Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CQZ96_RS09425 Genome accession   NZ_CP058272
Coordinates   1994426..1995544 (+) Length   372 a.a.
NCBI ID   WP_004722736.1    Uniprot ID   A0A077KUG3
Organism   Acinetobacter sp. MYb10     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1989426..2000544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CQZ96_RS09405 (CQZ96_009415) - 1990316..1991119 (-) 804 WP_004722728.1 thioesterase family protein -
  CQZ96_RS09410 (CQZ96_009420) - 1991325..1992157 (-) 833 Protein_1859 undecaprenyl-diphosphate phosphatase -
  CQZ96_RS09415 (CQZ96_009425) - 1992280..1992972 (-) 693 WP_004722732.1 YggS family pyridoxal phosphate-dependent enzyme -
  CQZ96_RS09420 (CQZ96_009430) pilT 1993363..1994400 (+) 1038 WP_004722735.1 type IV pilus twitching motility protein PilT Machinery gene
  CQZ96_RS09425 (CQZ96_009435) pilU 1994426..1995544 (+) 1119 WP_004722736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CQZ96_RS09430 (CQZ96_009440) fur 1995624..1996061 (-) 438 WP_004722737.1 ferric iron uptake transcriptional regulator -
  CQZ96_RS09435 (CQZ96_009445) - 1996174..1996572 (+) 399 WP_004722739.1 outer membrane protein assembly factor BamE -
  CQZ96_RS09440 (CQZ96_009450) - 1996657..1996986 (-) 330 WP_035371671.1 RnfH family protein -
  CQZ96_RS09445 (CQZ96_009455) - 1996995..1998062 (-) 1068 WP_105713222.1 hypothetical protein -
  CQZ96_RS09450 (CQZ96_009460) - 1998281..1998736 (-) 456 WP_056514616.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41869.16 Da        Isoelectric Point: 6.2461

>NTDB_id=463348 CQZ96_RS09425 WP_004722736.1 1994426..1995544(+) (pilU) [Acinetobacter sp. MYb10]
MDFNDLLNMMIEQKASDLFITADVEPSMKINGQIVPVVKTKLNGEIIMQLLNSIMSEKQRKEFAETRECNFAITNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFESDRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLQIKLDEEGGAQLLNSNSKLTFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=463348 CQZ96_RS09425 WP_004722736.1 1994426..1995544(+) (pilU) [Acinetobacter sp. MYb10]
ATGGACTTTAATGACTTGCTCAACATGATGATTGAGCAAAAAGCATCAGACTTATTTATTACCGCGGATGTTGAACCATC
AATGAAAATTAATGGTCAAATTGTTCCTGTGGTAAAAACCAAGTTAAACGGCGAAATTATTATGCAATTGCTTAACTCTA
TTATGAGTGAAAAGCAGCGCAAAGAATTTGCTGAGACGCGTGAGTGTAACTTTGCCATTACCAATCGCGATAAAACTGCG
CGTTTCCGTGTCAGCGCTTTTCAACAACGTGATATGCCTGGAATGGTTTTGCGTCGTATTGAAACTCAAATTCCGACCAT
GGATGAGCTTAAATTACCGCCTATCCTTAAAGATCTCGCAATGACCAAACGTGGGATCATTATCTTTGTTGGTGCAACAG
GGACAGGTAAATCAACTTCGCTTGCATCCATCATTGGTTATCGCAATCAAAATTCTAAAGGTCATATCATCACCATTGAA
GACCCAATTGAATTTGTACACGAACATGCAGGATGTATTATTACCCAACGTGAAGTGGGAATTGATACAGATTCTTTTGA
AATTGCACTTAAGAATACTTTGCGTCAAGCCCCAGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAACAATGGACT
ATGCCATCGCTTTTGCTGAAACAGGTCACTTAGTCTTTGCCACACTGCATGCCAACAACGCCAACCAAGCAATTGACCGA
ATTATTCACTTCTTTGAGTCTGACCGTCATAGCCAGTTGTTTATGGATCTATCACTTAACCTCAAAGCCATGGTTGCGCA
ACAATTGATTCCAACACCAGACGGTAATTCTCGTCGTGCTGCAATTGAAATTCTCATCAACTCACCGTTAGTTTCAGACT
TAATCCGTAAAGGTGATGTACATGAAATTAAGGATTTGATGAAGCGCTCTCGTGAATTAGGTATGCAGACATTTGACCAA
GCTTTATTTGACCTCTATAAAGCGGGACAAATTACCTATAAAGACGCACTTAAACATGCTGATTCTCCAAATGATTTACG
TCTACAAATCAAATTGGATGAAGAAGGTGGTGCACAGCTACTCAATTCAAATAGCAAACTCACCTTTGATGGACAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A077KUG3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.455

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

69.126

98.387

0.68
  pilU Vibrio cholerae strain A1552

54.595

99.462

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.353

93.011

0.403
  pilT Legionella pneumophila strain Lp02

40.828

90.86

0.371
  pilT Legionella pneumophila strain ERS1305867

40.828

90.86

0.371
  pilT Acinetobacter baumannii D1279779

39.65

92.204

0.366
  pilT Acinetobacter baylyi ADP1

39.65

92.204

0.366
  pilT Acinetobacter nosocomialis M2

39.65

92.204

0.366
  pilT Acinetobacter baumannii strain A118

39.65

92.204

0.366
  pilT Pseudomonas aeruginosa PAK

39.306

93.011

0.366
  pilT Neisseria meningitidis 8013

39.412

91.398

0.36
  pilT Neisseria gonorrhoeae MS11

39.412

91.398

0.36