTAACTTGCCTGCAATGGATCATAAAGATGTCCATGACATGGTGTATGACA
TCATGAATGATGGTCAGCGCAAAGTGTACGAGGACACCCTAGAGTGCGATTTCTCTTTCGAAATCCCTAATCTGGCGCGG
TTCCGGGTGAATGCTTTTGTGCAAAACCGAGGGGCTGGTGCGGTTTTTCGGACGATTCCATCAAAAGTTCTGACCTTGGA
AGAACTAGGCTGTCCGCGTGTGTTTCAGGATATCGCCATGAACCCACGTGGTTTGGTTCTGGTTACCGGGCCAACAGGTT
CAGGTAAATCGACTACTTTGGCGGCGATGATTAACTTCATCAACGACAATGAATATGGTCATATTCTGACCGTGGAAGAC
CCGATCGAATTTGTACACCAGTCTAAAAAATGCTTGGTGAATCAGCGTGAAGTCGGGCCACATACCTTATCTTTCTCCAA
TGCGCTGCGCAGTGCATTGCGGGAAGACCCAGACGTTATTCTGGTGGGTGAGATGCGCGATCTGGAAACGATTCGCTTAG
CATTGACCGCTGCTGAAACGGGGCACTTGGTATTCGGTACACTGCACACAAGTTCCGCAGCCAAAACCATTGACCGGGTA
GTGGACGTATTCCCTGCCGCAGAAAAAGAAATGGTGCGTGCAATGTTGTCGGAGTCTTTACGCGCGGTAATTTCACAGAC
GCTACTGAAAACCAAAGATGGCACCGGTCGGGTGGCTGCGCATGAAATCATGATTGGCATCCCTGCAATTCGAAACCTGA
TTCGGGAAAATAAAATTGCCCAAATGTACTCGGCGATTCAGACAGGGTCAGCGCATGGGATGCAAACGCTGGACCAATGT
CTGCAAAATCTAGTGCAACGCAATATTGTGTCTGTAGCCGAGGCGCGCAATAAAGCCGCAATTCCGGATAACTTCAAAGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.841

99.424

0.784
  pilT Acinetobacter baumannii D1279779

78.841

99.424

0.784
  pilT Acinetobacter baumannii strain A118

78.841

99.424

0.784
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Pseudomonas stutzeri DSM 10701

77.101

99.424

0.767
  pilT Acinetobacter baylyi ADP1

76.522

99.424

0.761
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Neisseria meningitidis 8013

72.566

97.695

0.709
  pilT Neisseria gonorrhoeae MS11

72.271

97.695

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.047

97.406

0.663
  pilT Vibrio cholerae strain A1552

68.047

97.406

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

39.481

100

0.395