Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HWQ56_RS24625 Genome accession   NZ_CP056030
Coordinates   5514708..5515931 (+) Length   407 a.a.
NCBI ID   WP_176571987.1    Uniprot ID   -
Organism   Pseudomonas eucalypticola strain NP-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5509708..5520931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ56_RS24610 (HWQ56_24610) - 5511879..5512307 (-) 429 WP_176572481.1 pilin -
  HWQ56_RS24615 (HWQ56_24615) pilA 5512360..5512773 (-) 414 WP_176571985.1 pilin Machinery gene
  HWQ56_RS24620 (HWQ56_24620) pilB 5513004..5514704 (+) 1701 WP_176571986.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HWQ56_RS24625 (HWQ56_24625) pilC 5514708..5515931 (+) 1224 WP_176571987.1 type II secretion system F family protein Machinery gene
  HWQ56_RS24630 (HWQ56_24630) pilD 5515934..5516803 (+) 870 WP_176571988.1 A24 family peptidase Machinery gene
  HWQ56_RS24635 (HWQ56_24635) coaE 5516803..5517423 (+) 621 WP_158159036.1 dephospho-CoA kinase -
  HWQ56_RS24640 (HWQ56_24640) yacG 5517423..5517626 (+) 204 WP_158159034.1 DNA gyrase inhibitor YacG -
  HWQ56_RS24645 (HWQ56_24645) - 5517667..5518344 (-) 678 WP_176571989.1 energy-coupling factor ABC transporter permease -
  HWQ56_RS24650 (HWQ56_24650) - 5518341..5518808 (-) 468 WP_158159030.1 FAD/FMN-containing dehydrogenase -
  HWQ56_RS24655 (HWQ56_24655) - 5518920..5519549 (+) 630 WP_158159028.1 DUF1780 domain-containing protein -
  HWQ56_RS24660 (HWQ56_24660) - 5519651..5519824 (+) 174 WP_158159026.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44310.23 Da        Isoelectric Point: 10.0394

>NTDB_id=457965 HWQ56_RS24625 WP_176571987.1 5514708..5515931(+) (pilC) [Pseudomonas eucalypticola strain NP-1]
MAAKARAPKIFTYNWEGTDRKGVRITGELTGVSIPLVKAQLRKQGINPGKIKKKSPSLFGRGKKIKPMDIALFTRQMATM
MRAGVPLLQSFDIIGEGFDNPTMRQLVGDLKQEVAAGNSFATALRRNPQYFDELYCNLVDAGEQAGALETLLDRVATYKE
KTEALKAKIRKAMTYPAAVVVVAFIVSAILLIKVVPQFQIVFQGFGAELPAFTLMVIGLSEIMQSYWWIVLAGAIGIVIA
FRQAMRKSERFRDALDRLLLKLPIVGGILYKSSVARYARTLATTFAAGVPLVEALDSVSGATGNVVFKNAVGKIKQDVST
GMQLNFSMRSTGVFPTMAVQMTAIGEESGALDEMLAKVASYYEDEVDNLVDSLTSLMEPLIMAFLGVIVGGLVIAMYLPI
FQLGNVV

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=457965 HWQ56_RS24625 WP_176571987.1 5514708..5515931(+) (pilC) [Pseudomonas eucalypticola strain NP-1]
ATGGCTGCGAAAGCACGAGCGCCCAAAATTTTCACTTACAATTGGGAAGGTACTGATCGCAAAGGCGTCAGGATAACGGG
CGAACTAACGGGTGTGAGCATACCCCTAGTGAAAGCTCAGTTACGCAAGCAAGGCATCAATCCAGGAAAAATCAAAAAAA
AATCGCCTTCTCTTTTTGGGCGAGGTAAAAAAATCAAACCCATGGATATTGCTCTATTCACCCGCCAGATGGCAACGATG
ATGAGAGCAGGTGTGCCGCTACTACAGTCTTTTGACATCATTGGCGAGGGCTTTGACAACCCGACGATGCGTCAATTAGT
AGGGGACTTAAAGCAGGAAGTCGCAGCCGGCAACAGCTTCGCAACCGCATTGAGGAGAAACCCTCAATATTTCGACGAGC
TCTACTGCAACTTAGTAGATGCCGGGGAGCAAGCAGGCGCGCTTGAGACACTGCTGGACCGGGTAGCTACCTATAAAGAG
AAGACGGAAGCCTTAAAAGCAAAAATACGCAAAGCGATGACGTATCCAGCGGCCGTAGTCGTCGTTGCTTTTATAGTTTC
AGCAATCCTATTAATCAAAGTGGTACCTCAATTCCAAATAGTATTTCAGGGATTTGGCGCAGAGCTTCCTGCTTTTACTT
TGATGGTAATAGGGCTTTCGGAAATAATGCAGAGTTATTGGTGGATAGTATTAGCTGGCGCCATCGGTATTGTCATAGCA
TTTAGGCAGGCCATGCGAAAAAGCGAGAGATTCCGGGATGCGCTGGATCGCCTGTTGCTAAAGCTGCCAATTGTCGGTGG
AATTTTATACAAGTCCTCGGTCGCCCGTTACGCTCGGACCTTAGCTACCACTTTTGCTGCAGGAGTTCCTTTAGTCGAGG
CACTAGACTCTGTCTCAGGCGCTACAGGTAATGTCGTTTTCAAAAACGCGGTAGGTAAAATCAAGCAAGACGTCTCCACC
GGCATGCAGCTTAACTTCTCGATGCGATCAACCGGCGTTTTCCCAACCATGGCAGTACAAATGACTGCCATCGGAGAGGA
GTCTGGTGCTTTGGACGAAATGCTTGCCAAGGTTGCCTCTTATTACGAGGACGAGGTAGATAACTTAGTAGACAGCTTAA
CCAGCCTGATGGAACCTCTTATCATGGCCTTCCTCGGCGTAATCGTAGGCGGGCTTGTAATCGCCATGTACCTCCCCATC
TTCCAACTCGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(73-196)

Type II secretion system (T2SS), protein F (T2SSF)

(278-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.641

100

0.776
  pilC Acinetobacter baylyi ADP1

60.88

100

0.612
  pilC Acinetobacter baumannii D1279779

59.169

100

0.595
  pilC Legionella pneumophila strain ERS1305867

52.109

99.017

0.516
  pilG Neisseria meningitidis 44/76-A

43.596

99.754

0.435
  pilG Neisseria gonorrhoeae MS11

43.35

99.754

0.432
  pilC Vibrio cholerae strain A1552

42.569

97.543

0.415
  pilC Vibrio campbellii strain DS40M4

41.481

99.509

0.413
  pilC Thermus thermophilus HB27

39

98.28

0.383