Detailed information NTG0456600

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: HUZ50_RS04095
• Genome accession: NZ_CP055167
• Coordinates: 844565..845545 (+)
• Length: 326 a.a.
• NCBI ID: WP_239612956.1
• Uniprot ID: -
• Organism: Shigella flexneri strain STEFF_24
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	845821..846801	844565..845545	flank	276

Gene organization within MGE regions

Location: 844565..846801

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUZ50_RS04095 (HUZ50_04040)	pilT	844565..845545 (+)	981	WP_239612956.1	type IV pilus twitching motility protein PilT	Machinery gene
HUZ50_RS04100 (HUZ50_04045)	-	845821..846801 (+)	981	WP_000399648.1	IS110-like element IS621 family transposase	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35893.04 Da
• Isoelectric Point: 5.5860

>NTDB_id=456600 HUZ50_RS04095 WP_239612956.1 844565..845545(+) (pilT) [Shigella flexneri strain STEFF_24]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLSSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=456600 HUZ50_RS04095 WP_239612956.1 844565..845545(+) (pilT) [Shigella flexneri strain STEFF_24]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTATCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Acinetobacter baylyi ADP1	46.177	100	0.463
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.566	100	0.457
pilT	Legionella pneumophila strain ERS1305867	44.648	100	0.448
pilT	Legionella pneumophila strain Lp02	44.648	100	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	37.037	99.387	0.368
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365