Detailed information NTG0457966

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HWQ56_RS24630
• Genome accession: NZ_CP056030
• Coordinates: 5515934..5516803 (+)
• Length: 289 a.a.
• NCBI ID: WP_176571988.1
• Uniprot ID: A0A7D5HG49
• Organism: Pseudomonas eucalypticola strain NP-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5510934..5521803

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HWQ56_RS24610 (HWQ56_24610)	-	5511879..5512307 (-)	429	WP_176572481.1	pilin	-
HWQ56_RS24615 (HWQ56_24615)	pilA	5512360..5512773 (-)	414	WP_176571985.1	pilin	Machinery gene
HWQ56_RS24620 (HWQ56_24620)	pilB	5513004..5514704 (+)	1701	WP_176571986.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HWQ56_RS24625 (HWQ56_24625)	pilC	5514708..5515931 (+)	1224	WP_176571987.1	type II secretion system F family protein	Machinery gene
HWQ56_RS24630 (HWQ56_24630)	pilD	5515934..5516803 (+)	870	WP_176571988.1	A24 family peptidase	Machinery gene
HWQ56_RS24635 (HWQ56_24635)	coaE	5516803..5517423 (+)	621	WP_158159036.1	dephospho-CoA kinase	-
HWQ56_RS24640 (HWQ56_24640)	yacG	5517423..5517626 (+)	204	WP_158159034.1	DNA gyrase inhibitor YacG	-
HWQ56_RS24645 (HWQ56_24645)	-	5517667..5518344 (-)	678	WP_176571989.1	energy-coupling factor ABC transporter permease	-
HWQ56_RS24650 (HWQ56_24650)	-	5518341..5518808 (-)	468	WP_158159030.1	FAD/FMN-containing dehydrogenase	-
HWQ56_RS24655 (HWQ56_24655)	-	5518920..5519549 (+)	630	WP_158159028.1	DUF1780 domain-containing protein	-
HWQ56_RS24660 (HWQ56_24660)	-	5519651..5519824 (+)	174	WP_158159026.1	DUF3094 domain-containing protein	-
HWQ56_RS24665 (HWQ56_24665)	-	5519912..5521207 (+)	1296	WP_176571990.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31406.54 Da
• Isoelectric Point: 8.3523

>NTDB_id=457966 HWQ56_RS24630 WP_176571988.1 5515934..5516803(+) (pilD) [Pseudomonas eucalypticola strain NP-1]
MPLSDYLATTPIAFTLCALILGLVVGSFLNVIIHRLPKMLERDWKAQARDILGLPAEPEGPRYNLMLPASTCPHCAHKIR
AWENIPVVSYLALKGKCNQCHAPISKRYPLVELASGVLSAFIAWHFGFGWAAGAMMLLTWGLLAMSLIDADHQLLPDALV
LPLLWLGLIVNAFGLFATLPDALWGAVGGYLALWVVFWLFKLVTGKEGMGHGDFKLLAMLGAWGGWQVLPLTILLSSLVG
AILGVILLRLRNAAHSTPIPFGPYLAIAGWIALLWGGQITASYMQFAGF

Nucleotide

• Length: 870 bp

>NTDB_id=457966 HWQ56_RS24630 WP_176571988.1 5515934..5516803(+) (pilD) [Pseudomonas eucalypticola strain NP-1]
ATGCCCCTATCCGACTACCTGGCCACCACCCCCATAGCCTTCACCCTCTGCGCCCTGATCCTCGGCCTGGTCGTAGGCAG
CTTCCTGAACGTCATCATCCACCGCCTCCCCAAGATGCTGGAACGCGACTGGAAAGCCCAAGCCCGGGACATTCTCGGCC
TCCCCGCCGAGCCCGAGGGGCCGCGCTACAACCTGATGCTCCCCGCCTCCACCTGTCCCCACTGCGCCCATAAGATCCGC
GCCTGGGAGAACATTCCCGTGGTCAGCTACCTGGCGCTGAAAGGCAAGTGCAACCAATGCCATGCCCCCATCAGCAAACG
CTACCCGCTGGTAGAGCTGGCCAGCGGCGTGCTCAGCGCGTTCATTGCCTGGCATTTCGGTTTCGGCTGGGCCGCTGGGG
CGATGATGTTGCTGACCTGGGGGCTGTTGGCCATGAGCCTGATCGACGCCGACCACCAGTTGTTGCCCGATGCGCTGGTG
TTGCCGCTGTTGTGGCTGGGCCTGATCGTCAACGCGTTCGGGTTGTTCGCGACTTTGCCCGATGCGCTGTGGGGCGCGGT
GGGCGGGTACCTGGCGCTGTGGGTCGTGTTCTGGCTGTTCAAGTTGGTGACCGGCAAGGAAGGCATGGGCCATGGCGACT
TCAAGTTGTTGGCCATGCTCGGCGCCTGGGGTGGGTGGCAGGTGTTGCCGCTGACCATTCTGCTGTCGTCGCTGGTGGGT
GCCATTCTGGGGGTGATTCTGTTGCGCTTGCGCAACGCGGCGCATAGCACGCCGATCCCTTTTGGGCCTTACTTGGCCAT
CGCGGGCTGGATCGCATTGCTCTGGGGTGGTCAAATAACCGCTTCTTACATGCAGTTCGCTGGTTTCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

3D structure

• AlphaFold DB: A0A7D5HG49

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.047	96.54	0.512
pilD	Vibrio campbellii strain DS40M4	52.174	95.502	0.498
pilD	Acinetobacter baumannii D1279779	49.11	97.232	0.478
pilD	Acinetobacter nosocomialis M2	48.043	97.232	0.467
pilD	Neisseria gonorrhoeae MS11	49.63	93.426	0.464