Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HZU75_RS12210 Genome accession   NZ_CP058952
Coordinates   2555464..2556600 (+) Length   378 a.a.
NCBI ID   WP_180306308.1    Uniprot ID   A0A7D5ZI10
Organism   Chitinibacter fontanus strain STM-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2550464..2561600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZU75_RS12175 (HZU75_12175) cutA 2551082..2551432 (-) 351 WP_180306301.1 divalent-cation tolerance protein CutA -
  HZU75_RS12180 (HZU75_12180) - 2551431..2551874 (+) 444 WP_180306302.1 FxsA family protein -
  HZU75_RS12185 (HZU75_12185) - 2551876..2552163 (-) 288 WP_180306303.1 DUF167 family protein -
  HZU75_RS12190 (HZU75_12190) - 2552167..2552754 (-) 588 WP_180306304.1 YggT family protein -
  HZU75_RS12195 (HZU75_12195) proC 2552788..2553606 (-) 819 WP_180306305.1 pyrroline-5-carboxylate reductase -
  HZU75_RS12200 (HZU75_12200) - 2553603..2554310 (-) 708 WP_180306306.1 YggS family pyridoxal phosphate-dependent enzyme -
  HZU75_RS12205 (HZU75_12205) pilT 2554397..2555440 (+) 1044 WP_180306307.1 type IV pilus twitching motility protein PilT Machinery gene
  HZU75_RS12210 (HZU75_12210) pilU 2555464..2556600 (+) 1137 WP_180306308.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HZU75_RS12215 (HZU75_12215) - 2556628..2558814 (+) 2187 WP_180306309.1 FimV family protein -
  HZU75_RS12220 (HZU75_12220) aceB 2558879..2560465 (-) 1587 WP_180306310.1 malate synthase A -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42535.77 Da        Isoelectric Point: 6.2392

>NTDB_id=466142 HZU75_RS12210 WP_180306308.1 2555464..2556600(+) (pilU) [Chitinibacter fontanus strain STM-7]
MEKEQAAKFMHDLLRHMRGKNASDLFITVDFPPAMKIDGRVTPVSNQLLTAQHTKELARAIMNDRQSEDFEANKECNFAI
SPGNMGRFRVNAFMQQGRVGMVLRTINSEIPKLAELNLPPVLEDIAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNENAYD
HIITIEDPIEYVHEHKNSIITQREVGVDTDSWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANSS
NQALDRIINFFPEERRQQLLMDLSLNLKAFVSQRLVPHASGKGRVAAVEVMLNSPLISELIFKGEVHEIKEIMKKSRELG
MQTFDQSLFDLFEQDRITYEDALRNADSVNDLRLQIKLNSADAKKRDVLAGLDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=466142 HZU75_RS12210 WP_180306308.1 2555464..2556600(+) (pilU) [Chitinibacter fontanus strain STM-7]
ATGGAAAAAGAACAAGCCGCGAAGTTTATGCATGATTTACTGCGCCATATGCGTGGAAAGAATGCTTCGGATTTATTTAT
TACGGTGGATTTTCCACCAGCAATGAAAATTGATGGGCGTGTGACGCCTGTGTCCAACCAATTATTGACGGCACAACATA
CCAAAGAGTTGGCGCGGGCGATTATGAATGATCGTCAATCGGAAGACTTTGAAGCTAATAAAGAATGTAACTTTGCCATT
AGTCCGGGCAATATGGGGCGCTTCCGGGTGAATGCATTTATGCAACAGGGCCGCGTGGGTATGGTGTTGCGAACCATCAA
CTCGGAAATCCCGAAGCTGGCCGAGTTAAATTTGCCCCCTGTACTGGAAGACATTGCTATGACCAAGCGTGGCTTGGTGA
TTTTCGTAGGCGGTACCGGTTCGGGTAAATCGACCTCGCTGGCAGCCATGATTGGCCATCGTAACGAAAATGCATACGAC
CATATCATTACGATTGAAGACCCAATTGAATATGTGCACGAACATAAAAATTCGATTATCACGCAACGTGAAGTCGGGGT
GGATACTGATTCATGGATGGCAGCGCTGAAGAATACTTTGCGCCAAGCGCCCGATGTGATTTTGATTGGTGAGATTCGCG
ACCGTGAAACGATGGATTATGCGATTGCCTTTGCTGAAACGGGCCATTTATGTATGGCTACCTTGCATGCAAACTCATCC
AATCAGGCGCTGGACCGGATTATCAACTTCTTCCCGGAAGAGCGTCGGCAGCAGTTGTTGATGGACTTATCCTTAAACTT
GAAAGCTTTTGTCTCACAGCGCTTGGTGCCTCATGCCTCGGGTAAGGGGCGAGTGGCAGCCGTTGAGGTGATGCTCAATT
CGCCGCTGATTTCTGAGCTGATTTTCAAAGGCGAAGTTCATGAAATCAAAGAAATCATGAAAAAATCGCGTGAATTAGGG
ATGCAAACTTTTGACCAATCCTTGTTTGATTTGTTCGAGCAAGATCGAATTACCTATGAAGATGCTTTACGCAATGCTGA
CTCGGTAAATGATTTGCGTCTGCAAATTAAGCTCAATAGTGCGGATGCAAAAAAACGGGATGTACTGGCTGGGCTCGATC
ATTTGGATATTGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D5ZI10

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.506

92.063

0.585
  pilU Acinetobacter baylyi ADP1

58.543

94.444

0.553
  pilU Vibrio cholerae strain A1552

53.803

93.915

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.866

88.624

0.415
  pilT Acinetobacter baylyi ADP1

43.425

86.508

0.376
  pilT Acinetobacter nosocomialis M2

42.991

84.921

0.365
  pilT Acinetobacter baumannii D1279779

42.991

84.921

0.365
  pilT Acinetobacter baumannii strain A118

42.991

84.921

0.365
  pilT Legionella pneumophila strain Lp02

41.692

87.566

0.365
  pilT Legionella pneumophila strain ERS1305867

41.692

87.566

0.365
  pilT Pseudomonas aeruginosa PAK

41.194

88.624

0.365
  pilT Pseudomonas stutzeri DSM 10701

40.896

88.624

0.362