Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HUW35_RS05655 Genome accession   NZ_CP055157
Coordinates   1338667..1339701 (+) Length   344 a.a.
NCBI ID   WP_181254644.1    Uniprot ID   A0A7H8PW83
Organism   Microbulbifer sp. YPW1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1333667..1344701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW35_RS05635 (HUW35_05640) - 1334069..1336045 (-) 1977 WP_181254641.1 dynamin family protein -
  HUW35_RS05640 (HUW35_05645) - 1336244..1336849 (-) 606 WP_078082709.1 YggT family protein -
  HUW35_RS05645 (HUW35_05650) proC 1337014..1337841 (-) 828 WP_181254642.1 pyrroline-5-carboxylate reductase -
  HUW35_RS05650 (HUW35_05655) - 1337872..1338567 (-) 696 WP_181254643.1 YggS family pyridoxal phosphate-dependent enzyme -
  HUW35_RS05655 (HUW35_05660) pilT 1338667..1339701 (+) 1035 WP_181254644.1 type IV pilus twitching motility protein PilT Machinery gene
  HUW35_RS05660 (HUW35_05665) pilU 1339941..1341089 (+) 1149 WP_181254645.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HUW35_RS05665 (HUW35_05670) - 1341188..1342879 (+) 1692 WP_181254646.1 amidohydrolase -
  HUW35_RS05670 (HUW35_05675) - 1342894..1343658 (-) 765 WP_181254647.1 LytTR family DNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38400.99 Da        Isoelectric Point: 6.4349

>NTDB_id=456439 HUW35_RS05655 WP_181254644.1 1338667..1339701(+) (pilT) [Microbulbifer sp. YPW1]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGQVFKQISDTPRGLVLVTGPTGSGKSTSLAAMIDYINDNKYEHILTVED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVISQTLLKRNGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGASVGMQTMDQCL
QDLVEKRIISRETAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=456439 HUW35_RS05655 WP_181254644.1 1338667..1339701(+) (pilT) [Microbulbifer sp. YPW1]
ATGGACATTACAGAACTCCTCGCCTTCAGCGCCAAGCAGAACGCCTCGGACCTGCACCTCTCCGCCGGCCTGCCACCCAT
GATCCGGGTCGACGGCGACGTGCGCCGCATCAACCTGCCGCCCATGGAGCACAAGCAGGTACACGGTCTGATCTACGAGA
TCATGAACGACAAGCAGCGCAAGGACTACGAAGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTCCCCGGCGTGGCCCGC
TTCCGTGTGAACGCGTTCAACCACAACCGCGGCGCCGGCGCCGTATTCCGGACCATTCCCTCCAAGGTACTCACCATGGA
AGACCTCGGAATGGGCCAGGTGTTCAAACAGATCTCCGACACCCCCCGCGGCCTGGTACTCGTCACCGGTCCTACCGGCT
CCGGTAAGTCCACCTCGCTCGCGGCGATGATCGACTACATCAACGACAACAAGTACGAGCACATCCTCACCGTCGAAGAC
CCGATCGAATTCGTACACGAATCCAAGAAGTGCCTGGTCAACCAGCGGGAAGTCCACCGCGACACCCACGGCTTCTCCGA
AGCCCTGCGCTCCGCACTGCGTGAAGACCCCGACATCATCCTCGTGGGTGAGATGCGAGACCTGGAAACCATCCGCCTGG
CACTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACAACCTCCGCCGCCAAAACCATCGACCGTGTG
GTAGACGTATTCCCGGCGGAGGAAAAAGCCATGGTGCGCTCGATGCTCTCAGAATCCCTGCAGGCAGTAATCTCCCAAAC
CCTGCTCAAACGCAACGGCGGCGGCCGGGTTGCCGCCCATGAAATCATGCGCGGCACTCCCGCCATCCGGAACCTGATTC
GCGAAGACAAGATCGCGCAGATGTACTCCGCCATCCAGACCGGCGCCAGCGTCGGCATGCAGACCATGGACCAGTGTCTG
CAGGATCTGGTGGAGAAACGCATCATCAGCCGCGAAACTGCGCGCGAGAAAGCGAAAATGCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8PW83

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

89.535

100

0.895
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

80.814

100

0.808
  pilT Legionella pneumophila strain Lp02

76.331

98.256

0.75
  pilT Legionella pneumophila strain ERS1305867

76.331

98.256

0.75
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.647

98.837

0.669
  pilT Vibrio cholerae strain A1552

67.647

98.837

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

40.413

98.547

0.398
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392