Detailed information NTG0463753

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HXW73_RS01620
• Genome accession: NZ_CP058321
• Coordinates: 329449..330615 (+)
• Length: 388 a.a.
• NCBI ID: WP_186254599.1
• Uniprot ID: A0A7G7Z4S5
• Organism: Halomonas sp. SH5A2
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 324449..335615

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HXW73_RS01595 (HXW73_01600)	-	324874..326070 (-)	1197	WP_186254594.1	homoserine O-acetyltransferase	-
HXW73_RS01600 (HXW73_01605)	-	326127..326720 (-)	594	WP_186254595.1	YggT family protein	-
HXW73_RS01605 (HXW73_01610)	proC	326751..327575 (-)	825	WP_186254596.1	pyrroline-5-carboxylate reductase	-
HXW73_RS01610 (HXW73_01615)	-	327664..328368 (-)	705	WP_186254597.1	YggS family pyridoxal phosphate-dependent enzyme	-
HXW73_RS01615 (HXW73_01620)	pilT	328402..329445 (+)	1044	WP_186254598.1	type IV pilus twitching motility protein PilT	Machinery gene
HXW73_RS01620 (HXW73_01625)	pilU	329449..330615 (+)	1167	WP_186254599.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HXW73_RS01625 (HXW73_01630)	-	330626..331756 (-)	1131	WP_186254600.1	anhydro-N-acetylmuramic acid kinase	-
HXW73_RS01630 (HXW73_01635)	tyrS	331871..333070 (+)	1200	WP_186254601.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 43041.90 Da
• Isoelectric Point: 6.2987

>NTDB_id=463753 HXW73_RS01620 WP_186254599.1 329449..330615(+) (pilU) [Halomonas sp. SH5A2]
MANNDVSTLTPGQWLHQLLDIMVTQQASDLLISTGAPPSLKVSSGLVPLGQQPLSEHQVSELVTHTVPEGLRERFSQQRE
ANFALGFEDKGRFRVSAFQQRNQPAMVIRRIEQHIPTLETLGVPTTLASLAHAKRGLVLVVGGTGTGKSTTLAAMVQERN
ETVGGHIISIEDPIEYLHPHKRAIVNQREVGIDTESFEVALKNTLRQAPDVILIGEIRSRETMEHALTFSETGHLCLATL
HANNANQALERIIHFFPHERHEQIWMDLSLNLRAVVAQQLLPSLNGGRCAAVEIMLQSSRIADLIRKGRVDEMKAAMAAS
RDDGMQTFDQALFDLFQASQISQEVALAHADSTNDLRLMLKLQGEATSRPLDERAPSHWTLRDRDDAW

Nucleotide

• Length: 1167 bp

>NTDB_id=463753 HXW73_RS01620 WP_186254599.1 329449..330615(+) (pilU) [Halomonas sp. SH5A2]
ATGGCGAACAATGACGTCTCAACGCTGACGCCTGGCCAGTGGCTGCATCAGCTGCTGGATATCATGGTGACGCAGCAAGC
TTCAGACCTGCTCATTTCAACGGGCGCGCCGCCCAGCCTCAAGGTGTCCAGCGGGCTGGTTCCCCTGGGCCAACAGCCCT
TGAGTGAACATCAGGTCAGTGAGCTGGTAACACACACCGTGCCTGAAGGGCTGCGCGAGCGTTTCAGTCAACAGCGCGAG
GCGAATTTTGCGCTGGGGTTTGAAGACAAGGGGCGCTTTCGCGTTAGCGCCTTCCAGCAGAGAAACCAGCCCGCCATGGT
GATACGCCGTATTGAGCAACACATTCCTACCCTGGAAACATTGGGTGTGCCGACGACGCTTGCCTCTCTTGCCCATGCCA
AACGGGGGTTGGTGCTGGTGGTGGGTGGCACGGGTACCGGAAAATCCACCACCCTGGCGGCGATGGTGCAGGAACGTAAT
GAAACGGTCGGTGGACACATCATCAGCATTGAAGATCCGATCGAATACCTGCACCCGCACAAACGCGCCATCGTTAACCA
GCGTGAAGTGGGCATTGATACCGAGTCATTTGAAGTAGCGCTCAAGAACACCCTGCGCCAAGCCCCGGATGTGATCCTGA
TCGGTGAGATTCGCAGCCGTGAAACCATGGAACACGCCTTGACGTTCTCTGAAACCGGGCATCTCTGCCTGGCCACCTTG
CACGCCAACAACGCCAATCAGGCGTTGGAGCGGATCATCCATTTCTTTCCCCATGAGCGACATGAGCAGATATGGATGGA
TCTTTCCCTCAACCTCCGCGCGGTGGTGGCTCAACAGCTGTTGCCCAGCCTGAACGGTGGTCGTTGTGCGGCGGTTGAAA
TCATGCTGCAGTCGTCACGGATTGCCGACTTGATTCGTAAAGGCAGGGTCGACGAGATGAAAGCCGCCATGGCGGCATCG
CGTGATGACGGCATGCAGACTTTTGACCAAGCGCTCTTCGATCTTTTTCAGGCATCACAGATCAGTCAGGAGGTGGCGCT
TGCCCACGCGGACTCCACCAATGACTTGCGGCTCATGCTGAAGCTGCAGGGGGAGGCGACGTCTCGCCCGCTGGATGAGC
GCGCTCCGAGCCATTGGACCCTGCGCGACCGCGACGATGCATGGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (24-282)

3D structure

• AlphaFold DB: A0A7G7Z4S5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Vibrio cholerae strain A1552	61.207	89.691	0.549
pilU	Pseudomonas stutzeri DSM 10701	60.227	90.722	0.546
pilU	Acinetobacter baylyi ADP1	55.801	93.299	0.521
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.44	88.402	0.384