Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HWV02_RS01715 Genome accession   NZ_CP056121
Coordinates   365728..366843 (+) Length   371 a.a.
NCBI ID   WP_304610881.1    Uniprot ID   -
Organism   Moritella sp. 28     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 360728..371843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWV02_RS01685 (HWV02_01685) - 361178..361792 (-) 615 WP_211689538.1 XTP/dITP diphosphatase -
  HWV02_RS01690 (HWV02_01690) - 361928..362356 (-) 429 WP_211678205.1 DUF4426 domain-containing protein -
  HWV02_RS01695 (HWV02_01695) - 362474..363019 (-) 546 WP_211689539.1 YggT family protein -
  HWV02_RS01700 (HWV02_01700) proC 363072..363899 (-) 828 WP_211689540.1 pyrroline-5-carboxylate reductase -
  HWV02_RS01705 (HWV02_01705) - 363956..364651 (-) 696 WP_211689541.1 YggS family pyridoxal phosphate-dependent enzyme -
  HWV02_RS01710 (HWV02_01710) pilT 364682..365716 (+) 1035 WP_211678209.1 type IV pilus twitching motility protein PilT Machinery gene
  HWV02_RS01715 (HWV02_01715) pilU 365728..366843 (+) 1116 WP_304610881.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HWV02_RS01720 (HWV02_01720) yaaA 366907..367686 (+) 780 WP_211678210.1 peroxide stress protein YaaA -
  HWV02_RS01725 (HWV02_01725) - 367842..368489 (-) 648 WP_211689542.1 TIGR04211 family SH3 domain-containing protein -
  HWV02_RS01730 (HWV02_01730) - 368649..369923 (-) 1275 WP_211678212.1 inorganic phosphate transporter -
  HWV02_RS01735 (HWV02_01735) - 369947..370627 (-) 681 WP_211678213.1 TIGR00153 family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40805.02 Da        Isoelectric Point: 6.2693

>NTDB_id=458237 HWV02_RS01715 WP_304610881.1 365728..366843(+) (pilU) [Moritella sp. 28]
MGMLPQLFSAMKKLTGSDMYISAGIAPTVKVHGSLRALSEHKLTAEQSLALVKEAMSEDEYASFCRTKESNFGIFMPDIG
RFRVSAFWQLEKAGMVIRRIETDIPAMDELHLPPILKHTALEKRGLVLVVGATGSGKSTTQAAMVGYRNQNSSGHILTIE
DPIEFVHEHGSCIVTQREVGIDTESFEDALKSSLRQAPDVILIGEIRSQETMEFALAFAETGHLCMATLHATNANQAIDR
ILHLVPKEKHAQLLYDLSLNLKAIVAQQLIPVQGGSSRRGAFEILLNTPLVSELIRKNELHKIKEVMTKSGELGMQTFDQ
ALLALYNTNMISYTEALHHADSPNDLRLMIKLNQGQAGSDSMLDGVTIEAL

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=458237 HWV02_RS01715 WP_304610881.1 365728..366843(+) (pilU) [Moritella sp. 28]
ATGGGGATGCTACCACAACTCTTTAGTGCGATGAAAAAGCTTACTGGTTCTGACATGTATATTTCGGCGGGTATTGCACC
CACAGTGAAAGTACATGGCTCATTACGTGCGCTCTCGGAACATAAATTAACGGCTGAGCAATCATTAGCATTGGTGAAAG
AAGCCATGTCTGAGGATGAATATGCTTCTTTTTGCCGTACCAAAGAATCTAATTTCGGTATTTTCATGCCGGATATTGGT
CGTTTCCGTGTCAGCGCATTTTGGCAATTAGAAAAAGCGGGCATGGTGATCCGTCGTATTGAAACCGATATACCTGCAAT
GGATGAGTTACACTTGCCACCAATATTAAAACACACAGCGCTAGAGAAGCGTGGTTTAGTGTTGGTTGTTGGGGCGACGG
GTTCTGGTAAATCAACCACGCAAGCAGCCATGGTGGGTTATCGTAACCAAAACTCATCAGGTCATATCTTAACGATTGAA
GATCCGATTGAGTTTGTCCATGAACATGGTAGTTGTATTGTTACTCAGCGTGAAGTAGGAATTGATACCGAGTCTTTTGA
AGATGCGCTGAAGAGCTCATTACGTCAAGCGCCAGATGTGATCTTGATTGGTGAAATCCGCTCGCAAGAAACGATGGAGT
TTGCCTTGGCGTTTGCGGAAACGGGGCATTTATGTATGGCAACGCTGCATGCAACTAATGCCAATCAGGCTATCGATCGT
ATTTTGCACTTAGTACCAAAAGAAAAGCATGCTCAGTTGTTATATGATTTGTCACTAAATTTAAAAGCCATTGTGGCGCA
GCAATTAATCCCAGTACAAGGTGGTTCAAGCCGGCGTGGTGCATTTGAAATTTTATTGAATACACCTTTGGTCAGTGAAT
TGATCCGCAAAAATGAACTGCATAAAATTAAAGAAGTGATGACAAAATCGGGCGAGCTGGGAATGCAAACCTTCGATCAA
GCGTTACTAGCACTTTATAATACCAATATGATTAGTTATACCGAGGCTTTACATCATGCTGACTCGCCAAATGATTTACG
CTTAATGATTAAGTTAAATCAAGGCCAAGCAGGCAGTGATAGTATGCTTGACGGGGTGACGATTGAAGCTTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.563

98.652

0.588
  pilU Pseudomonas stutzeri DSM 10701

57.923

98.652

0.571
  pilU Acinetobacter baylyi ADP1

58.453

94.07

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.733

92.722

0.396
  pilT Pseudomonas aeruginosa PAK

42.773

91.375

0.391
  pilT Pseudomonas stutzeri DSM 10701

41.298

91.375

0.377
  pilT Legionella pneumophila strain Lp02

41.641

88.679

0.369
  pilT Legionella pneumophila strain ERS1305867

41.641

88.679

0.369
  pilT Acinetobacter baumannii D1279779

40

91.644

0.367
  pilT Acinetobacter baumannii strain A118

40

91.644

0.367
  pilT Acinetobacter nosocomialis M2

40

91.644

0.367
  pilT Acinetobacter baylyi ADP1

39.881

90.566

0.361
  pilT Neisseria gonorrhoeae MS11

40

90.296

0.361
  pilT Vibrio cholerae strain A1552

43.506

83.019

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.506

83.019

0.361