NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	HWQ56_RS24615	Genome accession	NZ_CP056030
Coordinates	5512360..5512773 (-)	Length	137 a.a.
NCBI ID	WP_176571985.1	Uniprot ID	A0A7D5H2S5
Organism	Pseudomonas eucalypticola strain NP-1
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5507360..5517773

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HWQ56_RS24585 (HWQ56_24585)	-	5507737..5508000 (+)	264	WP_158152716.1	hypothetical protein	-
HWQ56_RS24590 (HWQ56_24590)	-	5508127..5508900 (-)	774	WP_176571982.1	EAL domain-containing protein	-
HWQ56_RS24595 (HWQ56_24595)	-	5509187..5509345 (-)	159	WP_176571983.1	hypothetical protein	-
HWQ56_RS24600 (HWQ56_24600)	-	5509335..5511092 (-)	1758	WP_176571984.1	anti-phage dCTP deaminase	-
HWQ56_RS24610 (HWQ56_24610)	-	5511879..5512307 (-)	429	WP_176572481.1	pilin	-
HWQ56_RS24615 (HWQ56_24615)	pilA	5512360..5512773 (-)	414	WP_176571985.1	pilin	Machinery gene
HWQ56_RS24620 (HWQ56_24620)	pilB	5513004..5514704 (+)	1701	WP_176571986.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HWQ56_RS24625 (HWQ56_24625)	pilC	5514708..5515931 (+)	1224	WP_176571987.1	type II secretion system F family protein	Machinery gene
HWQ56_RS24630 (HWQ56_24630)	pilD	5515934..5516803 (+)	870	WP_176571988.1	A24 family peptidase	Machinery gene
HWQ56_RS24635 (HWQ56_24635)	coaE	5516803..5517423 (+)	621	WP_158159036.1	dephospho-CoA kinase	-
HWQ56_RS24640 (HWQ56_24640)	yacG	5517423..5517626 (+)	204	WP_158159034.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 137 a.a. Molecular weight: 13746.86 Da Isoelectric Point: 8.5025

                                >NTDB_id=457963 HWQ56_RS24615 WP_176571985.1 5512360..5512773(-) (pilA) [Pseudomonas eucalypticola strain NP-1]

MKNQKGFTLIELMVVVAIIGILAAIAIPQYTRYQAKSKVAAAVAETSAMKVGVDDAVNSNTAPTLALAGGAASSANCSAI

AIAGSATANVTITCTITNAPSIVQGKNVVWTRTADTGVWACSSTVTDTTLLPKSCGG

Nucleotide

Download Length: 414 bp

                                >NTDB_id=457963 HWQ56_RS24615 WP_176571985.1 5512360..5512773(-) (pilA) [Pseudomonas eucalypticola strain NP-1]

ATGAAAAATCAGAAAGGTTTTACGTTAATCGAGCTGATGGTTGTAGTTGCAATCATCGGTATCCTTGCGGCAATCGCAAT

TCCGCAATACACGCGTTATCAGGCTAAGTCCAAAGTCGCTGCCGCAGTCGCTGAAACGTCTGCGATGAAAGTGGGTGTAG

ACGACGCAGTAAACTCCAATACGGCCCCGACCTTGGCTTTGGCGGGCGGTGCGGCATCTAGTGCGAACTGCTCTGCAATC

GCTATTGCAGGCTCTGCGACAGCGAACGTTACCATCACCTGCACAATCACCAACGCCCCTTCGATTGTTCAGGGTAAAAA

CGTAGTTTGGACTCGTACGGCAGATACTGGTGTCTGGGCCTGCAGCTCCACTGTAACGGATACCACGCTGCTGCCTAAAA

GCTGTGGTGGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-135)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A7D5H2S5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Acinetobacter baumannii strain A118	49.64	100	0.504
pilA	Pseudomonas aeruginosa PAK	42.953	100	0.467
comP	Acinetobacter baylyi ADP1	40.132	100	0.445
pilA/pilAI	Pseudomonas stutzeri DSM 10701	42.336	100	0.423
pilA/pilAII	Pseudomonas stutzeri DSM 10701	41.606	100	0.416
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	38.732	100	0.401
pilA	Vibrio cholerae strain A1552	38.732	100	0.401
pilA	Vibrio cholerae C6706	38.732	100	0.401
pilA	Vibrio parahaemolyticus RIMD 2210633	41.732	92.701	0.387
pilA/pilA1	Eikenella corrodens VA1	33.544	100	0.387
pilA	Haemophilus influenzae 86-028NP	36.879	100	0.38
pilA	Haemophilus influenzae Rd KW20	36.17	100	0.372
pilA2	Legionella pneumophila str. Paris	40.65	89.781	0.365
pilA2	Legionella pneumophila strain ERS1305867	40.65	89.781	0.365

Detailed information NTG0457963