Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HWV02_RS01710 Genome accession   NZ_CP056121
Coordinates   364682..365716 (+) Length   344 a.a.
NCBI ID   WP_211678209.1    Uniprot ID   -
Organism   Moritella sp. 28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 359682..370716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWV02_RS01680 (HWV02_01680) hemW 359991..361127 (-) 1137 WP_211675605.1 radical SAM family heme chaperone HemW -
  HWV02_RS01685 (HWV02_01685) - 361178..361792 (-) 615 WP_211689538.1 XTP/dITP diphosphatase -
  HWV02_RS01690 (HWV02_01690) - 361928..362356 (-) 429 WP_211678205.1 DUF4426 domain-containing protein -
  HWV02_RS01695 (HWV02_01695) - 362474..363019 (-) 546 WP_211689539.1 YggT family protein -
  HWV02_RS01700 (HWV02_01700) proC 363072..363899 (-) 828 WP_211689540.1 pyrroline-5-carboxylate reductase -
  HWV02_RS01705 (HWV02_01705) - 363956..364651 (-) 696 WP_211689541.1 YggS family pyridoxal phosphate-dependent enzyme -
  HWV02_RS01710 (HWV02_01710) pilT 364682..365716 (+) 1035 WP_211678209.1 type IV pilus twitching motility protein PilT Machinery gene
  HWV02_RS01715 (HWV02_01715) pilU 365728..366843 (+) 1116 WP_304610881.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HWV02_RS01720 (HWV02_01720) yaaA 366907..367686 (+) 780 WP_211678210.1 peroxide stress protein YaaA -
  HWV02_RS01725 (HWV02_01725) - 367842..368489 (-) 648 WP_211689542.1 TIGR04211 family SH3 domain-containing protein -
  HWV02_RS01730 (HWV02_01730) - 368649..369923 (-) 1275 WP_211678212.1 inorganic phosphate transporter -
  HWV02_RS01735 (HWV02_01735) - 369947..370627 (-) 681 WP_211678213.1 TIGR00153 family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38535.12 Da        Isoelectric Point: 6.4520

>NTDB_id=458236 HWV02_RS01710 WP_211678209.1 364682..365716(+) (pilT) [Moritella sp. 28]
MDVTELLTFSVKHNASDLHLSSGVPPMIRVDGDVRKINLPAFDAKQVHALIYDIMDDKQRKDYEEHLEVDFSFEINNVAR
FRVNAFNQHRGPAATFRTIPSEIKSMAELSVPDIFHDLVELPRGLVLVTGPTGSGKSTTLAAMVDHINEHQHKHILTIED
PIEFVHQNKKSLINQREVFRDTKSFNAALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPGQEKDMVRSMLSESLRAVISQTLLKRTGGGRVAAHEIMLGTPAIRNLIREDKVAQMYSVIQTGMMHGMQTLDQSL
RELVNMGIVTSEEARIRAVDQQSF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=458236 HWV02_RS01710 WP_211678209.1 364682..365716(+) (pilT) [Moritella sp. 28]
ATGGATGTTACAGAGTTACTTACATTTAGTGTAAAGCATAACGCATCAGATCTACACCTTTCTTCGGGTGTTCCTCCGAT
GATTCGTGTGGACGGTGATGTACGTAAAATCAATTTACCTGCTTTTGACGCAAAACAAGTACATGCGCTAATTTATGACA
TTATGGATGATAAACAGCGTAAAGACTATGAAGAACATTTGGAAGTCGATTTTTCTTTTGAAATTAATAACGTTGCTCGT
TTTCGTGTGAATGCATTTAATCAACATCGTGGTCCAGCCGCAACGTTTCGTACTATTCCAAGTGAAATTAAAAGCATGGC
AGAATTAAGTGTGCCAGATATTTTCCATGATTTGGTGGAACTGCCACGTGGTTTAGTATTAGTGACCGGACCAACGGGAT
CTGGTAAGTCAACGACACTGGCTGCGATGGTTGATCATATTAACGAGCATCAGCATAAACATATTCTGACAATCGAAGAT
CCGATTGAATTTGTACATCAAAATAAAAAAAGCTTAATTAATCAGCGTGAAGTATTTCGGGATACCAAGAGCTTTAATGC
CGCATTACGGTCTGCATTACGTGAAGATCCCGATATTATTTTGGTCGGTGAAATGCGTGATTTAGAAACCATCCGTTTGG
CATTAACCGCCGCTGAAACGGGGCATTTGGTCTTTGGTACTTTGCATACTACCTCGGCAGCAAAAACCATTGACCGTATT
GTTGATGTATTCCCAGGGCAAGAGAAGGATATGGTTCGTTCGATGCTGTCGGAATCATTACGTGCGGTAATTTCCCAGAC
ATTATTAAAACGTACCGGTGGCGGTCGAGTTGCGGCACATGAAATCATGCTTGGTACACCAGCTATTCGTAATTTGATCC
GTGAGGATAAAGTTGCGCAGATGTATTCCGTGATCCAAACGGGTATGATGCATGGCATGCAAACATTAGATCAGTCGTTA
CGTGAATTGGTTAATATGGGTATTGTCACGAGCGAAGAAGCGCGCATTCGAGCTGTTGATCAACAAAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

75.581

100

0.756
  pilT Acinetobacter baumannii D1279779

75.581

100

0.756
  pilT Acinetobacter baumannii strain A118

75.581

100

0.756
  pilT Acinetobacter baylyi ADP1

75.291

100

0.753
  pilT Pseudomonas stutzeri DSM 10701

74.709

100

0.747
  pilT Pseudomonas aeruginosa PAK

74.419

100

0.744
  pilT Legionella pneumophila strain Lp02

71.221

100

0.712
  pilT Legionella pneumophila strain ERS1305867

71.221

100

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.014

100

0.712
  pilT Vibrio cholerae strain A1552

71.014

100

0.712
  pilT Neisseria meningitidis 8013

68.406

100

0.686
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Vibrio cholerae strain A1552

41.231

94.477

0.39
  pilU Acinetobacter baylyi ADP1

40.426

95.64

0.387
  pilU Pseudomonas stutzeri DSM 10701

38.348

98.547

0.378