Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VY92_RS01320 Genome accession   NZ_CP058307
Coordinates   219150..220574 (+) Length   474 a.a.
NCBI ID   WP_035687022.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain AVPG2015     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 214150..225574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VY92_RS01290 (VY92_001290) lgt 214725..215525 (-) 801 WP_035687035.1 prolipoprotein diacylglyceryl transferase -
  VY92_RS01295 (VY92_001295) - 215535..216326 (-) 792 WP_035687031.1 sulfite exporter TauE/SafE family protein -
  VY92_RS01300 (VY92_001300) rppH 216329..216922 (-) 594 WP_035687029.1 RNA pyrophosphohydrolase -
  VY92_RS01305 (VY92_001305) - 217494..217913 (-) 420 WP_124994872.1 hypothetical protein -
  VY92_RS01310 (VY92_001310) ampD 218012..218566 (-) 555 WP_035687059.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VY92_RS01315 (VY92_001315) pilA 218706..219137 (+) 432 WP_035687024.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VY92_RS01320 (VY92_001320) pilB 219150..220574 (+) 1425 WP_035687022.1 GspE/PulE family protein Machinery gene
  VY92_RS01325 (VY92_001325) pyrH 220824..221540 (+) 717 WP_035687020.1 UMP kinase -
  VY92_RS01330 (VY92_001330) frr 221565..222122 (+) 558 WP_017807054.1 ribosome recycling factor -
  VY92_RS01335 (VY92_001335) ispC 222302..223498 (+) 1197 WP_035687018.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
  VY92_RS01340 (VY92_001340) uppS 223652..224380 (+) 729 WP_035687015.1 polyprenyl diphosphate synthase -
  VY92_RS01345 (VY92_001345) - 224384..225247 (+) 864 WP_035687012.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 53762.51 Da        Isoelectric Point: 5.2146

>NTDB_id=463625 VY92_RS01320 WP_035687022.1 219150..220574(+) (pilB) [Avibacterium paragallinarum strain AVPG2015]
MAEYSLDEDDIVYAVDGKCFEITPHLWQQNKQQKDILIRYLAVPLQEDEHTLWLGIDDLTNLSACESFSFLTGKIVEPIL
LSEQDLKSLLQKLSPTLVEEPALLDFYQSSISPYSENQPEKLDEPIIQILNKVFESAMQLNASDIHLEPQNEQLQIRFRI
DGVLQHQPPISKQMSQRLISRLKLLARLDLSETRLPQDGRFHFKTTFSDILDFRISTLPTYYGEKAVLRLQKNKPVTLDF
SELGMTAIQRKTLQDALSQPQGLILVTGPTGSGKSITLYTALEWLNQSEKHILTAEDPIEIELPGIIQTQINPQIGLDFN
RLLRTFLRQDPDIIMLGEIRDAESAAIALRAAQTGHLVLSTLHTNNAHSAIARLLQLGIQQYEIESCLLLVIAQRLVRKK
CLHKTKNCECYQGYSGRTGIYQFLQAIFDSNTGQISYQTDYPSLWDSASHKIAQGITDIAELERVLGRHTKAEL

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=463625 VY92_RS01320 WP_035687022.1 219150..220574(+) (pilB) [Avibacterium paragallinarum strain AVPG2015]
ATGGCTGAGTATTCTTTAGACGAGGACGACATTGTATATGCTGTTGATGGAAAATGCTTTGAAATAACACCGCACTTATG
GCAACAGAATAAGCAGCAAAAAGATATTTTAATACGTTATTTAGCAGTACCTTTACAGGAGGATGAACATACACTTTGGC
TTGGTATTGATGACTTAACAAATCTATCGGCTTGTGAAAGTTTTTCATTTCTAACAGGAAAAATAGTTGAGCCGATTTTG
TTATCAGAACAAGATTTAAAATCGCTATTACAGAAGTTATCTCCCACATTAGTAGAAGAGCCGGCATTACTTGATTTTTA
TCAATCGTCTATTTCACCTTATAGCGAAAATCAACCAGAAAAACTTGATGAACCAATTATTCAAATACTAAATAAAGTTT
TTGAATCGGCAATGCAATTAAATGCCTCAGATATTCATTTAGAGCCACAAAATGAGCAATTGCAGATCCGTTTTCGTATT
GACGGGGTATTGCAACATCAGCCTCCTATTTCTAAACAAATGAGCCAACGTTTGATTTCTCGTTTAAAACTTCTAGCTCG
TCTTGATCTTAGCGAAACTCGCTTACCACAAGATGGAAGATTTCATTTCAAAACGACATTTTCCGATATATTAGACTTCC
GTATATCGACATTACCTACATATTATGGAGAAAAAGCGGTACTACGTTTACAAAAAAATAAACCTGTTACCTTGGATTTT
TCTGAGCTGGGGATGACAGCGATACAACGAAAAACATTACAAGATGCTTTATCTCAACCTCAAGGACTCATTTTAGTAAC
GGGGCCAACTGGGAGTGGGAAAAGTATTACGCTCTATACAGCATTAGAATGGCTTAATCAATCGGAAAAACATATTCTAA
CAGCAGAAGATCCGATTGAAATAGAACTTCCAGGGATTATCCAAACGCAAATTAACCCACAAATTGGTTTAGATTTTAAC
CGTTTGTTGCGTACATTTTTACGTCAGGATCCAGACATTATTATGCTTGGTGAGATCCGTGATGCAGAAAGTGCAGCGAT
TGCGTTACGTGCAGCACAAACTGGACATTTAGTCCTTTCAACATTACATACAAATAATGCACATTCAGCTATTGCTCGCT
TGTTACAGCTTGGTATTCAACAATATGAAATTGAAAGTTGTTTATTATTAGTGATTGCCCAGCGTTTAGTCCGTAAAAAA
TGTCTACACAAAACGAAAAATTGCGAATGTTATCAAGGTTATAGTGGGCGTACTGGTATTTATCAATTTTTACAAGCTAT
TTTTGATTCAAATACAGGGCAAATTTCTTATCAAACCGATTATCCCTCATTATGGGATAGTGCTTCACATAAAATAGCAC
AAGGTATCACAGATATTGCTGAATTAGAACGAGTTTTAGGTAGGCATACAAAAGCTGAACTATAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(132-399)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

64.693

99.789

0.646
  pilB Haemophilus influenzae 86-028NP

63.924

100

0.639
  pilB Glaesserella parasuis strain SC1401

57.263

100

0.574
  pilB Legionella pneumophila strain ERS1305867

39.634

100

0.411
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.411
  pilB Vibrio parahaemolyticus RIMD 2210633

39.794

100

0.407
  pilB Vibrio cholerae strain A1552

39.419

100

0.401
  pilB Acinetobacter baylyi ADP1

37.626

100

0.395
  pilF Neisseria gonorrhoeae MS11

37.317

100

0.376
  pilB Acinetobacter baumannii D1279779

36.495

100

0.373