Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HXW73_RS01615 Genome accession   NZ_CP058321
Coordinates   328402..329445 (+) Length   347 a.a.
NCBI ID   WP_186254598.1    Uniprot ID   A0A7G7Z4S4
Organism   Halomonas sp. SH5A2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 323402..334445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HXW73_RS01585 (HXW73_01590) sfsA 323543..324334 (+) 792 WP_186254593.1 DNA/RNA nuclease SfsA -
  HXW73_RS01590 (HXW73_01595) metW 324281..324874 (-) 594 WP_186255874.1 methionine biosynthesis protein MetW -
  HXW73_RS01595 (HXW73_01600) - 324874..326070 (-) 1197 WP_186254594.1 homoserine O-acetyltransferase -
  HXW73_RS01600 (HXW73_01605) - 326127..326720 (-) 594 WP_186254595.1 YggT family protein -
  HXW73_RS01605 (HXW73_01610) proC 326751..327575 (-) 825 WP_186254596.1 pyrroline-5-carboxylate reductase -
  HXW73_RS01610 (HXW73_01615) - 327664..328368 (-) 705 WP_186254597.1 YggS family pyridoxal phosphate-dependent enzyme -
  HXW73_RS01615 (HXW73_01620) pilT 328402..329445 (+) 1044 WP_186254598.1 type IV pilus twitching motility protein PilT Machinery gene
  HXW73_RS01620 (HXW73_01625) pilU 329449..330615 (+) 1167 WP_186254599.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HXW73_RS01625 (HXW73_01630) - 330626..331756 (-) 1131 WP_186254600.1 anhydro-N-acetylmuramic acid kinase -
  HXW73_RS01630 (HXW73_01635) tyrS 331871..333070 (+) 1200 WP_186254601.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38040.50 Da        Isoelectric Point: 6.1335

>NTDB_id=463752 HXW73_RS01615 WP_186254598.1 328402..329445(+) (pilT) [Halomonas sp. SH5A2]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMDNSEVRRLIHDIMNDRQRRDYEEHLEVDFSFEVPGVAR
FRVNAFNQARGAGAVFRTIPNQVLSMQTLGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRFEHILTIED
PVEFVHASKRCLINQREVHRDTHSFTEALRSALREDPDVILVGELRDQETIRLALTAAETGHLVLGTLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESLQAVVSQVLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQIFSAIQTGGGLGMQTLDAAL
AQLVKEGNVGMEDAQALAKGTLASREA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=463752 HXW73_RS01615 WP_186254598.1 328402..329445(+) (pilT) [Halomonas sp. SH5A2]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAATGCATCCGACCTGCACTTATCCGCTGGTTTGCCGCCCAT
GATACGGGTTGATGGCGATATCCGTCGGCTGAATGTGCCCGCCATGGACAACAGCGAGGTGCGTCGGTTGATTCACGACA
TCATGAACGATCGCCAGCGTCGCGACTACGAAGAGCACCTCGAAGTCGACTTTTCTTTCGAAGTGCCGGGCGTGGCGCGT
TTTCGGGTCAACGCCTTTAATCAGGCGCGCGGTGCCGGGGCGGTATTTCGCACCATTCCCAACCAGGTGCTATCCATGCA
GACCCTTGGGTTGGGGGAGGTGTTCGAGCGCCTTGCCATGCTGCCGCGCGGCCTGGTGTTGGTGACCGGGCCAACCGGTT
CGGGCAAAAGCACCACGCTTGCGGCGATGATCGATTACATCAACGATCATCGCTTTGAGCACATTTTAACCATCGAAGAC
CCGGTTGAGTTCGTGCATGCCAGCAAGCGTTGCCTGATTAATCAGCGGGAAGTCCATCGTGACACGCATAGCTTTACAGA
GGCGTTGCGCAGCGCGCTACGCGAAGACCCGGATGTGATTCTGGTGGGAGAGCTTCGCGATCAGGAAACCATCCGTCTGG
CATTAACGGCGGCAGAGACCGGCCATCTGGTGCTGGGGACGCTGCATACCACGTCGGCGGCCAAGACCATTGACCGGATT
ATTGATGTCTTTCCTGGTGAAGAGAAAGCCATGGTGCGTTCGATGCTGTCTGAGTCGCTGCAGGCGGTGGTATCCCAGGT
GCTATTGAAGCGCCAGGGGGGCGGCCGTGTGGCGGCCCATGAAATTCTGATCGCGACCTCGGCGGTGCGTAATCTGATTC
GTGAAGACAAGGTGGCGCAGATCTTCTCGGCGATTCAAACCGGCGGCGGCCTGGGCATGCAAACGTTGGACGCCGCACTG
GCGCAACTGGTCAAAGAGGGTAATGTCGGCATGGAAGACGCCCAGGCGCTGGCCAAGGGCACTCTCGCTTCGCGGGAGGC
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G7Z4S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.991

97.695

0.752
  pilT Pseudomonas stutzeri DSM 10701

76.696

97.695

0.749
  pilT Acinetobacter baylyi ADP1

75.516

97.695

0.738
  pilT Acinetobacter nosocomialis M2

74.926

97.695

0.732
  pilT Acinetobacter baumannii D1279779

74.926

97.695

0.732
  pilT Acinetobacter baumannii strain A118

74.926

97.695

0.732
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.536

96.83

0.683
  pilT Vibrio cholerae strain A1552

70.536

96.83

0.683
  pilT Legionella pneumophila strain Lp02

69.822

97.406

0.68
  pilT Legionella pneumophila strain ERS1305867

69.822

97.406

0.68
  pilT Neisseria meningitidis 8013

66.667

98.559

0.657
  pilT Neisseria gonorrhoeae MS11

66.374

98.559

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.521

93.948

0.522
  pilU Pseudomonas stutzeri DSM 10701

43.284

96.542

0.418
  pilU Vibrio cholerae strain A1552

41.493

96.542

0.401
  pilU Acinetobacter baylyi ADP1

41.071

96.83

0.398