Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THTHE16_RS00090 Genome accession   NC_017272
Coordinates   17780..18865 (-) Length   361 a.a.
NCBI ID   WP_014509695.1    Uniprot ID   F6DH58
Organism   Thermus thermophilus SG0.5JP17-16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 12780..23865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THTHE16_RS00075 (Ththe16_0012) - 14724..15356 (-) 633 WP_008634004.1 histidine phosphatase family protein -
  THTHE16_RS00080 (Ththe16_0013) purM 15353..16354 (-) 1002 WP_014509693.1 phosphoribosylformylglycinamidine cyclo-ligase -
  THTHE16_RS00085 (Ththe16_0014) gatB 16360..17769 (-) 1410 WP_014509694.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  THTHE16_RS00090 (Ththe16_0015) pilT 17780..18865 (-) 1086 WP_014509695.1 type IV pilus twitching motility protein PilT Machinery gene
  THTHE16_RS00095 (Ththe16_0016) pilF 18878..21547 (-) 2670 WP_014509696.1 type IV pilus assembly ATPase PilB Machinery gene
  THTHE16_RS00100 (Ththe16_0017) - 21544..22029 (-) 486 WP_008634008.1 YqeG family HAD IIIA-type phosphatase -
  THTHE16_RS00105 (Ththe16_0018) pgeF 22062..22787 (-) 726 WP_014509697.1 peptidoglycan editing factor PgeF -
  THTHE16_RS00110 (Ththe16_0019) surE 22784..23539 (-) 756 WP_014509698.1 5'/3'-nucleotidase SurE -
  THTHE16_RS00115 (Ththe16_0020) - 23596..23802 (-) 207 WP_008634012.1 cold-shock protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 39962.29 Da        Isoelectric Point: 7.5124

>NTDB_id=45766 THTHE16_RS00090 WP_014509695.1 17780..18865(-) (pilT) [Thermus thermophilus SG0.5JP17-16]
MAKAPDVVDLLTLAVERGASDLVITVGLPPMIKVDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRFRVNVFLQRGSVGGVLRVVPSAVKSFEELGLPKNIAEIAMSPRGLVLVTGPTGSGKSTTLAAMIDYINERKACHIV
TIEDPIEFFHKHKRAIVNQREIGSDTKSFHRALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKTHQLRSVIQTSGQYGMIT
MDACLADLYKRKLITYEMGLARAVDPKEFMRLAGAPEGARR

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=45766 THTHE16_RS00090 WP_014509695.1 17780..18865(-) (pilT) [Thermus thermophilus SG0.5JP17-16]
ATGGCCAAAGCCCCAGACGTGGTGGACCTTTTGACGCTCGCGGTGGAACGGGGGGCGAGCGACCTCGTGATCACCGTGGG
CCTGCCCCCCATGATCAAGGTGGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAACAGCGGATCTTTGAGGAGGAGAAAGAACTAGACTTCTCCTTCAGCCTCCCG
GGCAAGGGGCGCTTCCGGGTGAACGTCTTCCTGCAACGGGGAAGCGTGGGCGGGGTCTTACGGGTGGTGCCGAGCGCTGT
GAAGAGCTTTGAGGAGCTCGGCCTCCCCAAGAACATCGCCGAGATCGCCATGAGCCCAAGGGGCCTCGTCCTGGTCACGG
GGCCCACAGGGTCTGGCAAGAGCACCACCCTGGCCGCCATGATCGACTACATCAACGAACGCAAAGCCTGCCACATCGTC
ACCATCGAGGACCCCATAGAGTTTTTCCACAAGCACAAGCGGGCCATCGTGAACCAACGGGAGATCGGCTCCGACACCAA
GAGCTTCCACCGGGCGCTCCGCTCGGTCCTCCGCCAGGCCCCGGACGTGATCCTCGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACGGGGCATCTGGTCATGGGCACCCTCCACACCAACTCCGCCCCGGAAACC
ATAGACCGCATCGTGGACGTCTTCCCGGAAAACCAGCAGGAACAGGTCCGGGTGCAGCTCTCCAACAACCTGGTGGCCGT
CCTCACGCAACAGCTCCTGCCCAAGGCCTTTGGCGGCGGGCGGGTCCTGGCCTACGAGCTCATGATCGCCACCCCAGCCG
TGAGGGCCCTCATCCGGGAGGGGAAGACCCACCAGTTAAGGAGCGTGATCCAGACGAGCGGCCAGTACGGGATGATCACC
ATGGACGCCTGCCTCGCCGACCTGTACAAGCGCAAGCTCATCACCTACGAAATGGGGCTTGCCAGGGCCGTGGACCCCAA
GGAGTTCATGAGGCTCGCGGGCGCGCCCGAGGGCGCCAGGCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6DH58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.705

97.784

0.662
  pilT Pseudomonas aeruginosa PAK

54.651

95.291

0.521
  pilT Pseudomonas stutzeri DSM 10701

54.36

95.291

0.518
  pilT Acinetobacter baylyi ADP1

54.36

95.291

0.518
  pilT Acinetobacter nosocomialis M2

53.333

95.568

0.51
  pilT Acinetobacter baumannii D1279779

53.333

95.568

0.51
  pilT Acinetobacter baumannii strain A118

53.333

95.568

0.51
  pilT Neisseria meningitidis 8013

52.959

93.629

0.496
  pilT Neisseria gonorrhoeae MS11

52.226

93.352

0.488
  pilT Legionella pneumophila strain ERS1305867

51.163

95.291

0.488
  pilT Legionella pneumophila strain Lp02

51.163

95.291

0.488
  pilT Vibrio cholerae strain A1552

51.672

91.136

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.672

91.136

0.471
  pilU Pseudomonas stutzeri DSM 10701

47.813

95.014

0.454
  pilU Vibrio cholerae strain A1552

45.21

92.521

0.418
  pilU Acinetobacter baylyi ADP1

42.609

95.568

0.407

Multiple sequence alignment