Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HUW35_RS05660 Genome accession   NZ_CP055157
Coordinates   1339941..1341089 (+) Length   382 a.a.
NCBI ID   WP_181254645.1    Uniprot ID   -
Organism   Microbulbifer sp. YPW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1334941..1346089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW35_RS05640 (HUW35_05645) - 1336244..1336849 (-) 606 WP_078082709.1 YggT family protein -
  HUW35_RS05645 (HUW35_05650) proC 1337014..1337841 (-) 828 WP_181254642.1 pyrroline-5-carboxylate reductase -
  HUW35_RS05650 (HUW35_05655) - 1337872..1338567 (-) 696 WP_181254643.1 YggS family pyridoxal phosphate-dependent enzyme -
  HUW35_RS05655 (HUW35_05660) pilT 1338667..1339701 (+) 1035 WP_181254644.1 type IV pilus twitching motility protein PilT Machinery gene
  HUW35_RS05660 (HUW35_05665) pilU 1339941..1341089 (+) 1149 WP_181254645.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HUW35_RS05665 (HUW35_05670) - 1341188..1342879 (+) 1692 WP_181254646.1 amidohydrolase -
  HUW35_RS05670 (HUW35_05675) - 1342894..1343658 (-) 765 WP_181254647.1 LytTR family DNA-binding domain-containing protein -
  HUW35_RS05675 (HUW35_05680) - 1343655..1344857 (-) 1203 WP_181254648.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42475.70 Da        Isoelectric Point: 6.0944

>NTDB_id=456440 HUW35_RS05660 WP_181254645.1 1339941..1341089(+) (pilU) [Microbulbifer sp. YPW1]
MEIERLLQLVTEKGASDLFITAGVPPSIKLHGKIVPVSSSPLSPEKAREMVVGIMNDRQKKEFAESKELNFAISLRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDGLGLPEQLKELAMTKRGLIIFVGATGTGKSTSLASMIGHRNENSKGHIISIED
PIEFIHQHRGCIVTQREVGLDTDSFETALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHQQLWMDLSLNLQAMVAQQLVPTPDGDGRRACLEIMINTPLMSDLIRKGEVHKMKELMKRSVEQGMQTFDQA
LYELYDRGEITYEDALAHADSANDLRLMIKLKSESDAEYLNSAADELSLASDSDSGGGPQIY

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=456440 HUW35_RS05660 WP_181254645.1 1339941..1341089(+) (pilU) [Microbulbifer sp. YPW1]
ATGGAAATCGAACGACTCCTGCAACTGGTCACCGAAAAAGGCGCCTCCGACCTGTTTATCACCGCCGGTGTGCCGCCGTC
CATCAAACTGCACGGCAAAATCGTCCCCGTAAGCAGCTCCCCGCTAAGCCCGGAAAAAGCGCGCGAAATGGTCGTCGGCA
TCATGAACGATCGCCAGAAAAAAGAGTTCGCCGAAAGCAAAGAACTCAACTTCGCCATCTCCCTGCGCAACGTCGGCCGC
TTCCGGGTGTCCGCCTTCTTCCAGCGCAACCTCGTCGGCATGGTACTGCGTCGGATCGAAACCAAAATCCCCACCATCGA
CGGACTGGGGCTGCCAGAACAGCTCAAAGAACTGGCCATGACCAAGCGCGGCCTGATCATCTTCGTGGGCGCAACGGGTA
CCGGTAAATCCACCTCGCTCGCCTCCATGATCGGCCACCGCAATGAAAACTCGAAGGGTCACATCATCTCCATCGAAGAC
CCCATCGAATTCATTCACCAGCACCGCGGATGTATCGTCACCCAGCGCGAAGTCGGCCTCGACACCGACTCCTTCGAGAC
CGCCCTGAAAAACACCCTGCGACAGGCCCCGGACGTCATCCTCATTGGTGAGGTGCGCTCCCGCGAAACCATGGACCACG
CCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGTGTACCCTGCACGCCAACAACGCCAACCAGGCACTGGACCGGATC
ATCCACTTCTTCCCCGCCGACCGGCACCAGCAGCTGTGGATGGATTTGTCGCTCAACCTGCAGGCCATGGTTGCCCAGCA
GCTAGTACCGACACCGGACGGCGATGGTCGCCGCGCCTGTCTCGAAATCATGATCAACACCCCGCTCATGTCCGACCTCA
TCCGCAAGGGCGAGGTACACAAAATGAAGGAATTGATGAAGCGCTCCGTCGAGCAGGGTATGCAGACCTTCGACCAGGCC
CTGTACGAACTCTACGACCGCGGCGAGATCACCTACGAAGACGCCCTCGCCCACGCCGACTCCGCCAACGACCTGCGCCT
GATGATCAAGCTCAAGTCCGAGTCCGACGCCGAGTACCTCAACAGCGCCGCCGACGAACTCAGCCTCGCCAGCGATAGCG
ACAGCGGCGGCGGCCCGCAGATCTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.862

98.691

0.749
  pilU Acinetobacter baylyi ADP1

67.227

93.455

0.628
  pilU Vibrio cholerae strain A1552

55.337

93.194

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.133

88.743

0.401
  pilT Legionella pneumophila strain Lp02

42.985

87.696

0.377
  pilT Legionella pneumophila strain ERS1305867

42.985

87.696

0.377
  pilT Pseudomonas aeruginosa PAK

41.493

87.696

0.364
  pilT Acinetobacter nosocomialis M2

41.493

87.696

0.364
  pilT Acinetobacter baumannii D1279779

41.493

87.696

0.364
  pilT Acinetobacter baumannii strain A118

41.493

87.696

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

87.435

0.361
  pilT Vibrio cholerae strain A1552

41.317

87.435

0.361