Detailed information NTG0045767

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: THTHE16_RS00095
• Genome accession: NC_017272
• Coordinates: 18878..21547 (-)
• Length: 889 a.a.
• NCBI ID: WP_014509696.1
• Uniprot ID: F6DH59
• Organism: Thermus thermophilus SG0.5JP17-16
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 13878..26547

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
THTHE16_RS00075 (Ththe16_0012)	-	14724..15356 (-)	633	WP_008634004.1	histidine phosphatase family protein	-
THTHE16_RS00080 (Ththe16_0013)	purM	15353..16354 (-)	1002	WP_014509693.1	phosphoribosylformylglycinamidine cyclo-ligase	-
THTHE16_RS00085 (Ththe16_0014)	gatB	16360..17769 (-)	1410	WP_014509694.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
THTHE16_RS00090 (Ththe16_0015)	pilT	17780..18865 (-)	1086	WP_014509695.1	type IV pilus twitching motility protein PilT	Machinery gene
THTHE16_RS00095 (Ththe16_0016)	pilF	18878..21547 (-)	2670	WP_014509696.1	type IV pilus assembly ATPase PilB	Machinery gene
THTHE16_RS00100 (Ththe16_0017)	-	21544..22029 (-)	486	WP_008634008.1	YqeG family HAD IIIA-type phosphatase	-
THTHE16_RS00105 (Ththe16_0018)	pgeF	22062..22787 (-)	726	WP_014509697.1	peptidoglycan editing factor PgeF	-
THTHE16_RS00110 (Ththe16_0019)	surE	22784..23539 (-)	756	WP_014509698.1	5'/3'-nucleotidase SurE	-
THTHE16_RS00115 (Ththe16_0020)	-	23596..23802 (-)	207	WP_008634012.1	cold-shock protein	-
THTHE16_RS00120 (Ththe16_0021)	-	23958..25247 (+)	1290	WP_014509699.1	ABC transporter substrate-binding protein	-
THTHE16_RS00125 (Ththe16_0022)	-	25298..26173 (+)	876	WP_014509700.1	carbohydrate ABC transporter permease	-

Sequence

Protein

• Length: 889 a.a.
• Molecular weight: 98034.23 Da
• Isoelectric Point: 5.3937

>NTDB_id=45767 THTHE16_RS00095 WP_014509696.1 18878..21547(-) (pilF) [Thermus thermophilus SG0.5JP17-16]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSLEEVEDLTGLVVEPYQTTKSDFLYALAKHYPELGLPVPPPPSG
EGQKDLMLGEFLLKKGLISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGMEFLESAEGIVPDPSAAALL
LRSDALRYGAVPIGFQNGKVEVVLSDPRHKETVAQLLKRPARFYLALPQAWEELFGRAYPEKNRLGEVLVQEGKLSREAL
KEALEVQKGLPRAKPLGEILVELGLARPEDVEEALKKQRQGGGRLEDTLIQSGKLKPEALAQAVATQLGYPYVDPEEDPP
DPGASLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLNHEVAPAVATEAAITKLIERFYSKAEL
SEIAKEFAQKQVVEEAPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVI
SVVKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGL
SEAEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTL
EEVLARTIE

Nucleotide

• Length: 2670 bp

>NTDB_id=45767 THTHE16_RS00095 WP_014509696.1 18878..21547(-) (pilF) [Thermus thermophilus SG0.5JP17-16]
ATGAGCGTTCTTACCATAGGGGACAAAAGGCTTGGGGCGGCCCTGTTGGACGCCGGCCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGTCAAG
GCCCTCCTTCCCGCGGAAAAGGCCAAGGAGCTCAAGGCCATCCCCTTTGCCTTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCCTCGAGGAGGTGGAGGACCTCACGGGGCTCGTGGTGGAGCCGTACCAAA
CCACCAAAAGCGACTTCCTTTACGCCCTGGCCAAGCACTACCCGGAGCTTGGCCTTCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAAAAGGACCTCATGCTCGGGGAGTTCCTCCTGAAAAAGGGGCTGATCTCCCGCGAGGCCCTGGAGGAGGCCCT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGAAAAGGGCTTCCCGAGGAGGCCCTTTACCGGG
CTTTGGCGGAGCAGAAGGGAATGGAGTTTCTGGAAAGCGCCGAGGGAATCGTCCCCGACCCCTCCGCCGCCGCCCTCCTC
CTCCGCTCCGACGCCCTGCGCTACGGCGCCGTGCCCATCGGCTTCCAAAACGGGAAGGTGGAGGTGGTCCTCTCCGACCC
ACGGCACAAGGAGACGGTGGCCCAGCTCCTCAAGCGGCCCGCCCGCTTCTACCTCGCCCTTCCCCAGGCCTGGGAGGAGC
TTTTCGGCCGGGCCTACCCCGAGAAGAACCGCCTGGGGGAGGTCCTGGTTCAGGAGGGCAAGCTCTCCAGGGAGGCGCTA
AAGGAAGCCCTGGAGGTGCAGAAAGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCG
CCCCGAGGACGTGGAAGAAGCCCTCAAGAAGCAGCGCCAAGGGGGGGGAAGGCTGGAGGACACCCTGATCCAGTCGGGAA
AGCTCAAACCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACCCCTACGTTGACCCCGAGGAGGATCCCCCC
GATCCCGGCGCCTCCCTCCTCCTCCCGGAGGACCTCTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGGAACCG
CCTCGTCCTCCTGATGAAAGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGCGGAAGGGCCTGA
ACCACGAGGTGGCCCCGGCGGTGGCCACCGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACAGCAAGGCCGAGCTC
TCGGAGATCGCCAAGGAGTTCGCCCAAAAACAGGTGGTGGAAGAGGCTCCAAGTCCCCTGGAGCTGGACGAGAGCGCCGC
CCAGAAGTTCGTGAAGCAGGTGATCCGGGAAGCCTTCCTCCAGGACGCCTCCGACATCCACATTGAGCCCAGGCAGAACG
ACGTCCAGGTGCGCCTAAGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCCAAGGGGGCGCTGAACGCGGTGATC
TCCGTGGTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAGCGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAAGG
GGCCATAGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGG
CCTCGGACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCC
TACGGCATCTTCCTCATCACCGGACCCACGGGGTCGGGCAAGAGCTTTACCACCTTCTCCATCCTCAAGCGCATCGCCAC
CCCCGACAAGAACACCCAGACCATTGAGGACCCCGTGGAGTACGAGATTCCCGGGATCAACCAGACCCAGGTGAACCCGC
AGGCGGGCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGG
GACTCCGAGACGGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGC
CGCCCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAACCCTTTAACATCTCCGCCGCCCTCATCGGCGTCCTCTCCC
AGCGCCTGGTGCGCAGGGTGTGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTCAGGCGCCTCGGGCTT
TCCGAGGCGGAGATCCAAGGGGCCAGGCTCTACAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGGTACAAGGGCCG
CTACGCCATTCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCA
AGGAGATCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (513-779)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-149)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

3D structure

• AlphaFold DB: F6DH59

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	97.3	100	0.973
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	55.506	100	0.556