Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HWQ56_RS24620 Genome accession   NZ_CP056030
Coordinates   5513004..5514704 (+) Length   566 a.a.
NCBI ID   WP_176571986.1    Uniprot ID   A0A7D5HRD8
Organism   Pseudomonas eucalypticola strain NP-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5508004..5519704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ56_RS24590 (HWQ56_24590) - 5508127..5508900 (-) 774 WP_176571982.1 EAL domain-containing protein -
  HWQ56_RS24595 (HWQ56_24595) - 5509187..5509345 (-) 159 WP_176571983.1 hypothetical protein -
  HWQ56_RS24600 (HWQ56_24600) - 5509335..5511092 (-) 1758 WP_176571984.1 anti-phage dCTP deaminase -
  HWQ56_RS24610 (HWQ56_24610) - 5511879..5512307 (-) 429 WP_176572481.1 pilin -
  HWQ56_RS24615 (HWQ56_24615) pilA 5512360..5512773 (-) 414 WP_176571985.1 pilin Machinery gene
  HWQ56_RS24620 (HWQ56_24620) pilB 5513004..5514704 (+) 1701 WP_176571986.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HWQ56_RS24625 (HWQ56_24625) pilC 5514708..5515931 (+) 1224 WP_176571987.1 type II secretion system F family protein Machinery gene
  HWQ56_RS24630 (HWQ56_24630) pilD 5515934..5516803 (+) 870 WP_176571988.1 A24 family peptidase Machinery gene
  HWQ56_RS24635 (HWQ56_24635) coaE 5516803..5517423 (+) 621 WP_158159036.1 dephospho-CoA kinase -
  HWQ56_RS24640 (HWQ56_24640) yacG 5517423..5517626 (+) 204 WP_158159034.1 DNA gyrase inhibitor YacG -
  HWQ56_RS24645 (HWQ56_24645) - 5517667..5518344 (-) 678 WP_176571989.1 energy-coupling factor ABC transporter permease -
  HWQ56_RS24650 (HWQ56_24650) - 5518341..5518808 (-) 468 WP_158159030.1 FAD/FMN-containing dehydrogenase -
  HWQ56_RS24655 (HWQ56_24655) - 5518920..5519549 (+) 630 WP_158159028.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62622.54 Da        Isoelectric Point: 5.6979

>NTDB_id=457964 HWQ56_RS24620 WP_176571986.1 5513004..5514704(+) (pilB) [Pseudomonas eucalypticola strain NP-1]
MNDIALTGLAKHLVQADLLTDKSAQQAYQQAQRNRTPFVHFLVQSKLVKSREIAEIASEQFGVSLLDLAMLDKDSQPKGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPTNHQAITDIQFSTGLTTEAILVEDDKLVDAIEKFFDSGSTGLEEMADVDLDG
LDVQAVDTEKDEPIVGQDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKNYRVRLRTDGILREVARPPINLAGRIAARL
KVMASLDISERRKPQDGRIKMRISKSKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEPDQKELYLQALRQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLQNMGVAGFNIATSVSLIIAQRLARKLCPHCKRAAEIPLETQESEGFPA
HLIGTFKIYEPVGCDHCNNGYKGRQGIYEVARNTQALQRLIMEQGNSIEIAEQMRKDGFNDLRTSGLIKVMQGVTSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=457964 HWQ56_RS24620 WP_176571986.1 5513004..5514704(+) (pilB) [Pseudomonas eucalypticola strain NP-1]
ATGAACGATATTGCCCTCACCGGCTTGGCCAAGCACTTGGTCCAGGCCGATTTGCTAACCGACAAGAGCGCGCAGCAGGC
GTATCAGCAAGCGCAGCGGAATCGTACCCCTTTCGTACATTTCTTGGTACAGAGCAAGCTAGTAAAAAGTCGTGAAATTG
CTGAGATAGCGTCTGAGCAATTTGGTGTCTCGCTTCTCGATCTAGCGATGCTGGATAAGGATAGCCAGCCTAAGGGGTTA
GTAAGCGAAAAACTAATCAGGCAACACCACGCACTTCCACTTTGGCGTCGTGGTAACAAGCTATTTGTTGGCGTATCGGA
TCCAACCAACCATCAAGCAATTACTGATATCCAGTTCAGCACTGGGTTGACCACCGAGGCCATCCTTGTCGAAGACGACA
AGCTCGTAGATGCGATTGAGAAATTCTTCGACTCCGGCAGCACTGGGCTTGAAGAAATGGCGGATGTCGACCTCGATGGT
CTTGATGTGCAGGCGGTTGATACTGAAAAAGATGAACCGATAGTAGGTCAGGACGCGGACGACGCTCCCGTGGTCCGTTT
TGTAAACAAAATGTTATTGGACGCCATTCGCGGAGGGTCATCAGACCTTCACTTTGAACCCTATGAAAAGAATTACCGCG
TACGATTGCGGACCGACGGCATATTACGCGAAGTTGCGCGCCCCCCCATCAATCTTGCAGGGCGCATTGCCGCACGCTTA
AAGGTCATGGCAAGCCTCGACATATCCGAAAGGCGAAAACCTCAGGATGGTCGTATAAAGATGCGCATATCGAAGAGCAA
AGCAATTGATTTTCGCGTCAACACTCTCCCAACACTTTGGGGTGAGAAAATTGTAATGCGAATTCTGGATCCGTCTAGCG
CTCAAATGGGTATTGATGCACTTGGGTACGAACCGGACCAGAAAGAACTTTACCTTCAGGCGCTCCGCCAGCCGCAAGGA
ATGATACTCGTTACTGGCCCCACAGGCTCTGGCAAAACCGTATCACTATACACTGGCCTTAATATCTTAAACACAGTGGA
TATCAACATTTCTACAGCTGAGGACCCGGTTGAAATCAACATGGAAGGCATTAACCAAGTTAACGTCAACCCTAAACAAG
GGCTGGACTTTGCCCAAGCACTGAGATCCTTTCTACGCCAGGATCCTGACGTCATCATGGTAGGTGAAATCCGCGATCTT
GAGACAGCAGAAATTGCCATTAAGGCCGCTCAAACGGGCCACCTTGTGCTATCTACACTACACACTAATAGTGCAGCAGA
GACGCTGACACGCTTGCAAAACATGGGCGTTGCTGGCTTCAACATTGCCACCTCTGTCAGCTTGATTATTGCACAAAGGT
TGGCACGCAAGCTCTGCCCTCATTGTAAACGAGCTGCGGAAATCCCTTTGGAAACACAGGAAAGTGAAGGCTTCCCTGCG
CACCTTATCGGAACTTTTAAAATCTATGAACCTGTAGGTTGCGACCACTGCAACAACGGGTACAAAGGTCGACAGGGCAT
TTATGAGGTAGCGCGAAATACCCAGGCGCTTCAGCGCCTTATCATGGAGCAAGGAAACTCCATCGAGATCGCGGAGCAAA
TGCGCAAAGACGGGTTCAATGATCTTCGCACCTCTGGTTTGATAAAGGTCATGCAGGGCGTGACTAGCCTTGAAGAAGTT
AACCGCGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D5HRD8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.377

100

0.585
  pilB Acinetobacter baylyi ADP1

58.082

99.47

0.578
  pilB Legionella pneumophila strain ERS1305867

55.221

99.823

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

52.297

100

0.523
  pilB Vibrio cholerae strain A1552

52.305

99.647

0.521
  pilB Vibrio campbellii strain DS40M4

51.413

100

0.514
  pilF Neisseria gonorrhoeae MS11

50.355

99.647

0.502
  pilF Thermus thermophilus HB27

37.455

98.587

0.369