Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CQZ96_RS09420 Genome accession   NZ_CP058272
Coordinates   1993363..1994400 (+) Length   345 a.a.
NCBI ID   WP_004722735.1    Uniprot ID   A0A077KW48
Organism   Acinetobacter sp. MYb10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1988363..1999400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CQZ96_RS09400 (CQZ96_009410) - 1989228..1990019 (-) 792 WP_004722726.1 hypothetical protein -
  CQZ96_RS09405 (CQZ96_009415) - 1990316..1991119 (-) 804 WP_004722728.1 thioesterase family protein -
  CQZ96_RS09410 (CQZ96_009420) - 1991325..1992157 (-) 833 Protein_1859 undecaprenyl-diphosphate phosphatase -
  CQZ96_RS09415 (CQZ96_009425) - 1992280..1992972 (-) 693 WP_004722732.1 YggS family pyridoxal phosphate-dependent enzyme -
  CQZ96_RS09420 (CQZ96_009430) pilT 1993363..1994400 (+) 1038 WP_004722735.1 type IV pilus twitching motility protein PilT Machinery gene
  CQZ96_RS09425 (CQZ96_009435) pilU 1994426..1995544 (+) 1119 WP_004722736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CQZ96_RS09430 (CQZ96_009440) fur 1995624..1996061 (-) 438 WP_004722737.1 ferric iron uptake transcriptional regulator -
  CQZ96_RS09435 (CQZ96_009445) - 1996174..1996572 (+) 399 WP_004722739.1 outer membrane protein assembly factor BamE -
  CQZ96_RS09440 (CQZ96_009450) - 1996657..1996986 (-) 330 WP_035371671.1 RnfH family protein -
  CQZ96_RS09445 (CQZ96_009455) - 1996995..1998062 (-) 1068 WP_105713222.1 hypothetical protein -
  CQZ96_RS09450 (CQZ96_009460) - 1998281..1998736 (-) 456 WP_056514616.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38286.02 Da        Isoelectric Point: 6.5057

>NTDB_id=463347 CQZ96_RS09420 WP_004722735.1 1993363..1994400(+) (pilT) [Acinetobacter sp. MYb10]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHRLVYDIMNDKQRRDFEEQLETDFSFEVPGIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDLCELPRGLILVTGPTGSGKSTTLASMIDFINDSRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVAKGVISPQVARNVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=463347 CQZ96_RS09420 WP_004722735.1 1993363..1994400(+) (pilT) [Acinetobacter sp. MYb10]
ATGGATATTACAGAATTATTGGCGTTTTCCGTCAAAAACGGTGCATCTGACTTACACCTTTCAGCAGGTATGCCACCGAT
GATTCGTGTCGATGGTGAGGTCCGTCGTATCAATTTACCAGCACTGGAACATAAAGAAGTTCACAGACTGGTTTATGACA
TCATGAATGATAAACAACGTCGTGATTTTGAAGAACAATTAGAAACTGACTTCTCATTTGAAGTTCCAGGTATTGCCCGT
TTCCGTGTCAACGCATTCAACCAAAACCGTGGTGCGGGTGCTGTATTTCGTACCATTCCTTCCAAAGTATTGACCATGGA
AGATTTAGGTTTAGGACAAATCTTTAAGGATCTTTGTGAGTTACCCCGTGGTTTGATCCTTGTAACAGGTCCAACAGGTT
CCGGTAAATCAACCACATTGGCCTCTATGATTGACTTTATTAATGACAGTCGCTATGACCATATTTTAACGGTCGAAGAT
CCAATCGAATTTGTACACCAATCTAAAAAGTGCCTTATCAACCAACGTGAAGTGCATCGAGATACACATGGCTTTAATGA
AGCACTGCGTTCTGCATTACGTGAAGACCCTGACATTATCTTGGTTGGTGAGATGCGTGACCTTGAAACCATTCGCCTGG
CTTTAACTGCAGCAGAAACAGGTCACTTGGTTTTCGGTACACTACACACCACATCAGCGGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTCCGTGCCATGTTATCAGAATCATTACAAGCGGTTATTTCACAGAC
CCTGCTGAAAAAGAATGGTGGCGGACGAGTTGCAGCACATGAAATCATGATTGGTATTCCTGCAATTCGTAACTTGATTC
GTGAAAACAAAGTTGCACAGATGTATTCAGCAATTCAAACCGGTGCAAACTATGGTATGACCACATTGGATCAGAGTTTA
AAAACACTGGTCGCCAAAGGTGTTATTAGTCCTCAAGTTGCAAGAAATGTAGCAAAACAACCAGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A077KW48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

93.913

100

0.939
  pilT Acinetobacter baumannii D1279779

93.913

100

0.939
  pilT Acinetobacter nosocomialis M2

93.623

100

0.936
  pilT Acinetobacter baylyi ADP1

86.628

99.71

0.864
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

82.849

99.71

0.826
  pilT Legionella pneumophila strain Lp02

75.444

97.971

0.739
  pilT Legionella pneumophila strain ERS1305867

75.444

97.971

0.739
  pilT Neisseria meningitidis 8013

66.957

100

0.67
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.277

96.232

0.667
  pilT Vibrio cholerae strain A1552

69.277

96.232

0.667
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.516
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Acinetobacter baylyi ADP1

39.884

100

0.4
  pilU Pseudomonas stutzeri DSM 10701

41.39

95.942

0.397