Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HUK68_RS01320 Genome accession   NZ_CP054840
Coordinates   273187..274230 (-) Length   347 a.a.
NCBI ID   WP_175502569.1    Uniprot ID   A0A6N1WWN7
Organism   Comamonas antarctica strain 16-35-5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 268187..279230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK68_RS01290 (HUK68_01290) - 268490..268900 (+) 411 WP_175502563.1 YraN family protein -
  HUK68_RS01295 (HUK68_01295) - 268950..269558 (+) 609 WP_175502564.1 SIS domain-containing protein -
  HUK68_RS01300 (HUK68_01300) - 269555..270217 (+) 663 WP_175502565.1 BON domain-containing protein -
  HUK68_RS01305 (HUK68_01305) - 270346..271242 (-) 897 WP_175502566.1 NAD(P)-dependent oxidoreductase -
  HUK68_RS01310 (HUK68_01310) pilU 271293..272429 (-) 1137 WP_175502567.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HUK68_RS01315 (HUK68_01315) - 272472..273110 (-) 639 WP_175502568.1 cyclic nucleotide-binding domain-containing protein -
  HUK68_RS01320 (HUK68_01320) pilT 273187..274230 (-) 1044 WP_175502569.1 type IV pilus twitching motility protein PilT Machinery gene
  HUK68_RS01325 (HUK68_01325) - 274269..274997 (+) 729 WP_175502570.1 YggS family pyridoxal phosphate-dependent enzyme -
  HUK68_RS01330 (HUK68_01330) - 275133..276218 (-) 1086 WP_175502571.1 glycosyl hydrolase family 79 C-terminal domain-containing protein -
  HUK68_RS01335 (HUK68_01335) - 276509..277654 (+) 1146 WP_175502572.1 aminotransferase class V-fold PLP-dependent enzyme -
  HUK68_RS01340 (HUK68_01340) - 277675..278100 (+) 426 WP_175502573.1 FKBP-type peptidyl-prolyl cis-trans isomerase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38328.15 Da        Isoelectric Point: 6.8351

>NTDB_id=454835 HUK68_RS01320 WP_175502569.1 273187..274230(-) (pilT) [Comamonas antarctica strain 16-35-5]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMIYDIMSDAQRKVYDEFLEIDFSFEIEGLAR
FRVNAFNQYRGAAAVFRTIPSKILTLEQLNAPKIFAELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTHSFAAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSQNVGMQTLDQN
LTDLVRRNLISPAEARAKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=454835 HUK68_RS01320 WP_175502569.1 273187..274230(-) (pilT) [Comamonas antarctica strain 16-35-5]
GTGGACATCACCCAATTGCTGGCGTTCAGCGTCAAGAACAAGGCCTCCGACCTGCACCTGTCGGCCGGGCTGCCACCGAT
GATCCGCGTGCATGGCGACGTGCGCCGCATCAATGTCGACGCACTCGACCACAAGACCGTCCACGCCATGATCTACGACA
TCATGAGCGACGCCCAGCGCAAGGTCTACGACGAATTCCTCGAAATCGATTTCTCGTTCGAGATCGAAGGCCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAATACCGGGGCGCGGCCGCGGTGTTCCGCACCATTCCCAGCAAGATTCTCACGCTCGA
GCAGCTCAACGCGCCGAAGATCTTCGCCGAACTCGCGCTCAAGCCGCGCGGCCTGGTGCTCGTGACCGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTCGCGGCGATGGTCAACTACCTCAACGAGACCGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACGACTCGAAGAAATGCCTGATCAACCAGCGTGAGGTCGGGCCGATGACGCACTCGTTCGCGGC
CGCGCTGCGCTCGGCGCTGCGCGAGGATCCCGACGCCATCCTGGTCGGCGAAATGCGCGACCTCGAAACCATCCGCCTGG
CCATGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTCGCCGCGCATGAAATCATGCTCGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTCGCGCAGATGTACTCGACCATCCAGACCAGCCAGAACGTGGGCATGCAGACGCTGGACCAGAAT
CTCACCGACCTGGTGCGGCGCAACCTGATCAGTCCCGCCGAAGCCCGCGCCAAGGCCAAGATTCCCGACAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N1WWN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria meningitidis 8013

68.824

97.983

0.674
  pilT Neisseria gonorrhoeae MS11

68.529

97.983

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.742

98.271

0.666
  pilT Vibrio cholerae strain A1552

67.742

98.271

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398